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1

The utility of Caco-2 cells in isolation of enteroviruses from environmental and clinical material

100%
Wieczorek M., Ciacka A., Witek A., Litwinska B.
Polish Journal of Microbiology
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2014
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tom 63
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nr 1
The work presented here demonstrates the utility of Caco-2 cells in the isolation of enteroviruses (EVs) from environmental and clinical materials. Thirty-two samples of cerebrospinal fluid positive in Pan-entero RT-PCR were taken for EV strain isolation in cell culture. Out of the 32 samples analysed, 22 (68.75%) were positive for enteroviruses by isolation in Caco-2 cells, and 10 (31.25%) were positive by isolation in RD cells. High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells. We proved that Caco-2 cells were more effective than RD cells in enterovirus isolation, irrespective of the material used in the inoculation process.
2
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Genetic diversity within Echinococcus multilocularis isolated from clinical and environmental material in Poland - preliminary investigations

100%
Szostakowska B., Rebala K., Sulima M., Lass A., Myjak P.
Annals of Parasitology
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2016
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tom 62
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nr Suppl.
3

Diagnosing and management of iatrogenic moderate and severe ovarian hyperstymulation syndrome [OHSS] in clinical material

80%
Jakimiuk A. J., Fritz A., Grzybowski W., Walecka I., Lewandowski P.
Folia Histochemica et Cytobiologica. Supplement
|
2007
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tom 45
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nr 1
105-108
4

Study on properties of enterohaemorrhagic Escherichia coli 0157 isolates and their survival under different conditions

80%
Kovalikova M., Pipova M., Kantikova M., Kozarova I., Janosova J., Martonova M., Kovalik M.
Medycyna Weterynaryjna
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2008
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tom 64
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nr 05
During the period of 2004-2006, 955 samples of food and clinical material were collected from the Slovak Republic and Belgium. Of the total number of 216 food samples originating from the Slovakia territory, the authors obtained 5 isolates (2.31%) of Escherichia coli O157. Three E. coli O157 (2.30%) isolates were obtained during the examination of 130 samples of slaughter animals from the Belgium territory. In Slovakia no occurrence of E. coli O157 was proved in any sample of clinical material or environment (altogether 36 samples). On the contrary, of 573 clinical and environmental samples from Belgium the presence of the respective pathogen was proved in 50 cases (8.73%). The authors studied some attributes of the recovered E. coli O157, confirmed by immunomagnetic separation (IMS) and polymerase chain reaction (PCR). The results indicate that the isolates are capable of surviving for 15 min at 70°C. The acidic environment, which is characteristic for fermentation of cheese (pH 4-4.5), had no devitalisation effect. The E. coli food isolates survived in a wide range of pH (2-11) while with clinical isolates the pH range of survival was from 2.5 to 10.5. The addition of NaCl in concentrations ordinarily used in food industry (3-6%) failed to inhibit the growth of pathogens.
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