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Autolysis of Listeria monocytogenes strains isolated from clinical and food specimens were tested under starvation conditions. Late exponential phase harvested bacterial cells were transferred in potassium phosphate buffer (100 mM, pH 7) and incubated at 37°C to induce lysis. At the end of the 48 h incubation period a heterogeneous autolysis profile was observed among the tested strains. All the clinical strains exhibited high autolysis i.e. above 75%. Meanwhile, the extent of food isolates was not as high as the clinical ones but demonstrated a great variability - ranging between 22 to 88%. An increase in the amount of intracellular markers upon lysis was also measured in the higher autolytic clinical strain - UVF172, compared to low autolytic food strain - UVF114. The results obtained from the study provided evidence that autolysis in L. monocytogenes is strain dependent and revealed that tested clinical isolates had a higher level of autolysis than food isolates.
Enterococcal infection has become a major clinical problem and E. faecalis and E. faecium are the most frequently isolated species. However, the isolation of other species (E. casseliflavus, E. gallinarwn, E. durans) from clinical materials was reported recently. The aim of this study was to evaluate drug resistance of 97 species of enterococci isolated from clinical specimens of Upper Silesian Health Center of Child and Mother in Katowice. Each strain was tested for susceptibility to vancomycin, teicoplanin, aminoglycosides (gentamycin and streptomycin) and synercid (quinupristine/ dalfopristine) by the E-test method. Fifty three percent of studied enterococci demonstrated high level aminoglycoside resistance (HLAR) (MIC > 1024 μg/ml). Sixty three strains of E. faecalis were sensitive to vancomycin (MIC 1-4 μg/ml), but 5 strains demonstrated low sensitivity (3 strains with MIC = 6 μg/ml and 2 strains with MIC = 24 μg/ml). All studied enterococci were sensitive to teicoplanin (MIC < 2 μg/ml). A high percentage of E. faecium (70%) resistant to synercid was demonstrated (MIC = 2-24 μg/ml). Infection control and monitoring of antibiotic sensitivity among isolated hospital strains may prevent the transmission of resistant strains in a pediatric hospital.
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