Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 8

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  cell suspension culture
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
n this work, morphology of Beta vulgaris L. cells permeabilized with 0.7 mM of Triton X-100® was evaluated using digital image processing and concepts of fractal dimension (perimeter- area relations). Important morphometric changes were found when the contact-time with chemical agent was increased.The size of cells decreased, the cells lost the roundness and their shape was more sinuous; this behaviour was a result of a probable shrinkage caused by the excess of exposure with the permeabili- zation agent. Morphology of B. vulgaris cells after permeabili- zation, exhibited a fractal nature since the slope of the ratio of the logarithm of the perimeter vs logarithm of the area was higher than unit. Fractal geometry of the cell morphology was affected as a re- sult of the exposure to Triton X-100®. Those changes can be attri- buted to the loss of turgor and structure of the cell wall.
A simple and rapid procedure for identification and determination of extracellular saccha- rase is described, using a culture medium of Chelidonium majus L. (celandine) cell suspen­sion cultures. Sucrose was used as a substrate for determination of the extracellular and intracellular activities of the studied enzyme. The culture medium (without cells) was used for identification and determination of extracellular enzyme activity. Intracellular activity was estimated using cell suspension.
The establishment of callus and cell suspension cultures of Eleutherococcus senticosus was reported. The four callus lines were obtained from stolon and bud explants from a fieldgrown plant. They were cultured on Murashige and Skoog (MS) or Schenk and Hildebrandt (SH) agar media, supplemented with 30 g/l sucrose, 2,4-dichlorophenoxyacetic acid (2,4-D) (1 mg/l), α-naphtaleneacetic acid (NAA; 1 mg/l) and 6-benzylaminopurine (BAP; 0.5 mg/ l). The cell suspension culture was also maintained in SH liquid medium with the same growth regulators and sucrose (50 g/l). HP LC analysis showed that the cultures produced chlorogenic acid. The highest accumulation of the compound was detected in the cell culture (29.7 mg/g d.w.); it was 15 times the average level reported for starting callus, several times as much as roots, and far more than leaves of intact plants.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.