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The Avena genus covers nine species in Poland, including farmed common oat (Avena sativa), wild oat (A. fatua) – a dangerous spring cereal weed, and bristle (or black) oat (A. strigosa Schreb.), a forgotten species. Bristle oat was a valuable component of common oat yield growing on the weakest soils, and it had a status of a crop plant in Poland and in many European countries till 1950s. Chemical analyses of bristle oat caryopses validated the high nutritive value of this species, which had been previously noted by the farmers of the Podhale region. On average, bristle oat contains 27–52% more protein, 14–27% more fat and 38–72% more sugars than common oat. It is good for human consumption in the form of flakes, flour and boiled grains. Bristle oat is witnessing a revival as a valuable farming species, and its crops are subsidized.
During the development and ripening of triticale caryopses cv. Grado, a gradual increase in the precocious germination ability of the grain was observed. After 48 hrs of incubation (at 22 °C under optimal moisture conditions for germination in darkness) of the freshly-collected caryopses of different ripeness, the percentage of polysomes in the ribosomal fraction isolated from embryonic tissue in buffer A + PTE (to yield released FP + MBP) was about 15 % in the sample of grains gathered at the milk-ripeness stage, over 27 % at the wax-ripeness stage and over 63 % at the full-ripeness stage. Percentages of polysomes solubilized in the pellets in buffer U (TBP, putative cytoskeleton-bound polysomes), were always higher and amounted to about 36 % at the milk-ripeness stage, 58 % at the wax-stage and 68 % at the full-ripeness stage. The incorporation of 3H-uridine into polysomal RNA (mRNA+rRNA) of triticale embryos increased during the development and maturation of the caryopses subjected to germination from about 19 and 33 Bq/mg RNA at the milk-ripeness in the FP+MBP and the TBP to 220 and 312 Bq·mg⁻¹ RNA at the full-ripeness stage, respectively. The effect of the pericarp and other tissues surrounding the embryo was also investigated. Removal of the outer pericarp strongly stimulated germination of unripe caryopses in the middle period of development e.g. 32 and 39 DAF. The strongest stimulatory effect on transcription was found in the embryo during germination, in all samples of caryopses of various degrees of ripeness, by isolating it completely from the pericarp, testa and endosperm. The high percentage of polysomes in the TBP and the high incorporation 3H-uridine into RNA in that fraction during precocious germination suggests an important role for this sub-population of polysome (and the proteins synthesised by them) in initiation of precocious germination processes.
This study was conducted on barley cv. Ars. caryopses collected at full ripeness and divided into two batches. From one batch (dormant caryopses) polysomes were isolated from embryos immediately after harvesting and after two days of germination. From the other batch (non-dormant caryopses) the same was done after eight months storage in a dry state. A low ionic strength cytoskeleton-stabilizing buffer was used for the isolation of polysomes. Four different fractions of polysomes were examined: free polysomes (FP), membrane-bound polysomes (MBP), cytoskeleton-bound polysomes (CBP) and cytoskeleton-membrane-bound polysomes (CMBP). In germs grown from non-dormant caryopses, the first two fractions (FP + MBP) made up about 78 % of the total ribosomal material, whereas in embryos of dormant, imbibed caryopses, two last fractions (CBP + CMBP) made up about 71 %. The percentage of polysomes after 48 hours of imbibition of dormant caryopses in the FP, MBP and CBP was only about 13 % (i.e., 87 % monosomes), whereas a greater proportion (19.4 %) was found in the CMBP. The highest incorporation of ³H-uridine and ¹⁴C-amino acids (after 48 hours of germination and 0.5, 3 and 6 hrs incubation with precursors) took place in trhc CMBP both in dormant and non-dormant caryopses The major amount of the two polysome fractions associated with the cytoskeleton (CBP and CMBP) and the higher activity of CMBP in protein synthesis in embryos of dormant, imbibed triticale caryopses may indicate a significant role for polysomes associated with the cytoskeleton in the control of protein synthesis in dormant and germinating caryopses.
Some posttranslational processes that occur in embryos of germinating triticale caryopses treated with different concentrations of abscisic acid (ABA) were examined. ABA increased the ratio of cytoskeleton-bound polysomes in the total population of polysomes and depressed the share of free and membrane-bound polysomes. Using exogenous RNase, stability of the total polysomal population as well as each polysomal fraction was investigated. The total extractable polysomes isolated from embryonic tissues of germinating triticale caryopses treated with ABA were more stable than the polysomes isolated from the control sample caryopses. The contribution of the polysomes that were not digested by RNase was increased by higher concentrations of ABA applied during germination. At high concentrations of ABA (50, 100 pM), the quantitative contribution of polysomes in the total ribosomal fraction was almost 100 % ofthe amount ofpolysomes before digestion and the modifications observed consisted mainly of the shift of the socalled heavy polysomes towards light polysomes, containing a few ribosomes. Within each polysomal population, cytoskeleton-bound polysomes (CBP and CMBP) were the most stable, which may imply that the bonds between polysomes and these protein filaments, created in all eukaryotic cells increased their stability. It is assumed that mRNAs are stabilised or destabilised by interaction of proteins with their various sequences. A plant hormone may depress or elevate the quantities of these proteins, thus regulating the stability of different mRNAs. The results confirm the multi-faceted mechanism of ABA-induced response, where one of the constituents is the effect ofABA on the stability ofmRNAs molecules. The coordinated regulation ofmRNAs synthesis and their stability provide plants with improved adaptability.
Badano zabarwienie ziarna pod wpływem fenolu i zabarwienie koleoptyla trzech kolejnych generacji pszenżyta ozimego odmian Bogo i Presto reprodukowanych w trzech punktach badawczych (Lublin, Olsztyn, Sandomierz). Doświadczenie prowadzono w latach 1997–2000 z zachowaniem izolacji przestrzennej. Jako kontrolę wysiewano co roku materiał wyjściowy z przechowalni długoterminowej. Nie stwierdzono istotnych różnic pomiędzy kontrolą i reprodukcją dla obu cech, ale zaznaczyły się różnice pomiędzy latami zbioru. Badany materiał odmiany Bogo i odmiany Presto nie różnił się od wzorców pod względem reakcji fenolazowej i zabarwienia koleoptyla, jedynie zabarwienie koleoptyla u odmiany Presto było nieco słabsze niż wzorca.
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