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Plant height and the size of leaf blades are of considerable importance in the cultivation of spice plants in pots, as these parameters are decisive in determining the proper harvest time of such plants. Light is one of the factors affecting to a significant degree the morphogenesis of plants. The goal of this study was to estimate to what extent different light quantities reaching plants can influence their height and leaf area index (LAI) as well as the concentrations of essential oils and chlorophyll in leaves. The experiments were conducted from July to the middle of August 2004 and 2006 in a glasshouse of the Marcelin Experimental Station. The object of the performed investigations included five species of spice plants: dill cv. Ambrozja (Anethum graveolens L.), garden chervil (Anthriscus cerefolium L. Hoffm.), garden rocket (Eruca sativa Lam.) parsley cv. Titan (Petroselinum crispum Mill. subsp. crispum) and salad onion cv. Sprint (Allium cepa L.). The following amounts of light access to plants were assumed: 100% in the treatment without shading and 70 and 60%. After 7, 14, 21 and 28 days from plant emergence, the following measurements were taken: plant height and leaf area, whereas after the last week of vegetation, the content of chlorophyll and essential oils in air-dry leaves was also determined. On the basis of the performed investigations it was found that plants of all the examined species were characterised by the fastest growth and greatest height at 60% access to light. The leaf area index of all experimental plants was also the biggest at the same exposure to light. The highest concentrations of essential oils were determined in the leaves of dill, leaf parsley and chervil when they were cultivated at 60% light access. Differences in the available light quantity failed to affect the relative chlorophyll content in the leaves of parsley and garden rocket. In the case of chervil, leaf chlorophyll was found lower in the treatment with 70% light exposure.
The relationship between the photoelectric reaction of Zea mays L. plants and concentrations of cadmium (10-2 M - 10-6 M) in the external medium was investigated. The experiments were carried out by using the conventional electrophysiological technique. The effect of cadmium ions on the acidification of the external medium by fragments of leaves has also been studied. Our results suggest that the photoelectric reaction in plants treated with cadmium ions was significantly reduced. The effect of cadmium ions on the photoelectric reaction at low concentrations (10-6 M, 10-5 M and 10-4 M) was mainly quantitative. High sublethal concentrations of this metal (10-3 M and 10-2 M) induced strong inhibition on these reactions. Measurements of pH of the incubation medium showed that cadmium-dec - reased light induced acidification by Zea mays L. leave cells and that the H+ electrogenic proton pump probably participated in generation of the photoelectric response in the green plant cells. Moreover, the correlation between the electric potential value and decrease in chlorophyll content in mesophyll cells was studied.
The aim of the present study was to examine the influence of spectral composition of irradiation (white - WL or white supplemented by blue radiation - WBL) and ozone on chlorophyll content and the optical properties of broccoli leaves (Brassica oleracea var. botrytis cymosa). Broccoli leaves were injured by the ozone dose applied (70 ppb, 6 hours daily) mainly under WBL. Leaf lamina damage was much larger in the outer, physiologically older part than in the inner, physiologically younger part. Therefore, further studies were carried out separately on leaf fragments from the outer(OP) and the inner parts (IP). The spectrophotometric analysis did not reveal significant differences in the chlorophyll content between treatments. The variations in reflectance in the visible region (400-700 nm) were generally not significant with respect to tissue age (OP, IP), ozone fumigation and growth conditions (WBL, WL). Only in plants grown under WBL there was an increase in green reflectance in ozone-treated leaves observed, which indicates a decrease in the chlorophyll content in leaves. Thus, measurements of the optical properties of leaves provide more precise information about changes in the content of chlorophyll than spectrophotometry. Leaves under WBL showed significant differences in reflection within the range 800-1100 nm, which depended on the physiological age of the tissue. For physiologically older tissues, values of reflection were lower than those for physiologically younger tissues. However, under WL conditions, a decrease in reflection within the 800-1100 nm rangę depended only on ozone fumigation. Response of broccoli leaves to spectral composition of irradiation and ozone stress was much higher for irradiation transmission than for reflection. Compared to the control, ozone fumigation of broccoli leaves grown in WBL increased irradiation transmission in OP and IP leaf fragments, both in the 500-700 nm and 750-1100 nm ranges. For plants growing in WL no influence of ozone fumigation on the transmission of irradiation within the visible range was observed. However, fumigation with ozone resulted in a significant decrease in the transmission within the infrared range (750 to 1100 nm). The results indicate the major role of irradiation spectral composition in plant response to ozone stress. The modifications may affect Chl content, leaf temperature and, what is more important, phytochrome-controlled morphogenetic processes.
Genetic maps containing molecular markers are useful tools for the identification of genes underlying quantitative traits (QTLs). Three traits important for plant physiology, i.e. chlorophyll content, sensitivity to GA and sensitivity to ABA, were evaluated for 99 F2 families of the DS2 x RXLl0 rye mapping population. The observed variation in the chlorophyll content in rye leaves was shown to be under the genetic control of four independent QTLs. They were located on the following chromosome arms: 1RL (QChc-1R.I), 3RS (QChc-3R.1), 4RL (QChc-4R.1) and 5RL (QChc-5R.1) The marker-assisted selection of recombinants comprising positively-acting alleles at these loci may significantly increase the chlorophyll content in rye leaves, which should result in a higher efficiency of photosynthesis. A decreasing of plant height in rye is one of the major aims of breeders selecting for lodging resistance. The polymorphism of genes controlling sensitivity to gibberellic acid may be the basis for a potential selection strategy. Three QTLs underlying the sensitivity of rye seedlings to GA were located on chromosomes 5RL (QGar-5R.I), 1RL (QGar-1R.1) and on 7RL (QGar-7R.I). The dwarfing allele of a Dw1 locus present in line RXL10 was mapped in the same position as the QGar-5R.1 gene. Sensitivity to ABA plays an important role in imposing dormancy in rye grain. Three QTLs affecting sensitivity to ABA were found. They were mapped on chromosomes 1RS (QAbr-1R.1), 2R in the centromeric region (QAbr-2R.1) and on 5RL (QAbr-5R.1). QAbr-5R.1, Dw1 and QGar-5R.1 are located in the same position on the distal part of the 5RL chromosome arm, which suggests that they belong to a single locus for a major regulatory gene controlling the development of rye plants.
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The pea seeds were exposed to full-wave rectified sinusoidal magnetic fields. The effects of electromagnetic treatment on seedling growth and chlorophyll contents and have been investigated. Seed were sown after magnetic field treatment according to ISTA under controlled laboratory conditions. The magnetic filed treatment of seeds increased the growth significantly (P<0.05), while the increment in contents of chlorophyll have been found non significant (P<0.05). The shoot length, root length, root dry mass, shoot dry mass, fresh root mass and fresh shoot mass increased up to 140.5, 218.2, and 104, 263.6, 74.5, 91.3%, respectively. The result suggested that magnetic field could be used to enhance the growth in pea plant.
The influence of mycorrhiza with Hebeloma crustuliniforme on plant growth, leaf water potential, leaf gas exchange and chlorophyll content in 3 poplar varieties (P. petrowskyana, P. deltoides cv. Plantierens, P. balsamifera) were investigated. After 11 weeks of plant growth mycorrhizal structures (frequency of mycorrhiza - FF%, intensity of the mycorrhizal colonization - MC% were observed in plant roots belonging to the treatment (M). No statistically significant differences were observed in the frequency of mycorrhiza (F%) and intensity of the mycorrhizal colonization (MC%) in plant roots of the treatment M between the examined poplar cultivars. The frequency of mycorrhiza (FF%) was high, and it reached about 75%, while the intensity of the mycorrhizal colonization (MC%) by fungi hyphae was slightly lower and equaled to from 49 to 58%. For plants, exposed to the inoculation with mycorrhizal fungi, advantageous and statistically significant changes in the measured physiological traits were observed. In this research advantageous differences in reactions between inoculated and non-inoculated poplar cultivars were observed, which was shown in the measurements of some physiological traits. Compared to the control plants (C), in the case of plants of the treatment (M) greater increase in plant height (∆Н), increase in chlorophyll content (SPAD), higher values of water potential (Ψ), and increase - in photosynthesis rate (Pn), stomatal conductance (gs) and water use efficiency (WUE), and decrease in transpiration rate (E), were observed. Although those differences were relatively small, we may presume that if they occur through a longer period they may become more distinct e.g. as a greater increase in height in the inoculated plants.
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