Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 14

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  cancer tissue
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Reference genes for gene expression studies on non-small cell lung cancer

100%
Gresner P., Gromadzinska J., Wasowicz W.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 2
307-316
Study Objective: The aim of this study was to test a panel of 6 reference genes in order to identify and validate the most suitable reference genes for expression studies in paired healthy and non-small cell lung cancer tissues. Method: Quantitative real-time PCR followed by the NormFinder- and geNorm-based analysis was employed. The study involved 21 non-small cell lung cancer patients. Results: The analysis of experimental data revealed HPRT1 as the most stable gene followed by RPLP0 and ESD. In contrast, GAPDH was found to be the least stable gene. HPRT1 together with ESD was revealed as the pair of genes introducing the least systematic error into data normalization. Validation by bootstrap random sampling technique and by normalizing exemplary gene expression data confirmed the results. Conclusion: Although HPRT1 and ESD may by recommended for data normalization in gene expression studies on non-small cell lung cancer, the suitability of selected reference genes must be unconditionally validated prior to each study.
2

Localization of human chorionic gonadotropin beta subunit transcripts in ovarian cancer tissue

100%
Nowak-Markwitz E., Jankowska A., Szczerba A., Andrusiewicz M., Warchol J. B.
Folia Histochemica et Cytobiologica
|
2004
|
tom 42
|
nr 2
3

Inhibition of cathepsin B acivity in human breast cancer tissue by cysteine peptidase inhibitor isolated from human placenta: immunohistochemical and biochemical studies

100%
Saleh Y., Siewinski M., Sebzda T., Jelen M., Ziolkowski P., Gutowicz J., Grybos M., Pawelec M.
Folia Histochemica et Cytobiologica
|
2003
|
tom 41
|
nr 3
4

Specific expression of TNFalpha and TNF-RII receptors in human colon cancer tissues

100%
Muc-Wierzgon M., Mazurek U., Nowakowska-Zajdel E., Kokot T., Zubelewicz-Szkodzinska B., Cholewa K., Wierzgon J., Sosada K.
Acta Biologica Cracoviensia. Series Zoologia
|
2004
|
tom 46
5

Vascular endothelial growth factor [VEGF]-C - a potent risk factor in children diagnosed with stadium 4 neuroblastoma

84%
Nowicki M., Konwerska A., Ostalska-Nowicka D., Derwich K., Miskowiak B., Kondraciuk B., Samulak D., Witt M.
Folia Histochemica et Cytobiologica
|
2008
|
tom 46
|
nr 4
493-499
6

Changes of the expression of Lewis blood group antigens in glycoproteins of renal cancer tissues

84%
Borzym-Kluczyk M., Radziejewska I.
Acta Biochimica Polonica
|
2013
|
tom 60
|
nr 2
 Sialic acid and sialyl Lewisa/x are found on N- and O-glycans of many human malignant cells. Carbohydrate antigens can be used as tumor markers, and an increase of their levels in cancer cells is associated with tumor progression. The aim of this study was to assess the level of some Lewis blood group antigens on glycoproteins in tumor (cancer tissue), intermediate zone (adjacent to tumor tissue), and normal renal cortex/medulla (uninvolved by tumor). The study was performed on tissues taken from 30 patients. Relative amounts of sugar structures of proteins with molecular masses above 30 kDa were determined by ELISA-like test with biotinylated lectins: MAA (Maackia amurensis), SNA (Sambucus nigra), and monoclonal antibodies anti-sialyl Lewisa/x. Higher expression of all examined structures was revealed in cancer tissues. Significant increases were observed for sialic acid linked α 2-3 in cancer tissues when compared to healthy ones and also among intermediate and healthy tissues. The sialic acid linked α 2-6 and sialyl Lewisx structures were significantly increased in cancerous cells when compared to normal and intermediate renal tissue. In case of sialyl Lewisa antigen, a significant difference was discovered between normal and intermediate tissue. Our results confirm that the examined Lewis antigens can be involved in tumor development. Their increase in cancer tissues can suggest their specific role in the process.
7

Molecular markers [c-erbB-2, p53] in breast cancer

84%
Agrawal A. K., Jelen M., Rudnicki J., Grzebieniak Z., Zukrowski P., Nienartowicz E.
Folia Histochemica et Cytobiologica
|
2008
|
tom 46
|
nr 4
449-455
8

p53-dependent suppression of the human calcyclin gene [S100A6]: the role of Sp1 and of NFkappaB

84%
Kroliczak W., Pietrzak M., Puzianowska-Kuznicka M.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 3
559-570
Calcyclin (S100A6) is believed to participate in cell cycle control. It was, however, unclear if its expression depends on p53, a key regulator of apoptosis and cell cycle. We therefore performed transcription regulation assays in HeLa cells and found that wild type p53 suppressed the S100A6 promoter up to 12-fold in a dose-dependent manner. In contrast, the well-characterized V143A, R175H, R249S, and L344A p53 mutants cloned from human cancers suppressed this promoter with a 6 to 9-fold lower efficiency. All the sites mediating the p53-dependent suppression were contained in the –167 to +134 fragment of the S100A6 promoter. Separate overexpression of either Sp1 or of NFκB only partially counteracted the p53 inhibitory effect on the S100A6 promoter, while simultaneous overexpression of both these transactivators resulted in a complete abolishment of the p53 inhibitory effect on this promoter. Sp1 and NFκB binding to the probes resembling their putative binding sites present in the S100A6 promoter was decreased in the presence of wild type p53. We propose that the suppression of S100A6 is yet another mechanism by which p53 inhibits proliferation. Insufficient suppression of this gene by p53 mutants could well be responsible for calcyclin overexpression and cell cycle deregulation observed in cancer tissues.
9

The anti-apoptotic protein lifeguard is expressed in breast cancer cells and tissues

84%
Bucan V., Reimers K., Choi C. Y., Eddy M.-T., Vogt P. M.
Cellular and Molecular Biology Letters
|
2010
|
tom 15
|
nr 2
Lifeguard (LFG) is an anti-apoptotic protein that inhibits Fas-mediated death in tumour cells. However, the molecular function of human LFG in the carcinogenesis of human breast cells is uncertain. We studied the expression and function of endogenous LFG in four breast cancer cell lines (MCF-7, MDA-MB-231, T-47D and HS 578T), a human breast epithelial cell line (HS 578Bst), and in healthy and cancerous breast tissues. Molecular (Western blot and RT-PCR) and immunohistochemical techniques were used to investigate the LFG expression. To investigate the breast cancer cell proliferation in the presence of Fas, we performed fluorescent cell viability assays. The possible association of Fas with LFG was analyzed by immunofluorescence microscopy. In this paper, we provide convincing evidence that LFG is overexpressed in several human breast cancer cell lines. More importantly, we found that the LFG expression correlates with high tumour grades in primary breast tumours. Finally, we demonstrated that Fas sensitivity is reduced in breast cancer cell lines expressing LFG. Our results indicated that LFG is strongly expressed in breast cancer epithelial cells. Moreover, the overexpression of LFG correlated with tumour grade and reduced Fas sensitivity. Our findings support the idea that LFG may have a role in the downregulation of apoptosis in breast cancer cells.
10

Analysis of expression of AIF and PARP-1 in cancerogenesis of HPV-positive cervix

67%
Warowicka A., Kwasniewska A., Sobieraj M., Gozdzicka-Jozefiak A.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 3
The mRNA expression of AIF and PARP-1 in HPV negative non-tumour epithelial cells and HPV-positive cervical cancer cells using the real-time PCR method was examined. An increased level of AIF and PARP-1 mRNA in cervical cancer cells in comparison to normal epithelial cells was demonstrated. These results suggest that changes in the mRNA expression level of AIF and PARP-1 might be involved in cervical cancer development. The analysis of these two factors may represent a new molecular tool for cervical cancer prevention in women with HPV persistent infection.
11

A multiple marker analysis of apoptosis-associated protein expression in non-small cell lung cancer in a Chinese population

67%
Fan C.-F., Xu H.-T., Lin X.-Y., Yu J.-H., Wang E.-H.
Folia Histochemica et Cytobiologica
|
2011
|
tom 49
|
nr 2
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

GABA content and GAD activity in colon tumors taken from patients with colon cancer or from xenografted human colon cancer cells growing as s.c. tumors in athymic nu-nu mice

59%
Kleinrok Z., Matuszek M., Jesipowicz J., Matuszek B., Opolski A., Radzikowski C.
Journal of Physiology and Pharmacology
|
1998
|
tom 49
|
nr 2
13

Poziomy biologiczne kadmu, cynku, miedzi i selenu u pacjentow z nowotworem jelita grubego

43%
Darago A., Rzetecki T., Dziki A., Sapota A.
Bromatologia i Chemia Toksykologiczna
|
2005
|
tom 38
|
nr 4
371-376
W pracy oznaczono poziom kadmu, selenu, miedzi i cynku w wycinkach pooperacyjnych tkanki zdrowej i nowotworowej jelita grubego techniką AAS. Z przeprowadzonych badań wynika, ze zawartość miedzi, selenu i cynku w tkance nowotworowej była wyższa niż w tkance zdrowej, natomiast poziom kadmu był nieznacznie niższy. Wysoki stopień korelacji bliski 1 w tkance zdrowej stwierdzono dla cynku i miedzi. W tkance nowotworowej korelacja między tymi pierwiastkami była znacznie niższa (ok. 0,7) co może wskazywać na zaburzenie homeostazy oznaczanych pierwiastków.
14

Porownanie zawartosci wybranych pierwiastkow w tkankach nowotworowych i prawidlowych u szczurow z wyindukowanym DMBA nowotworem sutka

34%
Skrajnowska D., Polec A., Tokarz A.
Bromatologia i Chemia Toksykologiczna
|
2008
|
tom 41
|
nr 3
484-488
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.