Wyniki wyszukiwania

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The nuclear protein p30 specifically interacts with a nuclear matrix attachment region from the rat genome

100%

Fedorov A., Lukyanov D., Rogolinski J., Widlak P., Podgornaya O., Rzeszowska-Wolny J.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 1

In our previous study, a 454 bp DNA fragment was isolated from rat genomic DNA as an element which interacts with nuclear matrix proteins, i.e. a Matrix Associated Region (MAR). Computer analyses revealed that the right half of this fragment, named RME (Rat MAR Element), possesses a high matrix association potential and is likely to be responsible for the matrix association of the whole sequence. RME was used as a probe in an electrophoretic mobility shift assay (EMSA), and with the use of Southwestern blotting, a rat liver nuclear protein which binds specifically to it was identified. Its molecular mass was estimated by SDS-PAGE as 30 kDa (p30). Polyclonal antibodies raised against protein-RME complexes caused a super-shift of specific complexes in EMSA, and bound to p30 in nuclear extracts of rat liver in Western blotting. The immunofluorescence labelling of a rat embryonic fibroblast cell monolayer with anti-p30 antibody revealed a mainly intranuclear pattern of staining.

2

Mammalian DNA methyltransferases

100%

Siedlecki P., Zielenkiewicz P.

Acta Biochimica Polonica

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2006

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tom 53

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nr 2

DNA methylation is an epigenetic process affecting gene expression and chromatin organization. It can heritably silence or activate transcription of genes without any change in their nucleotide sequences, and for a long time was not recognized as an important regulatory mechanism. However, during the recent years it has been shown that improper methylation, especially hypermethylation of promoter regions, is observed in nearly all steps of tumorigenesis. Aberrant methylation is also the cause of several major pathologies including developmental disorders involving chromosome instabilities and mental retardation. A great progress has been made in our understanding of the enzymatic machinery involved in establishing and maintaining methylation patterns. This allowed for the development of new diagnostic tools and epigenetic treatment therapies. The new approaches hold a great potential; several inhibitors of DNA methyltransferases have already shown very promising therapeutic effects.

3

The chicken alpha- and beta-globin gene domains and their chromatin organization

84%

Recillas-Targa F.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 4

451-467

The eukaryotic genome is organized into discrete chromatin domains. The globin groups of genes have been two of the classical biological systems to study the relationship between gene regulation and chromatin structure during development. The individual promoters, enhancers and silencers of the globin genes are stage- and tissue-specific regulatory elements that are controlled by the interaction of ubiquitous and erythroid nuclear factors. Such regulated activation requires an optimal chromatin organization. Erythroid and constitutive DNase I hypersensitive sites (DHs) contribute to chromatin domain remodeling mediated by locus control region (LCR) activity and defined by domain boundaries. A comparative analysis of the chicken α-and β-globin domains will outline the relevance and effect of chromatin structure on gene regulation.

4

Heterochromatin organization in metaphase chromosomes and interphase nuclei of Dasypyrum breviaristatum [Lindb f] Frederiksen

84%

Pignone D., Galasso I., Blanco A., Cremonini R.

Acta Societatis Botanicorum Poloniae

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2000

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tom 69

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nr 1

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

1 Acta Biochimica Polonica

1 Acta Societatis Botanicorum Poloniae

1 Blanco A.

1 Cremonini R.

1 Fedorov A.

1 Galasso I.

1 Lukyanov D.

1 Pignone D.

1 Podgornaya O.

1 Recillas-Targa F.

1 Rogolinski J.

1 Rzeszowska-Wolny J.

1 Siedlecki P.

1 Widlak P.

1 Zielenkiewicz P.

1 2006

1 2004

2 2000

The nuclear protein p30 specifically interacts with a nuclear matrix attachment region from the rat genome

Mammalian DNA methyltransferases

The chicken alpha- and beta-globin gene domains and their chromatin organization

Heterochromatin organization in metaphase chromosomes and interphase nuclei of Dasypyrum breviaristatum [Lindb f] Frederiksen