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The initial results of experiments of in vitro propagation of gooseberry (Ribes grossularia L.)

100%

Kucharska D., Orlikowska T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2015

tom 96

nr 1

Development of loop-mediated isothermal amplification [LAMP]-based SNP markers for shelf-life in melon [Cucumis melo L.]

84%

Fukuta S., Mizukami Y., Ishida A., Kanbe M.

Journal of Applied Genetics

2006

tom 47

nr 4

In this study, LAMP markers linked to shelf-life in melon (Cucumis melo L.) were developed by converting a cleaved amplified polymorphic sequences (CAPS) marker (C2). The CAPS-PCR fragments from the long-shelf-life melon (0-3) and short-shelf-life melon (Nat-2) were cloned and sequenced to construct LAMP primers. A single nucleotide polymorphism (SNP) was identified between 0-3 and Nat-2. LAMP primers were designed to detect the SNP. In the LAMP reaction to detect long-shelf-life melon, the turbidity of the templates using 0-3, F1, homozygous long-shelf-life F2 lines and heterozygous long-shelf-life F2 lines started to increase after 40 min. In contrast, the turbidity of Nat-2 and homozygous short-shelf-life F2 lines did not increase even after 90 min. In the LAMP reaction to detect short-shelf-life melon, the turbidity of the templates using Nat-2, F1, homozygous short-shelf-life F2 lines and heterozygous long-shelf-life F2 lines started to increase after 40 min. But the turbidity of 0-3 and homozygous long-shelf-life F2 lines did not increase after 90 min. This attests to the high reliability and usefulness of LAMP for marker-assisted selection.

Soil detoxication by the means of activated carbon in breeding process

67%

Spiridonov J. J., Mukhin V. M., Karpachev V. V., Gorskov V. I., Gorshkova E. K., Voropaeva N. L., Shachnev N. V., Figovsky O. L.

International Letters of Natural Sciences

2017

tom 62

The paper describes results of vegetative tests of activated carbon as a detoxiсant of herbicide remains in soil on eight varieties of summer rapeseed. The tests were performed with the purpose to align soil fertility on different breeding and test sites. The research is based on attempts to apply coal absorbent as a means of soil detoxication to neutralize herbicides remains in the process of oilseed brassica crops breeding. Several summer rapeseed varieties were used as research objects. Similar approaches have not encountered in literature references available.

Ograniczanie wyników

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 International Letters of Natural Sciences

1 Journal of Applied Genetics

1 Figovsky O. L.

1 Fukuta S.

1 Gorshkova E. K.

1 Gorskov V. I.

1 Ishida A.

1 Kanbe M.

1 Karpachev V. V.

1 Kucharska D.

1 Mizukami Y.

1 Mukhin V. M.

1 Orlikowska T.

1 Shachnev N. V.

1 Spiridonov J. J.

1 Voropaeva N. L.

1 2017

1 2015

1 2006

Wyniki wyszukiwania

The initial results of experiments of in vitro propagation of gooseberry (Ribes grossularia L.)

Development of loop-mediated isothermal amplification [LAMP]-based SNP markers for shelf-life in melon [Cucumis melo L.]

Soil detoxication by the means of activated carbon in breeding process