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Clonal multiplication of Lithospermum canescens [Michx.] Lehm. and Onosma paniculatum [Bur. and Franch]

100%

Syklowska-Baranek K., Pietrosiuk A., Dluska H., Furmanowa M.

Herba Polonica

2004

tom 50

nr 2

An efficient plant micropropagation system was established using shoot tips and stem segments of Lithospermum canescens and Onosma paniculatum (Boraginaceae). A high frequency of axillary shoot formation was achieved on LS [15] medium with 0.5 mg/1 kinetin and 2.0 mg/1 BAP (average shoot formation per explant was 6.18 ± 1.89 for L. canescens and 3.01 ± 0.82 for 0. paniculatum). The best results were obtained with 1x8 clone of L canescens. Axillary branching and root formation were the highest (8.22 shoot formation, 46.7% of rooting). Contamination of the explants with Rhodococcus fascians D188 increased the number of newly formed shoots of 0. paniculatum to 4.6 ± 0.72, while the propagation process of L. canescens was not affected. The highest percentage of rooted shoots of L canescens was observed on V, B5 medium [14] with 0.2 mg/1 1BA (from 27% to 46.7%, depending on plant line), while in the case of regenerated shoots of 0. paniculatum hormone-free LS medium induced roots more effectively (36.7%) than V2 B5 medium with 0.2 mg/1 1BA (25%).

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In vitro propagation of Althaea officinalis: the role of plant growth regulators in morphogenesis

84%

Mujib A., Pipal T., Ali M., Tonk D., Zafar N., Gulzar B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2017

tom 98

nr 3

Ograniczanie wyników

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Herba Polonica

1 Ali M.

1 Dluska H.

1 Furmanowa M.

1 Gulzar B.

1 Mujib A.

1 Pietrosiuk A.

1 Pipal T.

1 Syklowska-Baranek K.

1 Tonk D.

1 Zafar N.

1 2017

1 2004

Wyniki wyszukiwania

Clonal multiplication of Lithospermum canescens [Michx.] Lehm. and Onosma paniculatum [Bur. and Franch]

In vitro propagation of Althaea officinalis: the role of plant growth regulators in morphogenesis