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Phenolic acids from herb of Linaria vulgaris (L.) Mill, were analysed by means of two di­mensional thin layer chromatography and RP-HPLC method. Ten acids were identified: protocatechuic, gallic, caffeic, p-hydroxybenzoic, p-coumaric, vanillic, ferulic, homoprotocatechuic, salicylic and o-hydroxyphenylacetic. By the RP-HPLC method the content of five major compounds in fraction of free phenolic acids was determined.
Scutellaria barbata D.Don is an important medicinal plant used in traditional Chinese phytotherapy. It has been recently acclimated with success to Central European climatic conditions. Here, we present the results of chromatographic studies of herb collected from plants cultivated in Poland with respect to polyphenols compounds. By means of TLC and HPLC analyses we were able to detect several flavonoids: baicalein, luteolin, wogonin, apigenin, quercetin and kaempferol as well as ellagic and p-coumaric acids. From this study we can conclude a satisfactory quality of the crude drug from acclimated plants.
Phenolic acids from herbs of two genuses Carex were analysed by Thin-Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Ten following phenolic acids were identified: caffeic, protocatechuic, gentisic, p-coumaric, p-hydroxybenzoic, p-hydroxyphenylacetic, synapic, ferulic, syringic, and vanilic. These compounds were identified by comparison with authentic standards. By the RP-HPLC method the content of seven phenolic acids (caffeic, protocatechuic, p-coumaric, p-hydroxybenzoic, ferulic, syringic, and vanilic) was determined.
Field bindweed (Convolvulus arvensis L.) has an impressive array of survival techniques, due to which it has become one of the worst weeds. This persistent field darnel is native to Eurasia but has spread to many parts of the world. TLC and HPLC were applied to carry out qualitative and quantitative determination of polyphenols compounds in bindweed, such as coumarins and phenolic acids: free and liberated by acid and alkaline hydrolysis. We identified and confirmed umbelliferone and scopoletin in the coumaries fraction. In the fraction of phenolic acids we noticed the occurrence of protocatechuic, caffeic, chlorogenic, gentisic, p-coumaric, p-hydroxybenzoic, p-hydroxyphenylo- acetic, ferulic, vanillic and salicylic. The caffeic (31.24 mg%), vanillic (5.8 mg%) and protocatechuic (4.62 mg%) acids were found to be the dominant compounds in the analysed plant.
Celem naszej pracy było określenie zawartości procentowej i składu chemicznego olejków eterycznych występujących w kwiatostanach, liściach i kłączach Silphium perfoliatum L. Zawartość procentową olejku oznaczano metodami destylacji z parą wodną w aparacie Derynga wg FP IV i FP V. Skład olejków eterycznych otrzymanych z kwiatostanów i liści jest podobny z tym, że w przypadku kwiatostanów dominującym związkiem w olejku jest a-pinen (20,9%), zaś w przypadku liści głównym składnikiem jest tlenek kariofilenu (34,7%). Natomiast skład olejków eterycznych otrzymanych z kłączy różni się istotnie od składu olejków otrzymanych z liści i kwiatostanów.
In this study the results of chromatographic analysis of phenolic acids isolated from vegetative and generative parts of X. strumarium L. are presented. The plants were collected in Pharmacognostic Garden, Lublin, Poland. Free phenolic acids and those released after acidic and basic hydrolysis were subjected to TLC and HPLC analyses. The following seven phenolics were identified: caffeic, gallic, protocatechuic, chlorogenic, vanillic, syringic and ferulic acids. The percentage content of phenolics is about 0.2393-0.2815% for herb, 0.2395-0.26627% for fruit, and 0.09138-0.09393% for root.
The host-plants of bird cherry-oat aphid were compared as to the content and composition of phenolic compounds. The gallic, chlorogenic, caffeic, siringic, o- and p‑coumaric, tannic, ferulic, sinapinic, salicylic, p‑hydroxybenzoic acids and /+/‑catechin were detected in the all studied host-plants of the bird cherry-oat aphid. Coumarin and vanilic acid only occurred within orchard grass and winter triticale tissues. Predominant compounds within P. padus leaves were /+/‑catechin, chlorogenic acid, p-hydroxybenzoic acid and tannic acid. Vanilic and salicilic acids were the major compounds of the orchard grass, whereas leaves of winter triticale contained mostly salicilic, chlorogenic and vanilic acids as well as coumarin.
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