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Effects of 24-epibrassinolide (EBR) treatments on chlorophyll (Chl) degradation and postharvest quality of two lime (‘Persian lime’ and ‘Tahiti’) cultivars during chilling-induced storage were studied in this work. EBR at 0, 2.5, 5 and 10 µM were applied to fruit by vacuum infiltration at 30.63 kPa for 7 min and afterward stored at 4°C for 60 days. Postharvest EBR application effectively maintained greater fruit firmness and lessened weight loss during cold storage. The results displayed that EBR treatments efficiently delayed the reduction of the Chl a contents and hue values. EBR treatments significantly increased ascorbic acid content, total phenolic content (TPC), antioxidant activity, and titratable acidity (TA). On the other hand, the treatments reduced soluble solids concentration (SSC), pH, and SSC/TA ratios during cold storage. Overall, EBR application by vacuum infiltration could be an effective and simple method for maintaining postharvest quality of limes during cold storage.
Chlorophyll degradation occurring during leaf senescence in under control of plant hormones. Changes in the chlorophyll content and the effects of BA (benzyladenine) and GA₃ (gibberellic acid) on this process were analyzed during senescence of cut leaves of Zantedeschia aethiopica Spr. and Zantedeschia elliottiana Engl., two species grown for the florists’ green. Both growth regulators were applied as 24 h pulse treatment: 0.25 mmol·dm⁻³ and 0.1 mmol·dm⁻³ for GA₃ and BA, respectively. Gibberellic acid was more effective than benzyladenine in delaying senescence of both Zantedeschia species leaves by retarding chlorophyll loss. A standard preservative solution used to prolong longevity of cut flowers (8-HQC /citrate of hydroxyquinoline/ +2% S /sucrose/) radically accelerated chlorophyll loss of Z. aethiopica leaves but had no effect on pigment degradation in leaves of Z. elliottiana. However, in both species the preservative diminished the positive effect of GA₃ treatment on final chlorophyll content.
Colour is traditionally one of the important appearance features of all fruit for consumers in deciding to buy them. Colour is therefore important in the postharvest supply chain. But where does that colour of fruit come from? Clearly the period of growing and the circumstances during growth are important for developing this im­portant feature. During several seasons (2007-2009), the skin colour of individual apples of dif­ferent cultivars ('Braeburn', 'Fuji', 'Gala', 'Golden Delicious') were measured using a Minolta CR-400 chromameter during the last 40-60 days before (commercial) har­vest. By including the biological variation between individual apples in the analyses and applying non linear indexed regression analysis based on process oriented mod­els, explained parts were obtained for the a*-value, all exceeding 90%. The estimated rate constants for the colouration process were remarkably similar for all cultivars (except 'Fuji') and growing conditions. That would indicate that the process of colouration is really reflecting the degradation of chlorophyll and not the production of red or yellow coloured blush (anthocyanins). The expected effect of growing conditions (fertilization and crop level, hail net or not, sunny side or shady side of the tree) did change the mechanism nor the kinetic parameter values but could all be attributed to the minimal obtainable skin colour (asymptotic values of the logis­tic model). This type of information from the production period may constitute an important link to postharvest supply chain management.
The influence of air pollution on epiphytic lichens in Košice city has been studied. We observed differences in number of species as well as lichen abundance at sites close to a steel factory south of the city, the city center, and peripheral parts north of the city. For the city center and sites close to steel factory, lichens more tolerant to pollution from Zone 3-4 were typical. However, on the north periphery of the city (site “Alpinka”) we found even Ramalina fastigiata, a typical member of zone 7, which include lichens very sensitive to air pollution [30]. We demonstrated by chlorophyll analysis of transplanted Hypogymnia physodes, that chlorophyll a degradation (expressed as ratio of OD 435/OD 415) negatively correlates with degree of lichen diversity and abundance at the studied sites. Using EDX-microanalysis we determined amounts of elements in lichen thalli of Lecanora chlarotera, Physcia tenella and bark of the tree Populus tremula (lichen substrate) near U.S. Steel in Košice due to determine the chemical nature of air pollution. Similarly, we analyzed the amount of these pollutants in control lichens Flavoparmelia caperata, Ramalina fastigiata and Physcia aipolia, grown in northern peripheral parts of the city. We demonstrated possibilities to parallel the use of several methodologies in assessment of air pollution by lichens in urban areas with intensive industry.
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