Wyniki wyszukiwania

1

Paraffin oil particles as microscopic probes for oxygen measurements in biological systems: ESR spin-label oximetry

100%

Ligeza A., Wisniewska A., Subczynski W. K.

Current Topics in Biophysics

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1992

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tom 16

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nr 2

2

Axenic cultivation and comparative phospholipase A2 activity of Giardia duodenalis in a serum-free medium

84%

Mata-Cardenas B. D., Hernandez-Garcia M. E., Gonzalez-Salazar F., Garza-Gonzalez J. N., Palacios-Corona R., Cortes-Gutierrez E. I., Vargas-Villarrewal J.

Acta Parasitologica

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2012

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tom 57

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nr 3

Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle’s amino acid solution, Diamond’s vitamin-tween 80 mixtures and LCR (a lipid-cholesterol — rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A2 (PLA A2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum.

3

Isolation and purification of polyclonal IgG antibodies from bovine serum by high performance liquid chromatography

84%

Stec J., Bicka L., Kuzmak J.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 3

4

Mechanism of antigenic variation in Shigella flexneri bacilli. III. Complement sensitivity of S.flexneri 1b strains and their 3b variants selected either as Lac plus recombinants or phage F2 resistant mutant

84%

Doroszkiewicz W., Kopocinski B., Lachowicz T. M.

Archivum Immunologiae et Therapiae Experimentalis

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1995

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tom 43

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nr 1

5

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Quantitative evaluation of biofilm formation in yeast nitrogen base (YNB) broth and in bovine serum (BS) of Candida albicans strains isolated from mucosal infections

67%

Krzysciak P.

Wiadomości Parazytologiczne

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2011

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tom 57

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nr 2

Candida albicans is a major human fungal pathogen especially as an etiologic agent of opportunistic oral and genital infections. Moreover, C. albicans can be involved in the deep infections and recent evidence suggests that the majority of diseases produced by this pathogen are associated with biofilm growth. The aims of this study were to evaluate biofilm production ability of C. albicans strains isolated from different sources, and to evaluate the effect of serum for enhancement the growth of biofilm. The strains used in this study were obtained from three sources; 12 from feces of patients with gastrointestinal disturbances, 13 from the oral cavity of patients with oral candidiasis, and 16 from the vagina of patients with Candida vulvovaginitis (CVV). Polystyrene 96-well plates were used to grow biofilms and crystal violet (CV) staining method was used to evaluate the growth. There were no differences in biofilm growth expressed as CV absorbance between C. albicans strains from different origins neither in Yeast Nitrogen Base broth (YNB) or in bovine serum (BS) (ANOVA, P=0.1648, P=0.5106, respectively). In the BS, the biofilm production was greater than in YNB medium for all samples (ANOVA, P=0.0003).

6

Mechanism of antigenic variation in Shigella flexneri bacilli. IV. Role of lipopolysaccharides and their components in the sensitivity of Shigella flexneri 1b and its Lac plus recombinant to killing action of serum

67%

Doroszkiewicz W.

Archivum Immunologiae et Therapiae Experimentalis

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1997

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tom 45

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nr 2-3

7

The effect of commercially available media on the structure of ovaries of the house cricket cultured in vitro

67%

Marewicz E., Klag J.

Folia Biologica

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1994

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tom 42

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nr 3-4