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1

Innovative culture techniques of human lens epithelium for cataract research

100%
Webb S. F., Duncan G., Riach R. A.
Folia Histochemica et Cytobiologica. Supplement
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1997
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tom 35
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nr 2
2
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Dietary sources of lutein in adults suffering eye disease (AMD/cataracts)

72%
Sulich A., Hamulka J., Nogal D.
Roczniki Państwowego Zakładu Higieny
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2015
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tom 66
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nr 1
Background. Epidemiological studies indicate that by consuming 6-14 mg lutein daily, the risk of acquiring eye diseases like age-related macular degeneration (AMD) or cataracts becomes reduced. Their symptoms can also by such means be alleviated and treatment improved. Objectives. To estimate dietary intakes of lutein obtained from foodstuffs and supplements along with determining its main sources in selected groups of adults suffering from eye disease and healthy controls. Material and Methods. The study was performed in Warsaw and its neighbourhoods during 2008-12. Subjects were 375 adults aged 50-97 years, of whom half had been diagnosed with AMD and/or cataracts; constituting the test group. Dietary intakes of lutein were assessed by Food Frequency Questionnaire Method whilst interview questionnaires assessed the intake of supplements. Results. Overall, the average dietary intake of lutein from foodstuffs was 2.5 mg daily, with the test group being significantly higher than healthy controls (2.9 vs 2.1 mg daily). Women’s intakes were also higher than in men (2.9 vs 2.1 mg daily), as were those possessing higher or secondary education compared to the others with primary or vocational education (2.7 vs 2.3 mg daily). Fresh vegetables were found to be the main dietary sources of lutein that included green leafy vegetables and frozen vegetables, constituting respectively 63% and 13% of the dietary intake. Dietary supplements containing lutein were taken by 109 subjects of whom most had eye disease (over 80%); where the average daily consumption of lutein from this source was 6.5 mg. Conclusions. For older people, the dietary intake of lutein from foodstuffs may be insufficient to prevent eye disease. Taking daily dietary supplements would thus be indicated to make up such deficiencies of lutein.
3
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Mydrane intracameral injection can be an alternative to mydriatic drops instillation in cataract surgery

72%
Nazim-Lipski G., Bolsega-Pacud J., Kubicka-Trzaska A., Markiewicz A., Romanowska-Dixon B.
Journal of Physiology and Pharmacology
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2020
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tom 71
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nr 2
4

Activity of superoxide dismutase obtained from senile cataract lens - effect of diabetes mellitus

72%
Panz T., Wojcik R., Krukar-Baster K.
Acta Biochimica Polonica
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2008
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tom 55
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nr 4
821-823
The activity of Cu,Zn superoxide dismutase in the fluid obtained from eye lens capsules after cataract surgery was investigated in samples obtained from patients with senile cataract and with senile cataract combined with diabetes mellitus. Two parameters were measured and compared:the frequency of occurrence of detected superoxide dismutase activity and the relative activity of the enzyme in samples derived from senile cataract patients versus those from the patiens affected additionally by diabetes mellitus. It was confirmed that the decrease of superoxide dismutase activity during cataract was additionally promoted by diabetes mellitus.
5

Cathepsin A activity of normal bovine ocular tissues and pathological human intraocular fluids

72%
Obuchowska I., Stankiewicz A., Mariak Z.
Acta Biochimica Polonica
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1996
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tom 43
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nr 4
Cathepsin A activity assayed with N-Cbz-Phe-Ala, N-Cbz-Glu-Tyr and N-Cbz- -Glu-Phe as substrates, was measured in fresh corneas, lenses, aqueous humor, vitreous humor and choroid plus retinal pigment epithelium taken from normal bovine eye balls and in human intraocular fluids from the eye balls in various ocular diseases (cataract, glaucoma, diabetes, intraocular tumors). Cathepsin A exhibited a pH optimum at 5.0 and showed the highest specificity towards N-Cbz-Phe-Ala as a substrate. In bovine ocular tissues high cathepsin A activity was found in the choroid plus retinal pigment epithelium and in cornea. The lens and the vitreous humor showed low enzyme activity and the aqueous humor none at all. In the human aqueous humor of the eye with cataract cathepsin A activity was more than three times higher then in the eye with choroid tumor. In human vitreous humor in absolute glaucoma the activity was twice as high as in melanoma and almost three times higher than in the case of lung metastatic tumor. Diabetes in glaucoma increased seven fold cathepsin A activity in the vitreous humor.
6

Comparing the content of lipids derived from the eye lenses of various species

58%
Panz T., Lepiarczyk M., Zuber A.
Folia Histochemica et Cytobiologica
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2011
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tom 49
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nr 3
7

Implication of glycolipids in lens fiber development

58%
Ogiso M.
Acta Biochimica Polonica
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1998
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tom 45
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nr 2
Mammalian lens contains Lewisx, sialyl-Lewisx and α-galactosyl epitopes in neolacto-series glycosphingolipids. The expression of these three epitopes is not observed in lens epithelial cells, but is immunohistochemically detected in the inner cortical fibers and the lens nucleus. In embryonic chick lens, sialyl-Lewisx-containing gangliosides were also detected in the transitional zone and elongating lens fibers. Thus, the Lewisx, sialyl-Lewisx and α- galactosyl epitopes may be associated with the differentiation and maturation of lens epithelial cells to lens fibers.
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