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The efficiency of cronolone sponges in combination with either pregnant mare serum gonadotropin (PMSG) or cloprostenol (PGF2(2α) for inducing and synchronizing the estrous cycle in Turkish Saanen does was investigated during the transition from non-breeding to breeding season. All does (n=80) were treated with 20 mg cronolone sponges for 11 days and divided into 4 equal groups. In addition, each doe received an intramuscular injection of either 1.5 ml sterile saline solution, 0.075 mg PGF2α 500 IU PMSG or 500 IU PMSG and 0.075 mg PGF2α, 24 h before the sponge removal. Cervical artificial insemination (AI) with frozen-thawed semen was performed once 16 h after the detection of the first accepted mount. The total estrous response for the first 24 ± 4 h, total estrous response within 96 h, time to onset of the induced estrus, duration of the induced estrus and pregnancy rate was found to be 75.0%, 97.5%, 31.4 ± 1.2 h, 29.3 ± 1.2 h, and 33.3%, respectively. There were significant differences between the first two groups and the last two groups in terms of the onset of induced estrus and estrous response at the first 24 ± 4 h (P < 0.05). These results indicate that the use of cronolone/PMSG was more effective than cronolone/PGF2α in the attainment of early and compact induction of estrus in Turkish Saanen does.
The aim of this study was to determine the short-, mid- and long-term effects of cloprostenol (a synthetic analogue of prostoglandin F2α, PG) and equine chorionic gonadotropin (E) administration on reproductive organs (uterine tissue and ovaries) in female rats. Three different groups, PG, E, and control (C), were created, as well as six subgroups of the PG and E groups. After the treatment procedure, reproductive organs were removed surgically 7, 14, and 21 days after the last injection. Morphometric and histopathological changes in tissues were evaluated. It was shown that PG and E had a moderate proliferative effect on epithelial cells and endometrial glands, especially in the mid-term. It was also observed that, regardless of the time of application, some pathological changes can result from hormone administration.
In this study the dynamics of follicular growth during estrous cycle after cloprostenol, FGA and PMSG stimulation in goats was compared. On the day 11 of the estrous cycle Group I of the goats (n = 4) was injected with 0.25 mg of cloprostenol for luteolysis stimulation. The follicles which started to grow after treatment formed the ovulatory wave. Group II of the goats (n = 4) was stimulated for 17 days by intravaginal sponges with FGA. After withdrawal of the sponges goats were injected with a single dose of 500 I.U. of PMSG (day 17). Observation of growing follicles on the ovaries were carried out every day through the two consecutive estrous cycles, and was finished on the day of ovulation at the end of second estrous cycle. No differences were observed in total number (4.25 ± 0.5) and the number of the large follicles ≥ 5 mm (2.75 ± 0.5) of the ovulatory wave after cloprostenol injection in comparison to waves of follicles growing during the luteal phase. In Group II of the goats stimulated with sponges impregnated with FGA the number of growing follicles did not differ from the number of follicles in Group I of goats and goats with a natural estrous cycle. After PMSG injection the number of follicles growing in the ovulatory wave increased rapidly. After synchronised ovulation in Group II of the goats follicular cysts were observed and disturbed folliculogenesis of the subsequent estrous cycle. No second wave of follicles was observed and the number of follicles growing was significantly lower (p ≤ 0.05), even in the ovulatory wave, but the ovulation rate did not change in comparison to the natural estrous cycle.
Monoaminooksydaza jest enzymem odpowiedzialnym za deaminację amin katecholowych. Stwierdzono, że jej aktywność w surowicy krwi ssaków ulega znacznym zmianom w przebiegu cyklu rujowego. Celem pracy było określenie wpływu syntetycznego analogu PGF2α na aktyw­ność МАО i jej izoenzymów w surowicy krwi bydła. Badania przeprowadzono na 5 zdrowych klinicznie krowach rasy ncb, którym w 10.-11. dniu cyklu jajnikowego podawano 0.5 mg kloprostenolu. Aktywność całkowitą МАО (MAO-T) oraz aktywność izoenzymów MAO-A i МАО-B w surowicy oznaczano metodą spektrofluorymetryczną wg Matsumoto i in. W pobra­nych co 8 godz. od podania kloprostenolu próbach krwi stwierdzono, że aktywność МАО-B jest ponad dwukrotnie wyższa niż aktywność MAO-A. Najsilniejsze hamowanie aktywności MAO-T oraz MAO-A i МАО-B stwierdzono w okresie okołorujowym, po czym następował powolny wzrost ich aktywności.
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