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Influence of isoflavones on secretion of oestradiol-17beta by bovine granulosa cells in vitro

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Ras M., Kaminski T., Janowski T., Zdunczyk S.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 3

Granulosa cells were isolated from large (≥8 mm) follicles and incubated for 24 h without or with LH (100 ng/mL), daidzein, genistein or equol in doses of 0.5, 5, 10, and 50 µg/mL each or combination (3:13:39) of these isoflavones at a dose 50 µg/ml. The concentration of oestradiol-17ß in incubation media was measured by RIA. All the isoflavones at used doses decreased significantly basal and LH-stimulated secretion of oestradiol-17ß by granulosa cells in a dose dependent manner. The combination of these isoflavones also inhibited significantly oestradiol-17ß secretion by the cells. These data suggested that decreased secretion of oestradiol-17ß caused by dietary isoflavones could be a reason of silent heat in cows.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Janowski T.

1 Kaminski T.

1 Ras M.

1 Zdunczyk S.

1 2010

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Influence of isoflavones on secretion of oestradiol-17beta by bovine granulosa cells in vitro