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Endotoxin tolerance in horses - clinical observations

100%

Krumrych W., Dabrowska J., Danek J., Soszynski D.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 2

The studies aimed to induce endotoxin tolerance in horses and to evaluate this state with regard to clinical observations. Investigations involved 8 clinically healthy mares subjected to 4 iv. injections with 0.1 μg/kg b.w. doses of LPS from E. coli each; the three fast doses (LPS-1, LPS-2, and LPS-3) were given daily whereas the fourth injection (LPS-4) was performed one week after LPS-3. Rectal temperature, heart and respiratory rates were measured before LPS administration and 1 h, 2 h, 3 h, 5 h, and 7 h thereafter. The first dose of endotoxin produced a rise in body temperature by 0.8°C after 2 h. However, the consecutive injections weakened temperature response which indicated stimulation of an early-type pyrogenic tolerance. Statistically significant differences between the LPS-1 and LPS-2 responses were found 1 h and 2 h after injection. The administration of LPS-4 produced less visible body temperature alterations suggesting tolerance attenuation after 7 days. Mean respiratory and heart rates were the highest after LPS-1 but no significant differences after subsequent injections were found.

2

Toxocariasis morbidity in Lithuania [1992-1997]

88%

Virbaliene R., Ziliukiene J.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

3

Congenital urachal diverticulum in dogs: a case report

75%

Lojszczyk-Szczepaniak A., Smiech A., Wojnowski T.

Medycyna Weterynaryjna

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2010

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tom 66

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nr 06

421-424

This study reports the case of a 4-year-old female Mastiff dog in which a congenital urachal diverticulum was diagnosed. The disorder was related with atypical clinical manifestations. The animal was referred for a radiological evaluation with the clinical signs of ataxia. The owner stated that the symptoms improved after each spontaneous micturition of a dog. The radiological study revealed the presence of degenerative changes in the lumbosacral spine. Moreover, an abnormal shape of the urinary bladder in the abdominal cavity was observed. The ultrasound imaging showed a large diverticulum in the cranioventral part of bladder. The operative procedure and histopathological analyses have confirmed the presence of a urachal diverticulum. The clinical symptoms completely abated after the surgery.

4

Multiple myeloma - interdiscipline diagnostic problem

75%

Gorska M., Jawniak D., Hus I., Horobowska J.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr Suppl.2, Part 1

Multiple myeloma (MM) is an uncurable plasma cell malignancy, with incidence approximately 4 per 100 000 population and rich symptomatology causing often significant diagnostic problems. In 1992-2004 in Haematology Department of Medical University in Lublin MM was diagnosed in 197 patients (110 woman and 87 men, mean age 61± 11.2 years). The most common monoclonal protein type of was IgG, detected in 133 patients. At the time of initial diagnosis in 138 patients bone pain was a main complain, in 10,2% of these patients it was caused by pathological fractures. In 20 patients (10.2%) clinical course was indolent, and disease was diagnosed accidentally. In the other patients first clinical manifestation were unspecific symptoms, such as: general weeknes (15/197 patients), paraparesis (9/197), acute renal failure (8/197), prolonged infections (8/197) bone of soft tissue tumours (7/197). Taking into account unspecific clinical picture preliminary diagnostics should focus on characteristic for MM laboratory features, i.e.: highly accelerated erythrocyte sedimentation reaction resulted from hyper- and para-proteinaemia; proteinuria; osteolytic lesions in bone radiograms. Lack of appropriate diagnostic strategy prolongs time to therapy commencement to 5.3±4.7 months, in some cases even to 24 months. On the level of basic health care total protein serum level and bone radiograms are adequate diagnostic investigation to establish preliminary diagnosis of MM. In our study increase of total protein serum level and characteristic changes in bone radiograms were found in 96% of patients.

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Length of the styloid process and anatomical implications for Eagle's syndrome

75%

Balcioglu H. A., Kilic C., Akyol M., Ozan H., Kokten G.

Folia Morphologica

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2009

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tom 68

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nr 4

265-270

The styloid process is a bony projection, located just anterior to the stylomastoid foramen, the normal length of which is approximately 20–25 mm. Elongation of the process may cause various clinical symptoms such as neck and cervicofacial pain, described as Eagle’s syndrome. The present study aimed to determine the mean length of the styloid process on cadavers, panoramic radiographs, and dry skulls, and to investigate the incidence of the elongated styloid process, while assessing the elongation in relation to Eagle’s syndrome. When the measurements from the panoramic radiographs were assessed, the mean length of the styloid processes in males and females on the right and left sides were found to be the following: 25.78 ± 5.68 mm; 22.69 ± 3.68 mm, 25.80 ± 5.75 mm; and 22.75 ± 3.65 mm, respectively. The males had greater styloid process lengths than the females, and the differences in length on both the right and left sides were statistically significant. Descriptive statistics and comparison results according to age groups were determined. There was no statistically significant difference between right or left styloid process lengths according to age groups. The mean length of the styloid process of the cadavers and dry bones was 22.54 ± 4.24, and there was no significant difference between the right and left sides of the cadavers and dry bones. The incidence of the elongated styloid process was determined as 3.3%, and the elongations revealed a female dominance. The average length of the elongated styloid process was 36.06 ± 6.12 mm, while the mean length of the styloid processes of the subjects reporting Eagle’s syndrome was 40 ± 4.72 mm. The results of this morphological study will assist clinicians in the diagnosis of Eagle’s syndrome. (Folia Morphol 2009; 68, 4: 265–270)

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Detection of specific lytic and latent transcripts can help to predict the status of Epstein-Barr virus infection in transplant recipients with high virus load

75%

Zawilinska B., Kosinska A., Lenart M., Kopec J., Piatkowska-Jakubas B., Skotnicki A., Kosz-Vnenchak M.

Acta Biochimica Polonica

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2008

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tom 55

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nr 4

693-699

Epstein–Barr virus (EBV), a member of the family Herpesviridae, is widely spread in the human population and has the ability to establish lifelong latent infection. In immunocompetent individuals the virus reactivation is usually harmless and unnoticeable. In immunocompromised patients productive infection or type III latency may lead to EBV-associated post-transplant lymphoproliferative disorder (PTLD). The aim of our research was to investigate the utility of PCR-based methods in the diagnosis and monitoring of EBV infections in bone marrow transplant recipients. Thirty-eight peripheral blood leukocyte samples obtained from 16 patients were analysed, in which EBV DNA was confirmed by PCR. We used semi-quantitative PCR to estimate the viral load and reverse-transcription PCR (RT-PCR) to differentiate between latent and productive EBV infection. In 14 patients we confirmed productive viral infection. We observed a correlation between higher number of EBV genome copies and the presence of transcripts specific for type III latency as well as clinical symptoms.

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A novel mutation A1270G of the EDA1 gene causing Tyr343Cys substitution in ectodysplasin-A in a family with anhidrotic ectodermal dysplasia

75%

Kobielak A., Kobielak K., Biedziak B., Trzeciak W. H.

Acta Biochimica Polonica

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2003

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tom 50

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nr 1

The structure of the EDA1 gene was investigated in a patient with anhidrotic ectodermal dysplasia. Sequence analysis revealed a novel A1270G transition in exon 9 of the EDA1 gene in the patient and his uncle, whereas the patient's mother and grandmother were heterozygotes. This mutation resulted in Tyr343Cys substitution in the extracellular domain of the EDA1 gene product — ectodysplasin-A. The additional Cys343 was located between Cys332 and Cys346 and formed with Cys352 a cluster of four closely situated residues that could potentially form disulfide bonds. This mutation might affect the tertiary structure of the receptor-binding domain of ectodysplasin-A and precipitate the clinical symptoms of anhidrotic ectodermal dysplasia.

8

Infectious encephalitis in Polar foxes

75%

Kopczewski A., Roszkowski J., Zdunkiewicz T., Strzalkowski L., Ulewicz W.

Zeszyty Naukowe. Przegląd Hodowlany

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1996

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tom 28

9

Acute toxicity of sodium salinomycin in Synvertas plv. ad us.vet. preparation in chickens

75%

Neuschl J., Saly J., Simko S., Magic D., Sevcikova Z., Kremen J., Nagy J., Korim P.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 2

The work presents an initial information about degree of toxicological risk for chickens treated with sodium salinomycin in the Slovak anticoccidial (Synvertas plv. ad us. vet., Biotika, Slovenská L’upča, Slovak Republic), under conditions of acute intoxication. Median lethal dose (LD₅₀) of sodium salinomycin in Synvertas plv. ad us. vet. preparation (Biotika) has been determined in 2 and 4-week-old chickens, both sexes, meat type Ross breed. Clinical symptoms of poisoning and patho-morphological changes in dead chickens have been observed as well. Development of clinical symptomatology of poisoning and decrease in intoxication symptoms in 4-weeks-old chickens has been observed even after a single administration of sodium salinomycin in sublethal doses : 20, 30, 40, 50, and 70 mg.kg⁻¹ of body weight (b. w.). LD₅₀ of sodium salinomycin in the objective preparation was calculated on double dose by the interpolation method according to Roth et al. (21) on mortality within 24 h. LD₅₀ value of sodium salinomycin in Synvertas preparation was determined at 102 mg.kg⁻¹ of b. w. (1020 mg of preparation) in 2-week-old chickens and at 106 mg.kg⁻¹ of b. w. (1060 mg of preparation) in 4-week-old ones. From clinical signs of intoxication after lethal doses of 100 and 120 mg of sodium salinomycin per kg⁻¹ of b. w. (1000 and 1200 mg of preparation), the following signs were in the foreground: ataxia, lying on the side or in sternal position with forward stretched neck and legs stretched backward, dyspnoea, cyanosis, sharp CNS inhibition, loss of acoustic and touch reactions. Death occured within 12 h and was accompanied by mild myoclonus symptoms (3-4) with backward stretched limbs. Hyperaemia of the liver, pancreas, spleen and kidneys, anaemia of GIT mucosa, diffuse acute bronchopneumonia, in one chicken lung oedema, slight anaemia of thoracic musculature, slight banding of thigh and thoracic musculature were recorded in most dead chickens. The toxic effect of sodium salinomycin started to manifest itself in 4-week-old chickens after sublethal dose of 20 mg.kg⁻¹ of b. w. (200 mg of preparation). Poisoning symptoms were very weak and disappeared after 15 h.

10

Various types of hypophosphataemia in dairy cows and the clinical implications depending on the intensity of the deficiency

63%

Kurek L., Lutnicki K., Banach A.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 1

35-41

The paper aimed at defining the specific biochemical parameters, as well as the use of treatment methods, according to types and intensity of the clinical signs of non-complicated hypophosphataemia in cows. The investigations were carried out on 300 HF cows in 15 herds in the Lublin Region. The mean milk yield in the herds was 36.8 ±2.8 dl. The cows were 3-9 years old and had a similar diet ratio. The animals with hypophosphataemia symptoms in the early stadium (2-7 d) were divided into three groups according to the intensity of clinical signs whereas the control group consisted of cows with no clinical signs of the illness. The inorganic phosphorus (Pi), total Ca, total Mg, K, Fe, total protein, Cu, bilirubin, FFA, and creatinine concentrations, as well as AST and AP activity, were determined in serum and plasma. The animals with markedly exhibited signs (recumbency and tremor) showed significantly decreased Pi, Mg, and FFA contents and simultaneously increased activity of AST and AP. In the group exhibiting minimal signs (group I), there were no significant disturbances in homeostasis except the low Pi concentration, so it was possible to use the specialised phosphorus preparations in the treatment. In the other groups (II, III), which exhibited more marked signs of the illness, it was necessary to improve the parenchymal organs functions in addition to phosphorus level normalisation.

11

Interval hypoxic training in complex treatment of Helicobacter pylori-associated peptic ulcer disease

63%

Semen K. O., Yelisyeyeva O. P., Kaminskyy D. V., Cherkas A. P., Zarkovic K., Lutsyk O., Cipak A., Jaganjac M., Zarkovic N.

Acta Biochimica Polonica

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2010

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tom 57

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nr 2

This study was aimed to demonstrate the efficacy of interval hypoxic training (IHT) in complex treatment of Helicobacter pylori-associated duodenal peptic ulcer disease (DPUD) by parameters of aerobic metabolism and indexes of heart rate variability (HRV). Eighty patients with H. pylori-associated DPUD were included into the study, mean age 32±1.8 yrs, duration of the disease up to 10 years (66.3 %). IHT was modulated using Frolov's hypoxicator (TDI-01) for 30 days after standard eradication therapy. Daily hypoxic sessions consisted of three one-minute sessions, one two-minute, and one three-minute sessions separated by one-minute intervals of room-air breathing. Use of IHT resulted in more efficient elimination of clinical symptoms, histological hallmarks of inflammation and signs of oxidative stress in glandulocytes of the gastric mucosa as determined by 4-hydroxynonenal accumulation. Moderate prooxidant activity of IHT was demonstrated by the increased level of TBARS and oxidatively modified products, normalization of hydroperoxides, middle mass molecules and atherogenic beta-lipoproteins with simultaneous increase in catalase activity and mild decline of SOD activity. Therefore, IHT appeared to be accompanied by higher intensity of redox reactions and enhanced regeneratory processes in cells and tissues. Significant increase in HRV was also noted. Such changes were associated with reduction of inflammation signs and modulation of the autonomic homeostasis in DPUD patients. In general, use of IHT in complex treatment of H. pylori in DPUD patients can be recommended to increase resistance to oxidative stress and to modulate autonomic balance and oxidative homeostasis

12

Evaluation of the frequency of Pneumocystis jirovecii occurrence in a group of children hospitalized for acute respiratory infections

63%

Gołab E., Rozej-Bielicka W., Pancer K.

Wiadomości Parazytologiczne

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2011

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tom 57

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nr 2

Primary infection with Pneumocystis jirovecii in small children may cause inflammation of the respiratory tract which requires hospitalization. Lack of characteristic clinical symptoms makes it impossible to recognize P. jirovecii infections without performing laboratory analyses. Nasopharyngeal swabs from 70 children with respiratory tract infections were screened for fragments of the P. jirovecii genome. Pneumocystis DNA was found in swabs taken from two (2.9%) of the tested children: a newborn who was infected in the hospital and a six month old baby admitted to hospital two days after pneumonia was diagnosed. The obtained results confirm that primary P. jirovecii infections may occur in the form of acute respiratory tract inflammations suggesting a viral infection. In differential diagnosis of Pneumocystis infections in children molecular methods are useful as their high sensitivity makes it possible to analyze samples obtained in a non-invasive way.

13

Pictorial review of COVID-19 in the Lublin Region – Imaging disease progression with CXR and CT

63%

Kurys-Denis E., Podgorska K., Prystupa A.

Journal of Pre-Clinical and Clinical Research

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2021

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tom 15

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nr 1

14

Subtelomeric rearrangements in Polish subjects with intellectual disability and dysmorphic features

63%

Bogdanowicz J., Pawlowska B., Ilnicka A., Gawlik-Zawislak S., Jozwiak A., Sobiczewska B., Zdzienicka E., Korniszewski L., Zaremba J.

Journal of Applied Genetics

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2010

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tom 51

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nr 2

215-217

Fluorescent in situ hybridization (FISH) was performed in 76 patients referred to our department because of intellectual disability and dysmorphic features that can be related to subtelomeric microaberrations. In all the patients, conventional cytogenetic methods revealed normal karyotype. Four (5.3%) subtelomeric rearrangements were detected by FISH: 2 subtelomeric 1p36 deletions, an unbalanced translocation involving chromosomes 1 and 12 with 1p36 deletion, and a de novo balanced translocation involving chromosomes 19 and 22. Thus, 3 cases of 1p36 subtelomeric deletion were found (3.95%). To confirm subtelomeric rearrangements in 2 patients, comparative genomic hybridization (CGH) was applied. Moreover, 3 cases of polymorphism without phenotypic effects were found: in 2 patients, the polymorphism involved the long arm of chromosome 2 (maternal derivative in both patients), while in the third patient, a polymorphism of the long arm of chromosome 7 was diagnosed. The latter polymorphism was also found in the patient's mother and grandfather.

15

Role of the unfolded protein response in the pathogenesis of Parkinson’s disease

63%

Varma D., Sen D.

Acta Neurobiologiae Experimentalis

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2015

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tom 75

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nr 1

Parkinson's disease is the second most common neurodegenerative disease which affects almost 1% of the population above the age of 60. It is is characterized by loss of dopaminergic neurons in the striatum and substantia nigra, coupled with the formation of intracellular Lewy bodies in degenerating neurons. Recent evidence suggests endoplasmic reticulum stress as a common and prominent occurrence in the progression of Parkinson's disease pathogenesis in the affected human brain. One of the cellular defense mechanism to combat endoplasmic reticulum stress due to excessive protein accumulation is through activation of the unfolded protein response pathway. In this review we focus on the impact and role of this unfolded protein response as a causative factor of Parkinson's disease leading to neurodegeneration.

16

The first clinically manifested case of angiostrongylosis in a dog in Slovakia

63%

Miterpakova M., Hurnikova Z., Zalewski A. P.

Acta Parasitologica

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2014

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tom 59

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nr 4

The first clinical case of canine angiostrongylosis from Slovakia, previously infection-free country, is described. 18-month old male Bernese mountain dog living in south-eastern part of Slovakia showed poor health condition characterized by weight loss, irritating cough, dispnoe, intense salivation, vomiting and bilateral scleral bleeding. Two times even the acute physical collapse occurred. Blood analysis was provided and revealed increase of total protein, eosinophilia, monocytosis, and mild thrombocytopenia. Anaemia characterized by reduced number of erythrocytes and reduced levels of haemoglobin, packed cell volume and iron was also diagnosed. Larvoscopic Baermann technique revealed the presence of Angiostrongylus first stage larvae. Infected dog excreted larvae in high numbers — in 10 g of the faecal material more than 800 larvae were counted. DNA analysis using PCR confirmed the presence of Angiostrongylus vasorum species. The first clinical case of angiostrongylosis has evidenced that the new life-threatening parasitic disease of dogs has spread to the territory of Slovakia. A serious effort is therefore inevitable to increase the professional awareness and knowledge on diagnosis, treatment and prevention.

17

DNA methylation analysis of a de novo balanced X;13 translocation in a girl with abnormal phenotype: evidence for functional duplication of the whole short arm of the X chromosome

63%

Myszka A., Karpinski P., Makowska I., Lassota M., Przelozna B., Slezak R., Sasiadek M. M.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

We report on a 13-month-old girl showing dysmorphic features and a delay in psychomotor development. She was diagnosed with a balanced de novo translocation 46,X,t(X; 13)(p 11,2; p 13) and non-random inactivation of the X chromosome. FISH analysis, employing the X chromosome centromere and XIST-region-specific probes, showed that the XIST locus was not involved in the translocation. Selective inactivation of paternal X, which was involved in translocation, was revealed by the HUMARA assay. The pattern of methylation of 5 genes located within Xp, which are normally silenced on an inactive X chromosome, corresponded to an active (unmethylated) X chromosome. These results revealed that in our proband the X chromosome involved in translocation (Xt) was preferentially inactivated. However, genes located on the translocated Xp did not include XIST. This resulted in functional Xp disomy, which most probably accounts for the abnormal phenotype in our patient.

18

Zapalenie zatok okołoodbytowych

63%

Dobrzynski A.

Weterynaria w Praktyce

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2020

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tom 17

|

nr 06

19

The influence of experimental Yersinia enterocolitica infection on the pregnancy course in sows - preliminary studies. I. Bacteriological examination

63%

Platt-Samoraj A., Szweda W., Procajlo Z.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 3

The aim of the study was to determine the influence of Yersinia enterocolitica (Y. enterocolitica) experimental infection on the carrying and shedding states of the microorganism by pregnant sows and on the bacteria occurrence in tissues of the infected animals and aborted or stillborn piglets. Twelve pregnant sows were divided into 4 groups and infected per os on 33 – group I (n=3), 54 – group II (n=3) and 89 – group III (n=3) day of pregnancy with the Y. enterocolitica strain isolated from the palatine tonsil of aborted swine fetus. The control group (n=3) remained uninfected. Rectal, oral and vaginal swabs from sows, placentas and the specimens of tissues from stillborn piglets were collected for bacteriological examination. Eight weeks after delivery, the sows were slaughtered and samples of internal organs were subjected to bacteriological examination. Pregnancy in all groups of sows took a normal course, and no cases of abortion were observed. Y. enterocolitica was isolated from oral, rectal and vaginal swabs of all infected sows. The number of stillborn piglets in the litters was highest in group III, where two macerated fetuses with putrefactive lesions were found. The bacteria were isolated from tissues of stillborn piglets in groups I and III, and only from placenta in group II. In rectal swabs of piglets in all groups, Y. enterocolitica was not isolated. The results of experimental infection of pregnant sows with Y. enterocolitica revealed that in animals infected in the last part of pregnancy, the microorganisms were isolated most frequently from vaginal, rectal and oral swabs of sows as well as from internal organ tissues of stillborn piglets.

20

Malaria - the formidable enemy of humanity

63%

Pawar A., Pal A.

International Letters of Natural Sciences

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2015

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tom 43

Malaria, a disease caused by protozoan parasite of the genus Plasmodium, is an ancient enemy of the human race. Despite enormous efforts to control, it is still a main cause of morbidity and mortality in many endemic countries of the world, particularly Africa, South of Sahara. Malaria seems to be unstoppable due to lack of an effective vaccine, acquisition of drug-resistance by malaria parasite, and insecticide resistance by Anopheles mosquitoes. In this scenario, along with search for novel preventive approaches, adequate health facilities, health literacy and awareness among people are required to control and eliminate this formidable disease from society.

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