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An experiment was performed in a roe deer Capreolus capreolus (Linnaeus, 1758) population to assess the effect of a large-scale removal of adult males on the intensity of marking behaviour. Rubs and scrapes were censused in two 150-ha areas, one experi­mental and one control, in southeastern Sweden in May 1991 and 1992. In the experimental area, 14 males (50% of total number, 11 > 3 yrs) were culled during mid-August 1991. Removed males were replaced by the following season. Despite this, the number of scrapes, but not the number of rubs, was significantly fewer in the experimental area in May 1992 as compared to the control. This reduction could be due to a late arrival of replacement males, which would explain why the reduction involved scrapes rather than rubs, as scraping activity starts several weeks before rubbing. To cull adult males between territorial seasons is thus not an effective way to reduce rubbing intensity, and hence damages, on young forest plantations.
Total proteins and their profile(s) in three regions of epididymis, its fluid and sperm membrane extract (SME) were analysed with an objective to determine the changes in spermatozoa during epididymal maturation in goat buck. The protein content from caput to cauda increased nonsignificantly (P>0.05) while significantly (P<0.05) in tissue homogenate and spermatozoa. SDSPAGE analysis indicated the removal of >205, 205 and 95 kDa; 40 kDa proteins only in the caput and corpus spermatozoa, respectively. The proteins with molecular weight of 10, 18, 25, 35 and 20 kDa, detected only in cauda and corpus spermatozoa, respectively, seem to be associated in the maturation process of spermatozoa during epididymal transit. SDS-Page analysis of tissue and fluid indicated that the >205, 205, 195, 200, 45, 25, 18, 15 and 12 kDa proteins are structural as well as secretory proteins because of their presence both in the epididymal tissue and fluid. The nature of 100, 97, 75 and 70 kDa was found to be purely structural because of their presence in the tissue only, whereas 90, 35, 6.5 and 3.0 kDa proteins, mainly of secretory nature, due to their detection only in the fluid. Therefore, the presence of 35, 25, 18 kDa proteins both in the epididymal fluid and cauda sperm indicate that these proteins are associated with goat buck sperm maturation and fertility. Therefore, it can be concluded that the proteins of 35, 25, and 18 kDa, associated with goat sperm maturation are similar to the mentioned molecular markers in other species and can be worked out as fertility markers for goat buck semen.
We present data on fidelity to territory, and length of tenure (multi-year) for bucks of European roe deer Capreolus capreolus (Linnaeus, 1758) based on 26 radio-collared individuals that were followed for up to 5 years. Individual bucks showed a high degree of fidelity to summer territory, with consecutive year's activity centres being less than 200 m apart on average. An average 70% of one year's territory was within the borders of the previous year's territory. No buck occupied a territory which did not overlap with the previous year's territory. Activity centres of consecutive winter home ranges were on average 502 m apart, although this difference was not sig­nificant. Several cases of switching between non-overlapping winter ranges between years were observed. Annual survival was high (97%) and we observed only a single case of an old buck losing dominance on his former territory after a very hard winter. All other surviving bucks regained their dominance on their territories. It is suggested that the roe deer bucks were demonstrating an "always stay" strategy in order to gain the benefits of site familiarity. This is in keeping with the concept of roe deer territoriality being a relatively "low-risk low-gain" strategy where emphasis is placed on survival and multi-year tenure of a territory.
In a forest population of roe deer Capreotus capreolus (Linnaeus, 1758) studied in eastern Poland during the rut, does spent a greater proportion of time feeding and selecting food than bucks, during the dawn and dusk periods of grazing on meadows at the forest boundary. Bucks spent a greater proportion of time in movement {walking and running) and in orientation. These differences occurred at 3 of 4 study sites, and were unaffected by occurrence of social interactions. Although these findings contradict previous data they are predictable on the basis of optimal resource utilization, female reproductive success being determined by access to food, male reproductive success by access to mates.
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