Molasses brew was applied in the production of pullulan by Aureobasidium pullulans A.p.-3. The brew was diluted to 40 g/L dry weight and supplemented with saccharose to 50,60, and 70 g/L. In the second phase of the experiment the brew was diluted to 10,20 and 30 g/L d. wt. and the media supplemented with saccharose and (NH4)2SO4. After 96 h of incubation 1.36 g of polysaccharide per 100 mL of brew media containing 20 g/L d.wt. was obtained. In the remaining samples, smaller quantities of polysaccharide were acquired. It was thus ascertained that excessive development of fungi biomass and weaker production of pullulan was observed compared with results on synthetic medium.
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