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Polish consumers show increasing interest in “frutti di mare”, including molluscs, which are another potential source of food poisoning. Biotoxins enter the organism of a mollusc through its digestive tract together with plankton, which is its main food, and accumulate in the muscles and hepatopancreas. The main marine biotoxins are diuretic toxins causing diarrhoea, toxins affecting the nervous system, amnesic toxins, and paralytic toxins. Amnesic toxins, causing Amnesic Shellfish Poisoning (ASP), are produced by marine algae such as diatoms and red algae: Chondria spp. Symptoms of ASP in humans occur 24 hours after the consumption of contaminated shellfish in mild poisoning, and from ½ to 6 hours in acute poisoning. The symptoms of poisoning include nausea, vomiting, abdominal cramps, dizziness, headache, confusion, breathing difficulties, and coma. A characteristic symptom of ASP is a usual short-term memory loss. In most cases, neurological symptoms disappear after 48 hours. Some of the symptoms, such as memory problems, may persist even up to 100 days. Mortality is at 4%. The total quantity of amnesic toxins in the whole mussel or any of its edible parts must not exceed 20 mg/kg of mussel meat. Amnesic toxins are detected by chromatographic techniques (HPLC) combined with mass spectrometry and by alternative methods, such as ELISA, phosphatase tests, capillary electrophoresis, and immunochromatography.
Shellfish poisoning is caused by a group of toxins elaborated by planktonic algae (dinoflagellates), in most cases upon which the shellfish feed. The main groups of biotoxins harmful for human beings are: DSP, NSP, ASP and PSP. The most frequent and dangerous for our lives is paralytic shellfish poisoning (PSP). In the case of PSP, the effects are predominantly neurological and include tingling, burning, numbness, drowsiness, incoherent speech, and respiratory paralysis. The 20 toxins responsible for paralytic shellfish poisoning are all derivatives of the saxitoxin group. Saxitoxin is heat-stable and unaffected by standard cooking or steaming; it is water-soluble, and can be concentrated in broth. To prevent outbreaks of PSP and other shellfish intoxications, samples of susceptible mollusks are periodically collected in the coastal states and tested for toxin by mouse bioassay. When toxin levels exceed 80 µg/100 g, affected growing areas are quarantined, and sale of shellfish is prohibited. Modern chemical techniques, for instance immunoassay, phosphatase test or chromatography (HPLC, LC-MS), are quite suitable for the detection of PSP.
The main groups of biotoxins harmful for human are: DSP, NSP, ASP and PSP. Neurotoxic shellfish poisoning (NSP) is caused by consumption of molluscan shellfish contaminated with brevetoxins (BTXs) primarily produced by dinoflagellate, Karenia brevis. In case of intoxication by brevetoxin and their isomers, among the possible effects are paresthesia (tingling), reversal of hot-cold temperature sensation, myalgia (muscle pain), vertigo, ataxia (loss of coordination), abdominal pain, nausea, diarrhea, headache, bradycardia (slow heart rate), and dilated pupils. In total, quantities (measured in the whole body or any edible part separately) cannot exceed the following limit for brevetoxins, i.e. 0.8 mg/kg. Modern techniques such as immunoassay, phosphatase test or chromatography (HPLC, LC-MS) are quite suitable for detection of BTXs.
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Biotoksyny morskie - wystepowanie i metody analizy

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W pracy scharakteryzowano 5 grup biotoksyn morskich występujących w małżach blaszkoskrzelnych (dwuskorupkowych) mogących być przyczyną zatruć po ich spożyciu. Należą do nich toksyny paraliżujące -PSP, wywołujące biegunkę - DSP i AZP, zaburzenia nerwowe - NSP i zaburzenia pamięci - ASP. Spożywanie małż, ostryg i omułków staje się w Polsce coraz popularniejsze, a więc i prawdopodobieństwo zatruć po spożyciu małż wzrasta. Wskazano źródła toksyn morskich, metody ich wykrywania oraz podstawowe akty prawne związanie z hodowlą, dystrybucją i nadzorem nad małżami przeznaczonymi do konsumpcji przez ludzi.
Medycyna Weterynaryjna
|
2010
|
tom 66
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nr 08
s.530-533,bibliogr.
Shellfish poisoning is caused by a group of toxins developed by planktonic algae (dinoflagellates), upon which the shellfish feed in most cases. The main groups of biotoxins harmful for human beings are: DSP, NSP, ASP and PSP. In the case of DSP, the effects include diarrhea, nausea, vomiting and abdominal pain, and it is found in humans very soon after the ingestion of contaminated bivalve mollusks. They also induce tumors of the stomach. Total quantities (measured in the whole mollusks or any edible part separately) should not exceed the following limit for okadaic acid, dinophysistoxins and pectenotoxins together, 160 micrograms of okadaic acid equivalents per kilogram and for yessotoxins, 1 milligram of yessotoxin equivalent per kilogram. Modern chemical techniques such as immunoassay, phosphatase test or chromatography (HPLC, LC-MS) are very suitable for the detection of DSP.
n the last decades, the frequency, intensity and distribution of harmful marine microalgae which produce toxins seem to be increasing. Of the estimated 5 000 living species of algae belonging to phytoplankton, only about 40 species produce toxins. Microscopic toxic algae are an important component of the shellfish diet. This fact together with the expanding seafood industry causes health hazards and great economic losses in different regions all over the world. Consequently, sporadic algal blooms in areas where shellfish are traditionally gathered or commercially farmed require a control system to ensure consumer safety. When humans eat seafood contaminated by marine biotoxins they may suffer a variety of gastrointestinal and neurological illnesses. The longest-known and most infamous group of marine toxins is that responsible for Paralytic Shellfish Poisoning (PSP). The primary neurotoxin is saxitoxin which can cause respiratory paralysis. Classically, the mouse bioassay has been used to detect shellfish toxins. However, efforts have been directed toward the development of a suitable chemical assay for toxicity which would be more sensitive and reproducible. Modern techniques, for instance immunoassay, chromatography, or tissue culture test, are very promising for such an application.
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