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The aim of this study was to analyse the association between factors characterising dairy herd management and the levels of somatic cell count (SCC), and total microorganisms count (TMC) in bulk tank milk. The investigations were carried out in 2005 in 187 family farms (herd size from 6 to 60 cows), situated in adjacent parts of Warmia & Mazury, and Mazowsze regions. Based on annual geometric means, all the farms were divided into those with low and elevated SCC level (<200,000 vs. 200,000–400,000 cells/mL), and with low and elevated TMC level in milk (<50,000 vs. 50,000–100,000 cfu/mL). The milk was characterised by low SCC level in 55 farms (29.4%), whereas TMC level was low in 65 farms (34.8%). The following factors were associated with the low level of SCC: small herd size, tie-stall system of cow housing, grazing cows, use of advisory service, use of antibiotics at cow dry-off, dry storing of milking clusters between milkings, post-milking teat dipping, regular use of California Mastitis Test, treatment of clinical cases of mastitis with antibiotics in selected cows only, use of whole straw in cow feeding, and use of salt-licks containing microminerals. Factors associated with the low level of TMC were: large herd size, colostrum feeding to the newborn calf by bucket, individual housing of replacement heifers older than 3 months, pipe-line system of milking, dry storing of milking clusters between milkings, pre-milking udder and teat cleaning with wet towel soaked with a disinfectant, tank system of milk cooling and storage after milking, and regular calculation of feeding rations for cows.
The occurrence of multidrug-resistant Staphylococcus aureus is an important causative agent of mastitis in cattle and of foodborne diseases. It is a worldwide concern, making it essential to develop alternative treatments to fight against the bacteria. Thus, the aim of this study is to determine the ability of carvacrol to inhibit the growth of S. aureus isolated from bulk tank milk in Turkey’s Burdur Province. All strains (n = 31) were used to investigate the antimicrobial activity of carvacrol, including the methicillin-resistant S. aureus and strains from the American Type Culture Collection and England’s National Collection of Type Cultures. The minimum inhibitory concentration (MIC) values were determined via a microdilution method, and the antimicrobial susceptibility profiles via a disk diffusion method. Antibiotic resistance was detected in 20 strains (64.5%). Multidrug resistance was observed in 8 strains (25.8%). Carvacrol exhibited strong antimicrobial activity, with MIC value at 0.058-0.234 mg/ml, in the microdilution method. Inhibition zones of carvacrol were in the range of 19 to 45 mm. The results of this study emphasize the promising role of carvacrol among new antibacterial agents that can combat S. aureus strains.
This work presents serological evidence of cattle ostertagiosis in the Lower Silesia Region (Poland), based on the measurement of antibodies in bulk tank milk (BTM) samples. It represents the first evidence of this parasite examined with the use of the ELISA test and milk samples in Poland. The prevalence of Ostertagia ostertagii antibodies was determined in BTM from 32 dairy cattle herds. Antibodies to O. ostertagii were demonstrated in all herds. The optical density ratio (ODR) varied from -0.088 to 1.024. The mean ODR value in the examined region was 0.53.
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