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Construction and immunogenic properties of DNA vaccine against canine parvovirus infection

100%

Mizak B., Rzezutka A., Krol J., Kazula A.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 2

The presented studies were concentrated on construction of the recombinant mammalian expression vector - pSecTag2B, carrying canine parvovirus VP2 gene and its N-terminal fragment. Evaluation of safety of experimental vaccines was done on laboratory animals and immunogenic properties were tested on dogs. CPV specific antibodies were not detected by HI test in serum samples collected from dogs vaccinated with recombinant plasmid pSecTag2B-CPV582. The presence of CPV specific antibodies (80-320 by HI) was confirmed 10 days after vaccination in all dogs vaccinated with pSecTag2B-CPV1774. The highest titre was obtained in sera collected from dogs vaccinated with 200 μg and 300 μg of DNA. Fourteen days after revaccination, the level of antibodies increased up to 160 in dogs vaccinated with 100 μg of DNA and up to 640 in puppies vaccinated with 200 μg and 300 μg of DNA. The antibodies were detected for at least 16 weeks. Results of the studies demonstrated the immunogenic properties of elaborated DNA vaccine.

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Molecular characterisation and genetic diversity of canine parvovirus type 2 prevalent in Central China

84%

Hu W., Xu X., Liu Q., Ji J., Kan Y., Yao L., Bi Y., Xie Q.

Journal of Veterinary Research

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2020

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tom 64

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nr 3

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Application of nested PCR for the detection of canine parvovirus in faeces

84%

Mizak B., Rzezutka A.

Bulletin of the Veterinary Institute in Puławy

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1999

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tom 43

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nr 1

In our studies two primer pairs within the VP2 protein gene of canine parvovirus were chosen on the base of the sequence of Polish isolates. The sensitivity of detection of canine parvovirus in stool samples by Nested PCR was increased 60% in comparison with the standard PCR method and it was 30% higher than the virus isolation in tissue culture. A simple procedure of sample preparation with the use of Chelating resin was used and it made the Nested PCR easily applicable in rapid confirmation of the diagnosis of parvovirus infection.

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Isolation of Polish strains of blue fox parvovirus [BFPV]

84%

Mizak B.

Bulletin of the Veterinary Institute in Puławy

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1994

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tom 38

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nr 2

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Canine parvovirus and enterotoxigenic Escherichia coli causing the death of a puppy in a kennel

84%

Rzezutka A., Osek J., Mizak B.

Bulletin of the Veterinary Institute in Puławy

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2003

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tom 47

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nr 2

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Antigenic typing polish isolates of canine parvovirus

84%

Mizak B., Plucienniczak A.

Bulletin of the Veterinary Institute in Puławy

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1995

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tom 39

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nr 2

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Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in Central and Eastern China during 2018–2019

67%

Hu W., Zheng L., Xu X., Liu Q., Ji J., Yao L., Kan Y., Bi Y.

Journal of Veterinary Research

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2020

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tom 64

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nr 4

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Detection of canine parvovirus by polymerase chain reaction

67%

Mizak B., Rzezutka A.

Bulletin of the Veterinary Institute in Puławy

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1997

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tom 41

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nr 2

The polymerase chain reaction (PCR) was used to demonstrate the presence of CPV DNA in tissue cultures and faecal suspensions. A simple procedure of sample preparation was elaborated and it made the PCR easily applicable in rapid confirmation of the diagnosis of parvovirus infection. The results of PCR were compared with virus isolation, haemagglutination and immunoenzymatic tests.

Ograniczanie wyników

6 Bulletin of the Veterinary Institute in Puławy

2 Journal of Veterinary Research

6 Mizak B.

4 Rzezutka A.

2 Bi Y.

2 Hu W.

2 Ji J.

2 Kan Y.

2 Liu Q.

2 Xu X.

2 Yao L.

1 Kazula A.

1 Krol J.

1 Osek J.

1 Plucienniczak A.

1 Xie Q.

1 Zheng L.

2 2020

1 2003

1 2001

1 1999

1 1997

1 1995

1 1994

Construction and immunogenic properties of DNA vaccine against canine parvovirus infection

Molecular characterisation and genetic diversity of canine parvovirus type 2 prevalent in Central China

Application of nested PCR for the detection of canine parvovirus in faeces

Isolation of Polish strains of blue fox parvovirus [BFPV]

Canine parvovirus and enterotoxigenic Escherichia coli causing the death of a puppy in a kennel

Antigenic typing polish isolates of canine parvovirus

Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in Central and Eastern China during 2018–2019

Detection of canine parvovirus by polymerase chain reaction