Wyniki wyszukiwania

1

Cross-linking of nuclear proteins to DNA by platinum compounds

100%

Walter Z., Wozniak K., Kowalik J.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 4

In this work we investigated the lymphocyte nuclear proteins which participated in DNA-protein cross-links induced by three platinum compounds: cis-DDP, trans-DDP and carboplatin. Our studies indicated that many proteins were cross- linked to DNA in the intact cell. Trans-DDP was the most effective cross-linker. The cross-linking process depended on time of the cells incubation with aforementioned compounds. Carboplatin produced the DNA-protein cross-links more slowly and less effectively than cis-DDP. After incubation of the cells with cis-DDP, trans- DDP and carboplatin a similar protein pattern was obtained. In case of all compounds studied 34.5 kDa protein was abundantly represented.

2

Differences in the inhibition of neuritic outgrowth in organotypic cultures of rat foetal dorsal root ganglia treated with cisplatin and carboplatin: a comparative study

84%

Jirsova K., Mandys V.

Folia Histochemica et Cytobiologica

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1997

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tom 35

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nr 4

3

Dicarboxylate platinum [II] complexes as inhibitors of plasma membrane Hplus-ATPase in the yeast Saccharomyces cerevisiae

67%

Oblak E., Lachowicz T. M., Waszkiewicz K., Kuduk-Jaworska J.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 4

A series of cytotoxic neutral dicarboxylatoplatinum(II) complexes containing D(+), L(-) or DL-malate dianion and ethylenediamine or 1-ethylimidazole as ligands were examined using ATPase activity assays and the proton extrusion test. ATPase activity assays in vitro on plasma membrane H+- ATPase and on mitochondrial ATPase were carried out. The concentrations of compounds inhibiting enzyme activity to 50 per cent (J50) was determined. The new platinum complexes showed a stronger level of inhibition of both ATPases than the reference carboplatin; this inhibitory activity is related to a stereoisomeric form of anionic platinum ligands. ATPase inhibition in vivo was tested by glucose-stimulated proton extrusion and the influence of platinum compounds on this process in yeast cells was determined. Significant differences in activity levels were observed between those complexes with 1-ethylimidazole and those with ethylenediamine.

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

1 Folia Histochemica et Cytobiologica

1 Jirsova K.

1 Kowalik J.

1 Kuduk-Jaworska J.

1 Lachowicz T. M.

1 Mandys V.

1 Oblak E.

1 Walter Z.

1 Waszkiewicz K.

1 Wozniak K.

1 2003

2 1997

Cross-linking of nuclear proteins to DNA by platinum compounds

Differences in the inhibition of neuritic outgrowth in organotypic cultures of rat foetal dorsal root ganglia treated with cisplatin and carboplatin: a comparative study

Dicarboxylate platinum [II] complexes as inhibitors of plasma membrane Hplus-ATPase in the yeast Saccharomyces cerevisiae