This report describes the treatment of osetomyelitis of the femur of 9-month-old dog with the use of heterologous carbonated nanocrystal bone mineral and collagen, stabilised by a bone plate. In this dog, due to mistakes during earlier stabilisation, bone infection and sequestration had arisen. After antibiotic therapy, plating stabilisation was carried out. Osteosynthesis with the use of dynamic compression plate and heterologous carbonated nanocrystal bone mineral and collagen was an effective method of stabilisation, which led to the bone healing. Clinical significance: Heterologous carbonated nanocrystal bone mineral and collagen in preparation OsteoBiol turned out to be an effective material in the treatment of the lack of bone. The clinical case outlined is the first notification, which describes the applying of the preparation in therapy of osetomyelitis of the femur in a dog.