Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 135

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 7 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  bruceloza
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 7 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
In 1987—1991 from 43 451 to 28 445 persons being often in contact with diseased animals were examined annually. The total number of registered cases of brucellosis was 423, and in the consecutive years 98, 98, 72 and 59 cases were diagnosed, respectively. Comparing the results of the previous seven years with the last five the number of infection decreased by 637. Veterinary surgeons, veterinary technicians and veterinary nurses were most often exposed to infection with brucellae.
Since 1980 Poland has been free of brucellosis. In 1981—1992 from 4.5 (35% — 1981) to 1.5 (17% — 1992) millions of cattle were examined yearly against brucellosis. In 1981 in 401 and in 1992 in 11 animals brucella infections were suspected. Serologically positive animals were culled out and slaughtered. In 1987—1991 1 840 283 (from 34 to 80% animals) in the Gorzów, Legnica, Szczecin, Wrocław and Zielona Góra voivodships were serologically examined against brucellosis. Forty six suspected animals were slaughtered and brucellosis was bacteriologically confirmed in two only cases. Bacteriological examinations of aborted fetuses and placenta from 1309 heifers or cows from these voivodships were brucellosis negative.
In this article the pharmacokinetic quality of antibacterial agents in fish has been reviewed. Oxytetracy- cline, and quinolones have been two of the most frequently used antibacterial substances in treatment of fish diseases. The use of another group of drugs is limited because of their efficacy, physico-chemical properties and human safety. In contrast to mammalian and other species, the environmental impact affects the pharmacokinetic parameters of anti-infective agents in fish. The influence of water temperature and salinity on the correlation between medications and ectothermic organisms have been emphasised. Generally, lower bioavailabilities and longer elimination times in fish have been established in contrast to other food-producing animals.
The article presents the results of serological surveys of brucellosis conducted in 1998 on cattle, pigs, sheep, goats, hares, wild boars and dogs. No positive sero-reagents among the pig population were ascertained. The rate of positive results in cattle was established as 0.00098. When B. abortus antigen was used, all serum samples from sheep and goats reacted negatively. When B. ovis antigen was used, 0.91% of sheep sera were positive. The presence of anti-Brucella antibodies was demonstrated in hares, wild boars and dogs.
The purpose was to compare the results obtained by ELISA and the following conventional methods used for the diagnosis of bovine brucellosis: Rose Bengal test (RBT), serum agglutination test (SAT) and complement fixation test (CFT). Three different ELISA test kits from various producers were used. The same 362 sera from cattle were examinated by each kit. The sera were represented by: 35 sera positive in RBT, 19 sera coming from naturally infected cattle (received from Great Britain), 3 sera recognized as positive or doubtful in national routine examination and 305 sera negative in RBT. In ELISA, depending on the kit used: 18, 19 and 26 positive and respectively 0.2 and 5 doubtful results were obtained. All remaining sera were negative. A high degree of correlation between ELISA and CFT was obtained. This indicates a high value ELISA diagnosis of bovine brucellosis.
In view of the large scale of surveys, the diagnosis of brucellosis is primarily based on serological examinations. Regarding cattle, RBT, SAT, CFT, Coombs, 2-ME and ELISA are used in Poland. The process is laborious and time-consuming. The problem of differentiation between positive reactions caused by Brucella and antigenically cross-reactive bacteria remains unresolved. The aim of the study was an application of a fluorescence polarization assay (FPA) for the examinations of sera from cattle for brucellosis. Four hundred fifty sera from cattle, including 300 sera from healthy animals, 27 sera from infected animals and 123 sera originated from confirmatory investigations, were used. The results obtained in FPA were compared to the results of RBT, SAT, CFT and ELISA. All sera from healthy animals were negative in FPA, whereas sera from infected animals were positive. Among sera from confirmatory investigations, 8 sera were positive in FPA. This likewise concerned sera positive both in CFT and ELISA. All sera positive only in RBT and SAT were negative in FPA. The results of the examinations show that FPA is a useful method for the diagnosis of brucellosis in cattle.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 7 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.