Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 58

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  chromatin
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
The treatment of agarose embedded plant nuclei by strong protein dénaturants was demonstrated to result in discrete self-fragmentation of intact nuclear DNA. The set of resultant DNA cleavage products involves two main types of DNA fragments sized about 50-100 kb and 300-500 kb, being of the same type in various eukaryotic representatives. The pattern of ordered DNA fragmentation has been shown to be similar both in intact nuclei and in histone-depleted ones thus suggesting that the observed DNA fragments represent preexisting DNA structural domains, corres­ponding to the higher levels of chromatin folding. The topoisomerase II-speeific poison teniposide (VM-26) has been shown to increase the ordered DNA cleavage while the conditions stimulating the topoisomerase II-mediated reverse reaction lead to the reassociation of the cleaved DNA domains. The data presented suggest that the nuclear DNA structural domains are involved in functioning of the topoisomerase II/DNA complex, the main property of which is its ability to mediate the cleavage/ /reassociation reactions.
The rat hst70 gene is specifically expressed in spermatocytes and spermatids. This tissue-specific expression of the gene is primarily mediated through cis-acting elements located within the 0.4 kb segment upstream of the coding region, including two transcription initiation sites. Here, we study the 5’ and 3’ distal elements flanking the hst70 gene and find that they possess structural motifs characteristic of MAR/SAR elements, and exhibit enhanced affinities for nuclear matrix binding in vitro. Such elements bind efficiently to matrices from either the testis or the liver, i.e. tissues where the gene is either fully active or repressed, although one subfragment in the 5’ region was identified as exhibiting testis-specific interactions. Surprisingly, the activity of the CAT reporter gene was repressed in testis-transient transfection assays when the hst70 promoter sequences were extended into the 5’ MAR/SAR.
Nuclear receptors arc ligand-dependent transcription factors which can also be ac­tivated in the absence of their lipophilic ligands by signaling substances acting on cell membrane receptors. This ligand-independent activation indicates the impor­tance of nuclear receptor phosphorylation for their function. Nuclear receptor- mediated transcription of target genes is further increased by interactions with re­cruited coactivators forming a novel family of nuclear proteins. CBP/p300, a coacti­vator of different classes of transcription factors, including the tumor suppressor protein p53, plays a special role acting as a bridging protein between inducible tran­scription factors and the basal transcription apparatus, and as an integrator of di­verse signaling pathways. Coactivators of nuclear receptors and associated proteins forming a multicomponent complex have an intrinsic histone acetylase activity in contrast to nuclear receptor and heterodimer Mad-Max corepressors, which recruit histone deacetylase. Similarly the Rb protein interacts with histone deacetylase to re­press transcription of cell cycle regulatory genes. Targeted histone acetylation/dca- cetylation results in remodeling of chromatin structure and correlates with activa­tion/repression of transcription. Recent data point to the important role of coactiva­tor proteins associated with inducible transcription factors in transcription regula­tion, and in the integration of multiple signal transduction pathways within the nu­cleus.
Long non-coding RNAs (lncRNAs) are series of transcripts with important biological functions. Various diseases have been associated with aberrant expression of lncRNAs and the related dysregulation of mRNAs. In this review, we highlight the mechanisms of dynamic lncRNA expression. The chromatin state contributes to the low and specific expression of lncRNAs. The transcription of non-coding RNA genes is regulated by many core transcription factors applied to protein-coding genes. However, specific DNA sequences may allow their unsynchronized transcription with their location-associated mRNAs. Additionally, there are multiple mechanisms involved in the post-transcriptional regulation of lncRNAs. Among these, microRNAs might have indispensible regulatory effects on lncRNAs, based on recent discoveries.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.