The aim of the study was to characterise the problem of road accidents involving selected species of wild-living animals present in the area of Lublin. Another aim of the investigations was to determine the relationship between selected behavioural elements of the annual life cycle of animals and the incidence of wildlife-vehicle collisions. The information about wildlife-vehicle collisions was obtained from the documentation held by the shelter for homeless animals in Lublin and veterinary services. The data are presented in a spreadsheet and analysis was performed in the GIS (ArcGIS 10.1) environment. Based on the ESRI Base Map-BING MAP HYBRYD, a vector database of streets where the incidents had taken place was compiled. Each street was assigned the number of incidents recorded in the period of 2009–2012. Animals were divided into three groups: large mammals, small mammals and bats. The compiled data indicate the highest road mortality among roe deer (132), foxes (63), and martens (33). The reported results show the greatest number of wildlife-vehicle collisions on exit roads leading from the city centre. Presumably, this may be associated with the lack of speed limits and possible faults in the infrastructure arising already at the design stage.