The ultrafiltration of post-culture fluids proved that divercin occurred in the form of large macroaggregates with the molecular weight over 100 kDa. Addition of detergents: Tween 80, Nonidet P-40 and SDS, and alcohols: ethanol and isopropanol into supernatants from post-culture fluids caused partial disaggregation of divercin. The better effect was obtained when Tween 80 was introduced directly into culture medium. However, other detergents and both alcohols showed a toxic effect to the bacteria and reduced the divercin production in C. divergens culture. The ultrafiltration yield of divercin depended on the type of membranes and treatment of divercin-containing extracts with detergents and alcohols. The cellulose acetate membranes were more permeable for divercin that made from polyethersulfone.
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