Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 14

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  blood glucose
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
5
Content available remote

Metformin-induced regulation of the intestinal D-glucose transporters

80%
Metformin is an orally administered drug that lowers blood glucose and improves insulin sensitivity in patients with non insulin-dependent diabetes. Although the antihyperglycemic effect of metformin has been extensively studied, its cellular mechanism(s) of action (including the effect on enterocyte) remains to be defined. This study was designed to examine the effect of metformin on glucose transporters in enterocyte. Na+-dependent glucose transporter-1 (SGLT-1) activity was followed as glucose-induced short-circuit current (Isc) in Ussing chambers. The effect of metformin (10 µmol/L, 3 min) on transmural glucose transport was studied in isolated rat jejunal loops. Its impact on abundance of transporters SGLT-1 and GLUT2 in jejunal brush border membranes (BBM) and its effect on the phosphorylation of AMP-activated protein kinase (AMPK) 2 subunit was studied by western blot. Acute effect of metformin was also measured in vivo by oral glucose tolerance test (OGTT). Metformin markedly inhibited glucose-induced Isc (~77%) after mucosal addition. In addition, metformin reduced the glucose-induced abundance of SGLT-1 in BBM and increased those of GLUT2, concomitantly increasing the phosphorylation of intracellular AMPK2. This effect of metformin was also observed using non-metabolizable sugar 3-O-methyl glucose. Transmural glucose transport measured in vitro was increased by 22% under metformin. Finally, oral metformin markedly increased glucose tolerance in OGTT. In conclusion, metformin slightly increases intestinal glucose absorption by inducing a re-distribution of glucose transporters in BBM through AMPK control in enterocyte. In addition to its action to other splanchnic tissues, this could constitute a peripheral signal contributing to the beneficial effect of metformin on glucose tolerance.
A study was conducted to determine the distribution of subclinical pregnancy toxemia by the evaluation of serum glucose, BHB, urea, and cortisol concentrations in pregnant ewes. Eight hundred and nine blood samples were collected from 497 pregnant, 242 lambed, and 70 aborted ewes. The concentrations of BHB, glucose and urea were assessed by spectrophotometer and cortisol by ELISA. Mean urea and BHB concentrations in pregnant ewes were greater and glucose was lower than in lambed and aborted ewes. Cortisol concentrations in pregnant ewes were higher than lambed but lower than aborted ewes. Except for BHB, the mean comparison of parameters showed a significant difference (P < 0.01) in that the concentrations were similar in all groups. A negative correlation (P < 0.05) in urea concentration was observed between pregnant and lambed ewes. There were correlations (P < 0.01) between BHB and glucose in pregnant ewes, BHB and cortisol in lambed ewes and BHB and urea in aborted ewes. Regarding the references, the concentration of BHB > 0.7 mmol/l is considered as subclinical pregnancy toxemia; the distribution and percent of ewes in pregnant, lambed and aborted groups were 61 (12.3%), 17 (7%) and 6 (8.57%), respectively. Similarly, for glucose < 20 mg/dl were 76 (15.3%), 38 (15.7%), 10 (14.3%) and for cortisol > 52 ng/ml were 28 (8.54%), 1 (0.6), 5 (10.2%), respectively. Significant differences were found for the BHB and cortisol groups. The highest subclinical pregnancy toxemia occurred among pregnant and aborted ewes. Mean comparison of parameters in the group with BHB > 0.7 mmol/l showed a significant difference (P < 0.01) for urea and cortisol concentrations and in the group of glucose < 20 mg/dl and cortisol > 52 ng/ml showed differences only for the BHB concentration. Thus it is concluded that hypoglycemia, uremia and high cortisol concentration in late pregnancy could be considered as subclinical pregnancy toxemia up to 12% that should be seriously considered in order to support prevention or enact treatment.
Relations between the concentration of glucose and urea in blood plasma and the qualitative ejaculate parameters were investigated in 24 breeding bulls (13 Holstein and 11 Czech Spotted) over a 12-month period. The average concentrations of plasma glucose and urea were 3.32 mmol ∙ 1⁻¹ and 3.57 mmol ∙ 1⁻¹ , respectively. No statistically significant differences in the concentrations of glucose and urea (i.e. 3.34 vs. 3.30 mmol ∙ 1⁻¹ and 3.53 vs. 3.61 mmol ∙ 1⁻¹, respectively) were observed between the Holstein bulls and Czech Spotted bulls. A statistically significant (P ≤ 0.05) positive correlation was found between the concentration of plasma urea and the ejaculate volume for the whole investigation period (rp = 0.19). Similarly, the correlation between the concentration of plasma urea and sperm motility was positive (rp = 0.11), whereas the increase in plasma urea concentration negatively influenced sperm concentration ( rp = -0.10). Correlations between plasma glucose concentrations and the respective parameters of ejaculate quality were similar in magnitude, i.e. ejaculate volume ( rp = 0.12), sperm motility ( rp = 0.12) and sperm concentration ( rp = -0.08). During the investigations the coefficients of correlation between plasma glucose and urea concentrations as well as qualitative parameters of the ejaculate were found to be negative in months when concentrations of glucose and urea in the blood plasma of the bulls markedly changed.
The changes in some blood biochemical parameters were studied in the freshwater fish N. notopterus under saline exposure for a longer period of 30 days. The blood biochemical parameters are glucose, protein, triglycerides and cholesterol. All these parameters exhibited increased level except of blood glucose which remained unchanged as observed after the termination of saline exposure. Thus it indicates that although fish survives and able to tolerate extreme saline condition as there was no mortality during the exposure period. The increase of blood biochemical parameters can be considered as a kind of saline stress response particularly on lipid derivatives.
The aim of the study was to assess the effect of nutritional status of the organism on the sensory specific satiety (SSS) in young adults and to determine the relationship of blood glucose concentration and SSS. The study was carried out on a set of 18 individuals (6 persons in each group with BMI values: <20, 20-25 and >25 kg/m2, respectively), which rated the pleasantness of foodstuffs with different sensory properties (milk chocolate, crackers, grapefruit and apples) before and after ad libitum consumption of milk chocolate. The SSS was observed in all the tested individuals, irrespective of their BMI value. No statistically significant correlation was found between the change in blood glucose concentration 2 minutes after the consumption of chocolate and the occurrence of SSS. A dependence between these parameters was found 60 min after chocolate consumption.
The biochemical characteristics of tench, Tinea tinea (L.), stocking material were examined with regard to the following parameters prior to and after wintering: bactericidal activity of the blood serum; blood glucose level (BG); total proteins (TP); blood serum protein fractions (albumins, and α-, β-, and γ-globulins). The study was conducted over the course of three years (1996 - 1998) on clinically healthy two-summer-old (fall) and two-year-old (spring) tench that ranged in body weight from 30 to 80 g. The study indicated that one of the parameters that characterized the resistance of tench in the wintering period was the relative stability of the bactericidal activity of the blood serum. Seasonal dynamics in the levels of blood glucose and total serum proteins were determined, and the levels of the former were higher in fall and lower in spring. The specific metabolic nature of different protein fractions in the blood serum of tench was characterized by percentage decreases of albumins and α- and γ-globulins and by increases of β-globulins.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.