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The role of external Ca2plus in the action of Ca2plus-channel agonists and antagonists on isolated human thoracic arteries

100%

Garaliene V., Barsys V., Giedraitis S., Benetis R., Krauze A.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 1

2

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Myocytes isolated from porcine coronary arteries: reduction of currents through L-type Ca-channels by verapamil-type Ca-antagonists

86%

Klockner U., Isenberg G.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 2

Myocytes were enzymatically isolated from large epicardial arteries of the pig. In the cell attached configuration, we studied currents through L-type Ca-channels. At 22°C, open channel conductance was 9 pS with 110 mM Ba²⁺ and 24 pS with 110 mM Ba²⁺ as charge carrier. According to the life time of the open state, 2 ’modes’ of gating are distinguished; mode 1 contributed time constants shorter than 1 ms, mode 2 those longer than 6 ms to the open time distribution. Mode 2 openings appeared spontaneously, more frequently with Ba²⁺ than with Ca²⁺ as charge carrier. The Ca-agonist Bay K 8644 (0.5 pM) facilitated the appearance of mode 2. Bath application of the phenylalkylamine D600 (1 µM) did not change the gating modes, but it reduced the channel openness by increasing the percentage of blank records. With whole cell recordings, we studied reduction of Ica by 1 µM D 600 at 3.6 mM [Ca²⁺] and 35°C. At a holding potential of -45 mV, D600 induced an ’initial block’ of 35% (10% at -65 mV). Upon repetitive 1 Hz pulsing (170 ms to 0 mV) an additional, ’use-dependent’ block developed with time. More negative holding potentials attenuated reduction of Ica by D600, hyperpolarizations to -100 mV had an ’unblocking’ effect. In regard to reduction of Ica, we compared the partially uncharged D 600 (membrane permeable) with the completely charged compound D890 (membrane impermeable). When applied with the bath, 1 or 10 pM D 600 reduced Ica dose-dependently whereas D 890 was ineffective. When D890 was applied via the patch electrode to the cytosol, it reduced Ica. We discuss that D 600 enters the cell in the uncharged lipid soluble form and reaches form the inside its receptor associated with the Ca-channel.

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Effects of calcium channel antagonists on the reinforcing properties of morphine, ethanol and cocaine as measured by place conditioning

58%

Biala G., Langwinski R.

Journal of Physiology and Pharmacology

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1996

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tom 47

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nr 3

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Prostaglandins and arterial wall lipid metabolism - in vitro, ex-vivo and in vivo radioisotopic studies

58%

Sinzinger H., Rogatti W.

Journal of Physiology and Pharmacology

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1994

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tom 45

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nr 1

Ograniczanie wyników

4 Journal of Physiology and Pharmacology

1 Barsys V.

1 Benetis R.

1 Biala G.

1 Garaliene V.

1 Giedraitis S.

1 Isenberg G.

1 Klockner U.

1 Krauze A.

1 Langwinski R.

1 Rogatti W.

1 Sinzinger H.

1 2014

1 1996

1 1994

1 1991

The role of external Ca2plus in the action of Ca2plus-channel agonists and antagonists on isolated human thoracic arteries

Myocytes isolated from porcine coronary arteries: reduction of currents through L-type Ca-channels by verapamil-type Ca-antagonists

Effects of calcium channel antagonists on the reinforcing properties of morphine, ethanol and cocaine as measured by place conditioning

Prostaglandins and arterial wall lipid metabolism - in vitro, ex-vivo and in vivo radioisotopic studies