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The detection of avocado sunblotch viroid in avocado using a real-time reverse transcriptase polymerase chain reaction

100%

Morey-Leon G., Ortega-Ramirez E., Julca-Chunga C., Santos-Chanta C., Graterol-Caldera L., Mialhe E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2018

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tom 99

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nr 2

2

Overexpression of stress-related genes in Cuscuta campestris in response to host defense reactions

100%

Rezaei H., Alamisaeed K., Moslemkhani C.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2017

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tom 98

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nr 2

3

Anti-HIV activities and mechanisms of antisense oligonucleotides

100%

Hatta T., Inagawa T., Kuwasaki T., Kinzuka Y., Takai K., Yokoyama S., Nakashima H., Yamamoto N., Takaku H.

Biotechnologia

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1996

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nr 4

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Transcriptome signature of the lactation process, identified by meta-analysis of microarray and RNA-Seq data

84%

Farhadian M., Rafat S. A., Hasanpur K., Ebrahimie E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2018

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tom 99

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nr 2

5

Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China

84%

Wei F., Wang C., Wang Z., Kong Y., Shi Y., Shi J., Qi D., Qi H., Liang L., Zhang R., Li C.

Folia Histochemica et Cytobiologica

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2014

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tom 52

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nr 3

6

RT-PCR and Northern blot analysis in search for a putative Paramecium beta-adrenergic receptor

84%

Platek A., Wiejak J., Wyroba E.

Acta Biochimica Polonica

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1999

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tom 46

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nr 3

RT-PCR and Northern blot analysis were performed in order to search for a putative beta-adrenergic receptor (β-AR) in Paramecium using several β2-adrenergic-specific molecular probes.Under strictly defined RT-PCR conditions DNA species of expected molecular size about 360 bp were generated with the primers corresponding to the universal mammalian β2-AR sequence tagged sites (located within the 4th and the 6th transmembrane regions of the receptor). This RT-PCR product hybridized in Southern blot analysis with the oligonucleotide probe designed to the highly conservative β2-AR region involved in G-proteins interaction and located within the amplified region. Northern hybridization was performed on Paramecium total RNA and mRNA with human β2-AR cDNA and two oligonucleotide probes: the first included Phe 290 involved in agonist binding (Strader et al., 1995) and the second was the backward RT-PCR primer. All these probes revealed the presence of about 2 kb mRNA which is consistent with the size of β2-AR transcripts found in higher eukaryotes.

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Molecular studies in osteogenesis imperfecta [OI] III. cDNA of COL1A1 and COL1A2 analysis using the BESS-T-Scan technique

67%

Sucharski P., Sanak M., Kostyk E., Pietrzyk J. J., Kruczek A.

Journal of Applied Genetics

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1998

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tom 39

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nr 4

A BESS-T-Scan analysis of cDNA COL1A1 and COL1A2 obtained by RT-PCR derived from five patients with sporadic forms of ostegenesis imperfecta was performed. The study was done in four patients with type I and one patient with type III OI. The analysis revealed the presence of structural changes in two regions of cDNA COL1A1 in two patients. No quantitative changes referring to COL1A2 gene were noted in any patient. The above analysis was the first application of the BESS-T-Scan technique in a molecular diagnosis of OI. The applied method seems to be useful and fulfil the basic criteria of the screening method to detect and locate mutations.

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Cytokine mRNA expression and IFN-gamma production of immunised nematode resistant and susceptible lambs against natural poly-generic challenge

67%

Pernthaner A., Cabaj W., Stankiewicz M., Davies J., Maass D.

Acta Parasitologica

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1997

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tom 42

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nr 3

IL-2, IL-4 and IFN-γ mRNA expression, and production of IFN-γ was examined in mesenteric lymph node cells (MLNC) and CD4⁺ enriched T cell populations of nematode resistant (R) and susceptible (S) line lambs by use of RT-PCR and ELISA. Five R and S line lambs that were immunised by repeated oxfendazole-abbreviated infections and 5 non-immunised R and S line lambs were used. All lambs grazed nematode infected pasture for 107 days. Immunisation enhanced the resistant status in both R and S lambs. MLNC obtained from slaughtered animals were stimulated with Con A or T. colubriformis specific antigen. Non-stimulated MLNC of immunised lambs expressed higher levels of IL-4 mRNA and lower levels of IL-2 mRNA than non-immunised lambs. MLNC of immunised R and S line lambs stimulated with antigen for 24 h expressed detectable amounts of IL-4 mRNA that was not seen in non-immunised controls. CD4⁺ T cell enriched cell populations of immunised R and S lambs and non-immunised R lambs expressed moderate to high levels of IL-4 mRNA. Con A stimulated MLNC of immunised R and S lambs expressed high levels IFN-γ mRNA and produced high amounts of IFN-γ. Lower levels were present in non-immunised controls. The results indicate that R line lambs and immunised S line lambs respond to natural nematode challenge with a predominating IL-4 cytokine response when compared to non-immunised S lambs.

Ograniczanie wyników

The detection of avocado sunblotch viroid in avocado using a real-time reverse transcriptase polymerase chain reaction

Overexpression of stress-related genes in Cuscuta campestris in response to host defense reactions

Anti-HIV activities and mechanisms of antisense oligonucleotides

Transcriptome signature of the lactation process, identified by meta-analysis of microarray and RNA-Seq data

Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China

RT-PCR and Northern blot analysis in search for a putative Paramecium beta-adrenergic receptor

Molecular studies in osteogenesis imperfecta [OI] III. cDNA of COL1A1 and COL1A2 analysis using the BESS-T-Scan technique

Cytokine mRNA expression and IFN-gamma production of immunised nematode resistant and susceptible lambs against natural poly-generic challenge