Wyniki wyszukiwania

1

Effect of sex and localization dependent differences of Na,K-ATPase properties in brain of rats

100%

Kolocayova B., Vrbjar N.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 2

2

Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China

100%

Wei F., Wang C., Wang Z., Kong Y., Shi Y., Shi J., Qi D., Qi H., Liang L., Zhang R., Li C.

Folia Histochemica et Cytobiologica

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2014

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tom 52

|

nr 3

3

Rational design of new competitive inhibitors of human CK2 activity

100%

Moro S., Ben D. D., Zagotto G., Meggio F., Sarno S., Pinna L. A.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2A

4

A homolog of catalytic subunit of CK2alpha in maize

100%

Lebska M., Szczegielniak J., Gnatowska E., Moro S., Pinna L. A., Dobrowolska G., Muszynska G.

Biological Letters

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2005

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tom 42

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nr 2 Spec.Vol.

5

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

80%

Trapp-Fragnet L., Marie-Egyptienne D. T., Fakhoury J., Rasschaert D., Autexier C.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 4

The minimal vertebrate telomerase enzyme is composed of a protein component (telomerase reverse transcriptase, TERT) and an RNA component (telomerase RNA, TR). Expression of these two subunits is sufficient to reconstitute telomerase activity in vitro, while the formation of a holoenzyme comprising telomerase-associated proteins is necessary for proper telomere length maintenance. Previous reports demonstrated the high processivity of the human telomerase complex and the interspecies compatibility of human TERT (hTERT). In this study, we tested the function of the only known viral telomerase RNA subunit (vTR) in association with human telomerase, both in a cell-free system and in human cells. When vTR is assembled with hTERT in a cell-free environment, it is able to interact with hTERT and to reconstitute telomerase activity. However, in human cells, vTR does not reconstitute telomerase activity and could not be detected in the human telomerase complex, suggesting that vTR is not able to interact properly with the proteins constituting the human telomerase holoenzyme.

6

Interaction between plant polyphenols and the erythrocyte membrane

70%

Cyboran S., Oszmianski J., Kleszczynska H.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 1

The purpose of these studies was to determine the effect of polyphenols contained in extracts from apple, strawberry and blackcurrant on the properties of the erythrocyte membrane, treated as a model of the biological membrane. To this end, the effect of the substances used on hemolysis, osmotic resistance and shape of erythrocytes, and on packing order in the hydrophilic region of the erythrocyte membrane was studied. The investigation was performed with spectrophotometric and fluorimetric methods, and using the optical microscope. The hemolytic studies have shown that the extracts do not induce hemolysis at the concentrations used. The results obtained from the spectrophotometric measurements of osmotic resistance of erythrocytes showed that the polyphenols contained in the extracts cause an increase in the resistance, rendering them less prone to hemolysis in hypotonic solutions of sodium chloride. The fluorimetric studies indicate that the used substances cause a decrease of packing order in the hydrophilic area of membrane lipids. The observations of erythrocyte shapes in a biological optical microscope have shown that, as a result of the substances’ action, the erythrocytes become mostly echinocytes, which means that the polyphenols of the extracts localize in the outer lipid monolayer of the erythrocyte membrane. The results obtained indicate that, in the concentration range used, the plant extracts are incorporated into the hydrophilic area of the membrane, modifying its properties.

Ograniczanie wyników

Effect of sex and localization dependent differences of Na,K-ATPase properties in brain of rats

Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China

Rational design of new competitive inhibitors of human CK2 activity

A homolog of catalytic subunit of CK2alpha in maize

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

Interaction between plant polyphenols and the erythrocyte membrane