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1

Changes in peroxidase and IAA oxidase activities during cell elongation in Phaseolus hypocotyls

100%

Bagatharia S. B., Chanda S. V.

Acta Physiologiae Plantarum

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1998

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tom 20

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nr 1

Cytoplasmic and salt-extracted peroxidase and IAA oxidase activities were studied in Phaseolus vulgaris hypocotyls treated with gibberellic acid (GA, 200 µM), naphthyl acetic acid (NAA, 100 µM) and distilled water control (DW). Peroxidase activity was assayed with four hydrogen donors during the initial phase of hypocotyl elongation. Though peroxidase activity showed a decreasing trend with time in all the hydrogen donors studied; considerable variation with different hydrogen donors was observed. NAA had maximum peroxidase activity as compared to DW or GA treatment. The activity showed a clear inverse correlation with hypocotyl growth. IAA oxidase activity showed a similar trend with growth as peroxidase activity. A highly significant correlation was observed between peroxidase and IAA oxidase activities and high molecular weight xyloglucan content (P<0.001). Finally, the possible role of peroxidase and IAA oxidase activities in hypocotyl elongation growth is discussed.

2

Physiological and biochemical changes associated with cotton fibre development. VI. Acid and alkaline phosphatases

100%

Thaker V. S., Saroop S., Vaishnav P. P., Singh Y. D.

Acta Physiologiae Plantarum

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1996

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tom 18

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nr 2

3

Evaluation of effectiveness of the methods for isolation of cell wall polysaccharides during cell elongation in Phaseolus vulgaris seedlings

84%

Chanda S. V.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 3B

Phaseolus vulgaris seedlings were grown in light with or without chromium. Changes in cell wall components i.e. pectic poly tacchatides and xyloglucan contents were looked into during cell elongation, by two different methods in order to find the most suitable method for isolation of cell wall poly saccharides. The first method was short and easy. It made use of organic solvents for preparation of cell wall components and ammonium oxalate and oxalic acid buffer and high temperature for extracting pectic polysaccharides; 0.7 M and 4.3 M KOH was used for extracting low and high molecular weight xyloglucans respectively. On the other hand, in the second method, cell wall components were fractionated by sequential treatments with different inorganic solvents, chelating agents, sodium lauryl sulphate, etc. KOH (1 M and 4 M) was used for extracting xyloglucans. The advantage of ustng the second method for extracting cell wall polysaccharides especially pectic polysaccharides is discussed.

4

Modification of cell wall polysaccharides during cell elongation in Phaseolus vulgaris hypocotyls

84%

Bagatharia S. B., Chanda S. V.

Acta Physiologiae Plantarum

|

1998

|

tom 20

|

nr 1

The effect of gibberellic acid (GA) and naphthylacetic acid (NAA) on hypocotyl elongation and cell wall polysaccharides was studied using Phaseolus vulgaris seedlings grown in light condition. The hypocotyl was demarcated into two segments — one near the root was called lower and the one near the cotyledon was called upper. The upper segment showed a typical sigmoidal growth curve while lower segment did not show any growth at all. GA promoted the growth of upper segment while NAA showed clear inhibition in both the segments. Xyloglucan content showed a clear inverse correlation with growth. Pectic polysaccharides did not show a clear trend, though showed an initial inverse correlation with growth. It is concluded that degradation of low and high molecular weight xyloglucans are involved in cell wall loosening which in turn may be responsible for the elongation growth of Phaseolus hypocotyls in light.

Ograniczanie wyników

4 Acta Physiologiae Plantarum

3 Chanda S. V.

2 Bagatharia S. B.

1 Saroop S.

1 Singh Y. D.

1 Thaker V. S.

1 Vaishnav P. P.

1 2005

2 1998

1 1996

Changes in peroxidase and IAA oxidase activities during cell elongation in Phaseolus hypocotyls

Physiological and biochemical changes associated with cotton fibre development. VI. Acid and alkaline phosphatases

Evaluation of effectiveness of the methods for isolation of cell wall polysaccharides during cell elongation in Phaseolus vulgaris seedlings

Modification of cell wall polysaccharides during cell elongation in Phaseolus vulgaris hypocotyls