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Studies were carried out on the utilization of various carbohydrates by planktonie bacteria isolated from the estuarine lakes Łebsko, Gardno and Jamno. The most actively utilized carbohydrates were glucose, fructose, lactose and saccharose; the least active was rhamnose. Pronounced seasonal variability in the utilization of carbohydrates was found. However, no significant differences were found at the particular research stations.
Aim of this study was to elucidate the influence of carbohydrate store modification on exercise capacity and catecholamine thresholds. Nine sedentary students and 10 endurance athletes volunteered for the study consisting of four sessions in one-week intervals. During control session (C) subjects performed graded exercise till exhaustion with workload increasing by 50 Watts every 3 min after an overnight fast. Blood lactate and catecholamines were determined at rest, after each workload and at exhaustion. In the evening preceding each of the remaining three sessions subjects performed glycogen reducing exercise lasting 1.5 hrs at 70% HRmax. Till next morning they did not eat any meal but drank water. Two hours before exercise subjects were given either a high-carbohydrate (H-CHO) or a low-carbohydrate (L-CHO) meal of approx. 1000 kcal or remained fasted (F). Depletion of carbohydrate stores enhanced contribution of fat-derived energy substrates at submaximal workloads, but did not influence either maximal oxygen consumption, workload and lactate concentration or lactate threshold. Low carbohydrate availability resulted in elevated concentration of catecholamines only in untrained subjects. Ingestion of a meal either high or low in carbohydrates diminished those changes. Catecholamine thresholds were similar in all sessions and higher in athletes’ group.
The mesh size of sieves has a significant impact upon soil disturbance, affecting pore structure, fungal hyphae, proportion of fungi to bacteria, and organic matter fractions. The effects are dependent upon soil type and plant coverage. Sieving through a 2 mm mesh increases mineralization of exogenously supplied carbohydrates and phenolics compared to a 5 mm mesh and the effect is significant (p<0.05), especially in organic horizons, due to increased microbial metabolism and alteration of other soil properties. Finer mesh size particularly increases arabinose, mannose, galactose, ferulic and pthalic acid metabolism, whereas maltose mineralization is less affected. Sieving through a 5 mm mesh size is suggested for all type of experiments where enhanced mineralization of low-molecular-weight organic compounds needs to be minimalized.
Tomato plants were cultivated (from 2 to 23 days after germination) in media with NO3-, NH+, or a mixture of both forms in different proportions used as the N source given with or without 5 mol dm-3 HCO3-. The accumulation of soluble sugars (reductng sugars and sucrose) and free amino acids was higher in the roots and leaves of NH+-fed plants than in NO3--fed plants. Starch accumulation in NH4+-fed plants was higher in leaves (about 28 %) and lower in roots (about 37 %) in comparison with that of NO3-- fed plants. Plants cultivated in media containing a mixture of NO3-/NH4+ were characterized by a lower content of sugars and amino acids accumulation in comparison with that in plants fed with NO3- or NH4+. An elevated HCO3- concentration in the rhizosphere stimulated the accumulation of soluble sugars and free amino acids in all the experimental variants. There were only small differences in the starch content.
Seed sculpture, seed coat thickness and soluble carbohydrate profiles were compared in three lupin species, each represented by three different genetic lines, from sections Pilosus (L. pilosus) and Atlanticus (L. atlanticus, L. cosentinii). Seed coat thickness varied most in the three lines of L. pilosus (330-1000 µm), while in the other species it varied little from 400 µm thickness. The studied seeds accumulated approximately 100 mg/g d.m. soluble carbohydrates. The obtained results together with published data suggest that the soluble carbohydrate composition of seeds is clearly section-specific in Lupinus sections Pilosus and Atlanticus. Sect. Pilosus was characterized by a high level of galactosyl cyclitols, while seeds of sect. Atlanticus contained much higher amounts of the raffinose-family oligosaccharides. In all species, however, the dominating soluble carbohydrate was stachyose. The results on the biochemical parameters analyzed in this paper confirm that L. atlanticus and L. cosentinii are more closely related to each other than to L. pilosus. The seed coat roughness/smoothness criterion seems inadequate for building a classification of lupin species, as one of the analyzed lines of L. pilosus, formally ascribed to the rough-seeded lupins, produces smooth seeds.
Nodal segements were taken from juvenile shoots of mature 100 year-old trees of saucer magnolia (Magnolia x soulangiana Soul.-Bod.) and cultured on Standardi and Catalano medium supplemented with 1.33 µmol·dm⁻³ BA, 0.54 µmol·dm⁻³ NAA, 58 µmol·dm⁻³ sucrose and 6.0 g·l⁻¹ agar-agar. After 8 weeks, separated shoots were transferred to rooting medium with half-strength macronutrients (basal medium) supplemented with 0.3% activated charcoal and one of carbohydrates: arabinose, cellulose, fructose, galactose, glucose, lactose, mannose, rhamnose, ribose, sorbose, sucrose or xylose at 20 g·dm⁻³ and 7.0 g·dm⁻³ agar-agar. After 13 weeks of culture, shoot number, fresh and dry weight of shoots and roots, total root length and number of roots/per shoot were recorded. Percentages of rooted shoots were calculated. Fructose, mannose and xylose were the most effective carbon source on shoot proliferation followed by sucrose. The rooting response was induced by cellulose and xylose. Arabinose, rhamnose and sorbose inhibited root formation. The number of adventitious roots produced per shoot was stimulated by cellulose and xylose. Total biomass (shoot plus roots) of the plantlets was the highest at fructose and cellulose.
Flower buds, cotyledons and hypocotyls of Pharbitis nil were used as plant material. Flower buds (1-2 mm long) were excised from 3-week-old plants, grown in soil. Cotyledons of 7-day-old sterile seedlings were cut into 25 mm2 squares cotyledons whereas hypocotyls were cut to 1 mm long fragments. Explants were transferred into Petri dishes containing the Murashige and Skoog medium (MS), supplemented with either BA (11 μM·U⁻¹) alone or BA (22 μM·U⁻¹) and NAA (0.55 μM·U⁻¹), and different sugars: sucrose, fructose, glucose, mannose or sorbitol (autoclaved or filter-sterilized). Addition of glucose instead of sucrose to the medium stimulated the induclion of callus on flower buds and cotyledoniry explants, but inhibited its growth on fragments of hypocotyls. The medium supplemented with fructose (especially filter-sterilized) stimulated the development of flower elements. Organogenesis of shoots and roots on explants was also observed. Flower buds and hypocotyls were able to regenerate both organs. Addition of fructose or glucose to the medium stimul ated the organogenesis of shoots, whereas root organogenesis was inhibited on all explants used. Sorbitol strongly inhibited both induction of callus and organogenesis on all explants used.
Germinability and the content of soluble carbohydrates were analysed in cereal seed (winter rye, cv. Warko; spring wheat, cv. Santa; hexaploid winter triticale, cv. Fidelio and cv. Woltario). Seed moisture content (mc) was equilibrated over silica gel to 0.08 g H2O/g dry mass and stored in a desiccator at 20oC for up to 205 weeks or were equilibrated to mc 0.06, 0.08 or 0.10 g H2O/g dm and subjected to artificial aging at 35oC in air-tight laminated aluminium foil packages for 205 weeks. It was shown that the rate of seed aging depended on the species and seed moisture content. The fastest decrease of germinability upon storage was observed in seed with the highest moisture level. Complete germinability loss for winter rye, winter triticale cv. Fidelio, winter triticale cv. Woltario and spring wheat seed with mc 0.10 g H2O/g dm3 occurred after 81, 81, 101 and 133 weeks, respectively. Fructose, glucose, galactose, myo-inositol, sucrose, galactinol, raffinose, stachyose and verbascose were the main soluble carbohydrates found in the seed. The obtained data on the contents of specific sugars and the composition of soluble sugars fraction in seed of rye, wheat and triticale did not corroborate any profound effect of reducing sugars, sucrose and oligosaccharides on seed longevity.
A field experiment was carried out at the Research Station of Eghlid-Azad University Iran in two years (2007 and 2008), based on a randomized complete block design with three replications. Three saline irrigation water treatments: 1 control, 6 and 12 dS·m-1 were arranged as main plots and three hull-less barley cultivar (U46M, GIZA 129 and PV110) were used as subplots. The measured parameters were growth, yield components, glucose, starch and sucrose content, relative water content and photosynthetic parameters in flag leaves. The results indicated that the number of ear and grain per plant were reduced significantly by stress. Grain weight was less sensitive to stress. Biological and grain yields were decreased by stresses. Among the cv. U46M had the lowest and PV110 the highest grain and biological yield. Biological yield differences were related to lower plant height and tillers. The grain yield reduction was related to ear no. per plant and grain no. per ear reductions. Salinity stress caused reduction in starch and increased sucrose contents. There were no significant differences between cultivars in glucose content. There were high negative correlation between sucrose content and grain yield and positive correlation between grain yield and starch content.
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