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1

Analysis of in vivo- and in vitro-derived pig expanded blastocysts based on DNA fragmentation

100%
Bryla M., Trzcinska M., Wieczorek J.
Animal Science Papers and Reports
|
2009
|
tom 27
|
nr 1
59-68
The aim of the study was to investigate the competence of in vivo- and in vitro-derived pig expanded blastocysts by analysing DNA fragmentation using TUNEL. A total of 533 porcine expanded blastocysts were examined, and results were evaluated using Fisher’s test. Significant differences in the incidence of fragmented nuclei (detected by the TUNEL reaction) and all nuclei (detected by DAPI) were identified between in vivo- and in vitro-derived embryos at the expanded blastocyst stage. The total numbers of nuclei observed in in vivo-derived embryos were significantly different from those in in vitro-cultured embryos (89.1±13.4 and 47.7±25.1, respectively). TUNEL index in In vitro-cultured embryos (28.3%) was significantly higher (P<0.01) than in in vivo-derived blastocysts (4%). These findings indicate that in vivo- and in vitro-derived expanded blastocysts consisting of a small number of cells are characterized by a high incidence of DNA fragmentation. The total number of nuclei in in vivo- and in vitro-derived blastocysts correlated negatively with the number of TUNEL-positive nuclei (r = -0.51; P<0.0001) and the TUNEL index (r = -0.69; P<0.0001), whereas the number of TUNEL-positive nuclei was positively correlated with the TUNEL index (r = 0.95;P<0.0001). Moreover, significant differences were observed between embryos collected from individual experiments.
2

Stereological studies of mitochondria during the implantation of mouse blastocytes

100%
Cieciura L., Jaszczuk-Jarosz B., Pietrzykowska K.
Folia Histochemica et Cytobiologica
|
1986
|
tom 24
|
nr 3
3

BCB test utility evaluated by caspase-3 activity in blastocysts developed from bovine oocytes with different G6PDH level

100%
Opiela J., Rynska B., Katska-Ksiazkiewicz L.
Annals of Animal Science
|
2010
|
tom 10
|
nr 1
27-38
4

Evaluation of mouse preimplantation embryos cultured in media enriched with insulin-like growth factors I and II, epidermal growth factor and tumour necrosis factor alpha

100%
Kurzawa R., Glabowski W., Wenda-Rozewicka L.
Folia Histochemica et Cytobiologica
|
2001
|
tom 39
|
nr 3
5

In vitro survival and hatching of mink embryos in diapause

100%
Moreau G. M., Smith L. C., Song J., Murphy B. D.
Zeszyty Naukowe. Przegląd Hodowlany
|
1996
|
tom 27
6

Activity of delta5-3beta-hydroxysteroid dehydrogenese and steroid hormones content in early preimplantation horse embryos

84%
Paulo E., Tischner M.
Folia Histochemica et Cytobiologica
|
1985
|
tom 23
|
nr 1-2
7

Some factors influencing the efficiency of demi-embryos production from zona-drilled bovine IVP blastocysts

84%
Katska L., Shioya Y., Takenouchi N., Skrzyszowska M.
Roczniki Naukowe Zootechniki
|
1999
|
tom 26
|
nr 4
8

Influence of medium on viability of bovine embryos produced in vitro after their biopsy

84%
Lasiene K., Valanciute A., Lasys V.
Medycyna Weterynaryjna
|
2005
|
tom 61
|
nr 12
9

Lipid content in pig blastocysts cultured in presence or absence of protein and vitamin E or phenazine ethosulfate

67%
Romek M., Gajda B., Krzysztofowicz E., Kepczynski M., Smorag Z.
Folia Biologica
|
2011
|
tom 59
|
nr 1-2
10

Genetical and biotechnological methods of utilization of female reproductive potential in mammals

67%
Katska-Ksiazkiewicz L., Lechniak-Cieslak D., Korwin-Kossakowska A., Alm H., Rynska B., Warzych E., Sosnowski J., Sender G.
Reproductive Biology
|
2006
|
tom 06
|
nr Suppl.1
11
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Morphology and developmental potential of bovine parthenotes after spontaneous activation in vitro

67%
Lechniak D., Cieslak D., Sosnowski J.
Journal of Applied Genetics
|
1998
|
tom 39
|
nr 2
Spontaneous parthenogenetic activation of bovine oocytes in an in vitro maturation and fertilization system (IVM/IVF) is described. Altogether, 1403 follicular oocytes, collected by the aspiration method, were matured in vitro and then cultured without insemination in the same conditions as a group of inseminated oocytes. After 48-72 h of additional culture, 141 oocytes (10%) were found to be spontaneously activated. Morphological evaluation revealed that the number of blastomeres within parthenotes ranged from 2 to 16 cells, with a minority (15.7%) comprising of 9-16 blastomeres. According to a cytogenetic analysis, only 1.2% of the analysed parthenotes consisted of more than 9 cells. Parthenotes may not be distinguished from embryos produced in vitro and spontaneous parthenogenetic activation in an IVM/IVF system indicates suboptimal culture conditions. A group of non-inseminated oocytes should be included in each experiment to serve as a control. Spontaneously activated bovine parthenotes only occasionaly developed beyond the 8-blastomere stage in a common IVM/IVF system. The incidence of parthenotes interferes with the efficiency of in vitro embryo production but it is doubtful whether it lowers the pregnancy rate after transfer of IVF embryos.
12

In vitro assessment of the influence of different leukocyte count lavaged from normal and inflamed cow uterus on the mouse embryo development

67%
Klucinski W., Sitarska E., Dembele K., Winnicka A., Sikora J., Kleczkowski M.
Archivum Veterinarium Polonicum
|
1995
|
tom 35
|
nr 1-2
13

Influence of DNA concentration on the efficiency of bovine blastocyst production after WAP-bGH microinjection into germinal vesicle of immature oocytes, zygotes and embryos obtained in vivo or in vitro

67%
Jura J., Smorag Z., Katska L., Skrzyszowska M., Houdebine L. M., Rynska B.
Biotechnologia
|
1997
|
nr 4
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