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  1. 17 Bulletin of the Veterinary Institute in Puławy

  2. 16 Polish Journal of Veterinary Sciences

  3. 8 Medycyna Weterynaryjna

  4. 3 Weterynaria w Praktyce

  5. 1 Acta Parasitologica

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  1. 7 Gajecka M.

  2. 7 Gajecki M.

  3. 7 Jakimiuk E.

  4. 6 Janowski T.

  5. 6 Madej J. A.

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  1. 3 2020

  2. 3 2018

  3. 1 2017

  4. 2 2014

  5. 2 2012

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1

Clinical evaluation of the complications founf in bitches after total bilateral mastectomy operations

100%
Kirsan I., Ucmak M., Apaydin S.
Medycyna Weterynaryjna
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2005
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tom 61
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nr 05
2

Efficiency of vaginal electrical impedance to determine the stage of the reproductive cycle in bitches

88%
Gurler H., Koldas E., Binli Onyay F., Akcay A.
Medycyna Weterynaryjna
|
2018
|
tom 74
|
nr 03
A study was carried out to compare the efficiency of vaginal electrical impedance with serum progesterone profile, cytological examination and clinical findings. Vaginal electrical impedance values varied at the different stages of the estrous cycle, with the highest value during the transition into estrous and the lowest in diestrus (P < 0.05). There was a negative statistical correlation between serum progesterone values and the impedance values (36%) (P < 0.001). Vaginal electrical impedance was a faster and cheaper method than progesterone assessment. It was more reliable than vaginal cytology and clinical evaluation. In conclusion, a combination of vaginal electrical impedance measurements and progesterone evaluation was a more useful method for determination of the optimal breeding time in bitches.
3
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Content available

The in vitro culture supplements and selected aspects of canine oocytes maturation

75%
Bukowska D., Kempisty B., Piotrowska H., Zawierucha P., Brussow K. P., Jaskowski J. M., Nowicki M.
Polish Journal of Veterinary Sciences
|
2012
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tom 15
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nr 1
The maturation of oocytes is one of the most important steps determining their developmental competence. Due to the low percentage of oocytes of bitches that reach the MII stage, searching for reagents that may stimulate the growth and maturation of oocytes is still present in this species of mammals. The most important media supplements include gonadotropins (LH, FSH, hCG), growth factors (IGF, TGF, EGF, FGF), progesterone and follicular fluid. It is suggested that the supplement of EGF, and/or follicular cells may have an important influence on the percentage of cells that reach the MII stage. Despite plenty of research based on the improvement of bitch oocytes in vitro culture, the results obtained are still unsatisfactory. Moreover, in the long stages of canine oocytes maturation many molecular and morphological modifications (including changes in mitochondria structure and configuration in the cytoplasm) are involved. In this article, the influence of selected media supplements on the efficiency of bitch oocytes in vitro maturation was described. The molecular and morphological modifications during canine oocytes maturation were also considered in the text.
4

Course of uterine involution and the level of progesterone in bitches with endometritis-pyometra complex treated with aglepristone

75%
Domoslawska A., Janowski T., Jurczak A.
Bulletin of the Veterinary Institute in Puławy
|
2010
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tom 54
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nr 1
19-23
The aim of the study was to estimate the uterus involution and progesterone level after antigestagene therapy in 12 bitches with a closed form of endometritis-pyometra complex (EPC). The bitches were of pure and mixed breeds, between 2 and 13 years of age. All the animals were treated with the same pattern: aglepristone was administrated at the dose of 0.33 mL/kg, s.c. on days 1, 2, 7, and 14. Additionally, amoxycyline was given at the dose of 1 mL/l0kg, s.c. every 48 h for 10 d. Complete elimination of puss masses and uterus involution were observed after 7-14 d of the treatment. Progesterone concentration was highly increased at the beginning of the treatment and was decreasing to low values during the first week of the therapy. Detectable uterus changes in the USG examination and physiological health status are not the only recovery criteria, but, most importantly, lack of recurrence of the EPC and the ability to deliver healthy progeny.
5

Immunohistochemical localisation of oestrogen and epidermal growth factor receptors of the bitch uterus in the sexual cycle

75%
Ozyurtlu N., Sagsoz H., Saruhan B. G., Zonturlu A. K., Ketani M. A., Akbalik M. E.
Bulletin of the Veterinary Institute in Puławy
|
2010
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tom 54
|
nr 2
193-200
The localisation of oestrogen (ER) and epidermal growth factor receptors (EGFR) in the various cell types of the bitch uterine was determined. In this study, 23 adult, healthy crossbred bitches brought to the clinic for ovariohisterectomy were used. ER and EGFR positive staining was detected in all cell types of the uterus. A distinct staining was seen in the luminal and glandular epithelium; while stromal and myometrial cells showed weak or moderate staining. The endothelial and smooth muscle cells of the vessels in the endometrium and myometrium sometimes appeared positive. No staining was observed in the mesothelium. The results of this study suggested that ER and EGFR were expressed at various levels in different cell types of bitch uterus. In light of the previous studies, and data of the presented investigations, it may be necesssary to elicit the harmonious proliferation and differentiation of epithelial and stromal cells that are considered essential for the preparation of the uterus for implantation.
6
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Etiology, symptoms and treatment of uterine torsion in domestic animals

75%
Kacprzak K. J., Jurka P., Max A., Czerniawska-Piatkowska E., Bartyzel B. J.
Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica
|
2014
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tom 32
Skręt macicy to schorzenie występujące u prawie wszystkich zwierząt domowych dość rzadko, z wyjątkiem bydła. Problem ten występuje najczęściej w drugiej połowie ciąży lub w trakcie porodu jako przyczyna niestosunku porodowego. Opisywany jest jako skręcenie się narządu prostopadłe do jego osi długiej. Nie wyjaśniono w wystarczający sposób etiologii tej choroby, wydaje się jednak, iż czynnikiem ryzyka predysponującym do skrętu jest niestabilność ciężarnej macicy. Zewnętrzne objawy kliniczne nie są specyficzne. Diagnozy problemu dokonuje się poprzez badanie przez pochwę, badanie rektalne oraz metodami obrazowania jamy brzusznej. U zwierząt gospodarskich leczenie ukierunkowane jest na pozostawienie funkcjonującej macicy, w związku z czym postępowanie chirurgiczne nie jest techniką z wyboru. Wykorzystuje się takie metody, jak ręczne odkręcanie narządu przez pochwę, użycie drążka odkręcającego oraz przetaczanie zwierzęcia przez jego oś długą. Jeżeli przedstawione sposoby okażą się nieskuteczne, wykonywany jest zabieg operacyjny. U psów i kotów postępowaniem z wyboru jest leczenie chirurgiczne. Do niedawna preferowanym postępowaniem u małych zwierząt był zabieg usunięcia macicy wraz z jajnikami, co uniemożliwiało dalszy rozród tych zwierząt. Obecnie stosuje się również nową technikę – jednostronne usunięcie jajnika i rogu macicy.
7

Effect of botulinum toxin [BTX] on the chemical coding of nerve fibres supplying the canine urinary bladder

75%
Lew S., Bossowska A., Radziszewski P., Majewski M., Kwiatkowska M., Kuleta Z.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
|
nr 1
69-74
The objective of this study was to investigate the distribution and chemical coding patterns of nerve fibres supplying the canine urinary bladder before and after botulinum toxin (BTX) injection. The experimental material comprised six bitches. The injection of the BTX into the urinary bladder wall in dogs clearly altered the bladder's innervation pattern, indicating that BTX affects the components of both the sensory and parasympathetic nervous systems, and that degenerative changes are accompanied by restorative processes.
8

Lymphocyte subpopulations in the bitches with pyometra treated with aglepristone

75%
Jurka P., Faundez R., Winnicka A.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
|
nr 4
The purpose of the study was to compare flow cytometric and haematologic variables in dogs with spontaneous endometritis pyometra complex (EPC) treated with aglepristone to healthy controls. Peripheral blood mononuclear cells: CD3, CD4, CD8, and B lymphocytes (CD21) were analysed by flow cytometry and white blood cell count. Significant differences were observed (P≤0.01) between control (C) and study (S) group in the total number of leukocytes, monocytes and granulocyte populations (without lymphocytes) on beginning, after 7 d, and return to reference value after 14 d of the treatment. The percentage of the T-cell (CD3+) at the beginning was 47.22 ±9.64% of total lymphocytes, in contrast to B lymphocytes (CD21+) that represented the smallest percentage of 14.24 ±7.74% (P≤0.01). The percentage of the lymphocytes CD4+ was 27.42 ±5.53% and CD8+ was 25.18 ±4.36%. The percentage of CD3+ lymphocytes was increasing throughout the experiment in group C and gradually decreased in group S from 14th to 28th d of dioestrus. No differences in the number of CD3+, CD4+, CD8+, and CD21+ lymphocytes between group C and S on the 14th and 28th d of dioestrus (P≥0.05) were observed. The number of CD8+ cells in group S decreased gradually from day 14 to 28 but no statistical differences were noted. Treatment of pyometra with aglepristone decreased the number of leukocytes, monocytes, and granulocytes to referential value but statistically significant influence on the level of subpopulations of T and B lymphocytes was not observed. The results enabled to estimate for the first time the number of lymphocyte subpopulations in dioestrus in both healthy bitches as well as in those suffering from pyometra.
9

Leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches

75%
Magro A. G., Assis V. P., Silva L. C., Tafuri W. L., Silva S. O., Melo M. N., Valle G. R.
Acta Parasitologica
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2017
|
tom 62
|
nr 3
10

Influence of zearalenone micotoxicosis on selected immunological, haematological and biochemical indexes of blood plasma in bitches

75%
Gajecka M., Jakimiuk E., Skorska-Wyszynska E., Zielonka L., Polak M., Paluszewski A., Rybarczyk L., Gajecki M.
Polish Journal of Veterinary Sciences
|
2004
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tom 07
|
nr 3
The immunological, haematological and biochemical analyses of blood plasma in bitches with 50 days lasting induced zearalenone micotoxicosis were carried out. It can be indirectly suggested that the inhibition of the humoral reaction of the organism, stimulation of detoxification effect in the liver and decreased cellular answer took place.
11

Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system

75%
Gajecka M., Jakimiuk E., Polak M., Otrocka-Domagala I., Janowski T., Zwierzchowski W., Obremski K., Zielonka L., Apoznanski J., Gajecki M.
Polish Journal of Veterinary Sciences
|
2004
|
tom 07
|
nr 1
Both, earlier investigations and complete formulas of pet food for dogs show that professionally prepared pet food is based in 30% on the material of the plant origin. Our own investigations prove that animal feeding stuffs of the plant origin are very often vectors of many undesirable substances (including micotoxins) that are unfavourable for carnivores. Zearalenone is a micotoxin, which frequently occurs among them. It was revealed in pet food in quantities from 5.0-299.5 μg/kg- It is a very dangerous xenobiotic because of its quick and easy absorption in the organism after per os application in monogastric animals. It is also a sterid and despite its plant origin it binds estrogen receptors in the reproductive tract and causes its hypo- and dysfunction. It is clearly visible in young, growing organisms and those during the menopause. In bitches, dysfunctions of the reproduction system, such as: endometritis-pyometra syndrome, prolonged oestrus, ovarian cysts and others are often found. It is supposed that specific for this species hormonal regulation of the reproduction processes based on the long progesterone and prolactin cycles, and on high sensibility to estrogens plays an important role in the etiopathogenesis of these dysfunctions. The application of hormones in bitches in therapeutic and biotechnical (contraception) purposes are the other factors, which should be taken into account in this aspect. There are some suggestions that therapeutic management, unfortunately connected with mistakes in the medical art, is often a cause of pathological condition in a reproductive organ in bitches. However, another reason of these disorders may be that bitches take zearalenone with the commercial pet food. The long-lasting intake of the feed with a low dose of this micotoxin may be the factor, which complicates hormonal regulation of the reproduction processes and is the cause of many disorders. Our own pilotage investigations showed that application of zearalenone at a dose of 200 μg/kg b. w. for 7 days leads to apoptotic-like changes in granule cells in the bitch reproductive system, atresia of follicular cells and oedema and hyperplasia of the oviductal and uterine cells. They could also indicate a follicular phase of the oestrus cycle in bitch. However, it did not take place.
12

Involvement of CD8plus cells in protective mechanisms in canice mammary adenocarcinomas [short communication]

75%
Nowak M., Madej J. P., Rossowska J., Madej J. A.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 3
The study aimed at demonstration of involvement of CD8+ cells in protective mechanisms in canine mammary gland carcinomas. Material for the studies involved metastases to lungs sampled at autopsy from crossbreed bitches aged 11 to 13 years. The tumours were verified histopathologically as adenocarcinomas. The presence of CD8+ cells infiltrating the examined tumour tissues was estimated in cryo-sections by immunofluorescence methods. Parental tumours free of metastases were accompanied by slight infiltration of CD8+ cells. In the case of metastasing tumours, and especially in their pulmonary metastases, the increased number of CD8+ cells was observed. Taken together, metastasing potential of canine mammary adenocarcinomas was positively correlated with the number of CD8+ cells in the tumour stroma.
13

Diffuse cystic lymphangiectasia in a bitch

75%
Oral H., Gungor O., Sozmen M., Pancarci S. M., Kacar C.
Bulletin of the Veterinary Institute in Puławy
|
2007
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tom 51
|
nr 1
An eight-year-old German shepherd crossbreed clinically healthy bitch was ovariohysterectomized on the owner's request. The ovariohysterectomy operation was performed on the left flank area, under general anaesthesia. In both uterine horns, diffuse 0.1-3 cm in length cystic structures filled with serous fluid were detected during macroscopic examination. Histopathological examination of the uterus revealed that the pathological tissue was diffuse cystic lymphangiectasia.
14

Use of a decreased dose of cabergoline to treat secondary anoestrus in bitches

75%
Cirit U., Bacinoglu S., Tas M., Alkan S.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 1
The aim of the study was to determine whether a dose (0.6 µg/kg/d) quite lower than the prolactin-lowering dose of cabergoline, prepared for humans, would be a safe and effective method for the stimulation of oestrus in bitches at secondary anoestrus or late anoestrus. Twenty-four pure blood bitches from various breeds were used in the study at their already determined periods of anoestrus. The treatment group included bitches at late and prolonged anoestrus. Eight bitches that had not shown any signs of oestrus for the preceding 370 to 485 d formed the secondary anoestrus group. Eight of the 16 bitches at late anoestrus (days 165-280) have accomplished the late anoestrus group and another 8 have been chosen randomly for the control group (untreated). Cabergoline was orally administrated until day 2 after the onset of pro-oestrus or for a maximum of 42 d. Blood samples were taken daily from each bitch during the first 5 d of behavioural oestrus to measure progesterone concentrations. In the secondary anoestrus and late anoestrus groups, oestrus was induced on days 4-14 and 12-45 at a ratio of 75.0% (6/8) and 87.5% (7/8), respectively. The mean pro-oestrus and behavioural oestrus durations, serum progesterone concentrations on day 5 of oestrus, ovulation rates, pregnancy rates, and the mean litter sizes in secondary anoestrus, late anoestrus, and control groups were found to be similar. None of the dogs had any adverse gastrointestinal effects associated with cabergoline administration. The results of the present study suggest that the administration of 0.6 µg/kg/d of cabergoline is a safe and effective treatment for secondary anoestrus in bitches.
15

First report of vaginal prolapse in an ovariohysterectomised bitch - a case report

75%
Nak D., Nak Y., Yilmazbas G.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 3
The first case of vaginal prolapse, type III, in ovariohysterectomised 3-year-old Chow-Chow breed bitch, weighing 19 kg, was described. The bitch had developed vaginal prolapse four months after the ovariohysterectomy. Circumferential excision of the prolapsed tissue was performed and finally the bitch recovered as free of the problems. There was no evidence of recurrence of the prolapse in the period of following two years.
16

Studies on histogenesis of mixed tumours on the mammary gland in bitches

63%
Smiech A., Lopuszynski W., Nozdryn-Plotnicki Z.
Polish Journal of Veterinary Sciences
|
2002
|
tom 05
|
nr 4
17

Copper content in neoplastic and healthy mammary glands in dogs

63%
Skibniewska E. M., Kosla T., Skibniewski M.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 2
269-272
The aim of the investigation was to confirm the hypothesis put forward in human research that copper is cumulated in the neoplastic tumours of the mammary gland. The research material included the post mortem collected healthy mammary glands of bitches and mammary gland neoplastic tumours obtained during routine surgical treatments. The histopathological examinations revealed that among tumours of epithelial origin, the most frequent were adenocarcinomas, which comprised 64% of all neoplastic lesions. The second group included carcinomas - 26%, whereas 10% were the tumours of mesenchymal origin. The lowest copper content was observed in healthy mammary glands. In the tissues with neoplastic lesions, the content of copper was at a much higher level. Statistical analysis revealed a significant difference (P≤0.05) in the copper content between the investigated groups. The performed analyses showed that mammary gland neoplasms cumulate copper ions, and a much higher concentration of this element is observed in the tumours of epithelial origin.
18

Transcriptomic signature of cell lines isolated from canine mammary adenocarcinoma metastases to lungs

63%
Krol M., Polanska J., Pawlowski K. M., Turowski P., Skierski J., Majewska A., Ugorski M., Morty R. E., Motyl T.
Journal of Applied Genetics
|
2010
|
tom 51
|
nr 1
37-50
Metastasis is a final step in the progression of mammary gland cancer, usually leading to death. Potentially, a molecular signature of metastasis can be defined via comparison of primary tumors with their metastases. Currently, there is no data in the literature regarding the molecular portrait of metastases in dogs and only few reports regarding human cancer. This is the first report describing the transcriptomic signature of canine cancer metastatic cells. Two adenocarcinoma cell lines isolated from the canine mammary gland (CMT-W1 and CMT-W2) were compared with cell lines isolated from their lung metastases (CMT-W1M and CMT-W2M) with regards to the following cytometric parameters: cell cycle, ploidy, Bcl-2 expression, susceptibility to induced apoptosis, and transcriptomic profile. Cytometric analyses revealed significant differences in cell cycle and antiapoptotic potential between the examined cells. Using oligonucleotide microarrays, we found 104 up-regulated genes in the metastatic cell line CMT - W1M and 21 up-regulated genes in the primary CMT -W1 cell line. We also found 83 up-regulated genes in the CMT-W2M cell line and only 21 up-regulated genes in the CMT-W2 cell line. Among the up-regulated genes in both metastatic cell lines, we found 15 common genes. These differently expressed genes are involved mainly in signal transduction, cell structure and motility, nucleic acid metabolism, developmental processing, and apoptosis (GHSR, RASSF1, ARF1GAP, WDR74, SMOC2, SFRP4, DIAPH1, FSCN1, ALX4, SNX15, PLD2, WNT7B, POU6F2, NKG7, and POLR2F). Seven of them are involved in a cellular pathway dependent on ghrelin via growth hormone secretagogue receptor (GHSR). Our results suggest that this pathway may be essential for mammary cancer cells to have a metastatic potential.
19

Dorsal perineal hernia in a bitch

63%
Galanty M., Mastalerz T., Turek B.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 08
919-921
The paper describes a case of a dorsal type of perineal hernia in an 11-year-old bitch of the Yorkshire terrier breed. The hernia was situated between the levator ani muscle and the coccygeal muscle. The hernial sac contained small intestines and a small amount of the retroperitoneal fatty tissue. Additionally, both sided inguinal hernias and collapse of trachea were diagnosed in the dog. In the discussed case the perineal hernia was operated on by the means of placing sutures on the levator ani muscle and the coccygeal muscle. No recurrence of the problem was observed during 19 months following the surgery.
20
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

63%
Bukowska D., Kempisty B., Jackowska M., Wozna M., Antosik P., Piotrowska H., Jaskowski J. M.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
|
nr 2
Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
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