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Flowery cushion gall of cacao is a disease complex with six types. Fusarium decemcellulare have been isolated from both flowery and green point galls and recognized as the etiological agent of the disease. In the present work we: i) identified by ITS-rDNA sequencing and/or taxonomy the cultivable fungal species or Operative Taxonomic Units (OTUs) associated with the five symptoms of cushion galls in cacao from Venezuela, and ii) determined the gall inducing capacity on cacao peeled seeds after 45 days of inoculation with suspensions of mycelia/ spores from distinct isolate types. The whole isolate collection rendered an abundance of 113 isolates with a richness of 39 OTUs (27) and eight identified at the species or genera levels, respectively, and in unidentified fungi. The dominant recovered species (≈36%) were F. decemcellulare and Lasiodiplodia theobromae. Some isolates of F. decemcellulare, L. theobromae, F. equiseti, Fusarium spp., F. solani, F. incarnatum, Rhizocthonia solani and Penicillium sp. were pathogenic. Some other isolates of the first six mentioned taxa behave as non-pathogenic. Furthermore, pathogenic and non-pathogenic isolates can also co-occur within a single plant and gall type. Moreover, 2–5 species within a single gall symptom in a single tree were identified (not necessarily at the same point in the tree), indicating a broad diversity of co-occurring taxa.
Effects of application of poultry manure, organo-mineral fertilizer, NPK soil or foliar applied at varying rates on cacao seedlings were studied in the nursery between January-May 2011 and December to April, 2012. Poultry manure at 40, 50 and 60g/plant significantly influenced cacao seedlings height and number of leaves over other treatments. The effect of organo-mineral fertilizer application at 50 and 60g per plant on the number of leaves was also significantly better than NPK soil or foliar applied, starting from 12 weeks after treatment application. The stem girth development under poultry manure at 50 and 60g per plant application had the best performance followed by poultry manure at 40g per plant. There were no significant differences in almost all the treatments at 5 weeks after sowing on seedlings stem girth. NPK has the longest tap root length while poultry manure had the largest average number of lateral roots followed by organo-mineral. The results thereby indicated that the use of poultry manure at 40 to 50g per plant and organomineral fertilizer as sources of nutrients had significant effects on cacao seedling quality.
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