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It is a common opinion that main challenge for plant production is to cope with environmental stresses, both abiotic and biotic. Although biostimulator Asahi SL has been used for years to improve plant status and obtain possibly the highest and best quality yield, especially under conditions unfavourable for plant cultivation, its mode of action is still not understood. In this work the effect of Asahi SL on Arabidopsis thaliana plants grown under drought stress was studied. Plants grown under drought stress conditions and treated with Asahi SL were, as compared to untreated, higher and more advanced in development, particularly generative. Biomass accumulation was stimulated by biostimulator mainly due to better photosynthetic apparatus efficiency manifested by a higher (i) assimilation area, (ii) chlorophyll content, (iii) intensity of photosynthesis and (iv) improvement of chlorophyll a fluorescence parameters. Asahi SL treated plants were characterized by a lower concentration of biological active ABA. Despite the higher transpiration and lowered stomatal resistance RWC and water content were unchanged in Asahi SL treated plants which can be explained by increased water uptake in biostimulator sprayed plants. The obtained results clearly showed that the application of biostimulator played a protective role against drought stress.
The aim of research was to define the influence treatment methods involving application of growth biostimulators and herbicide on the total yield of edible potato. The field research was conducted in 2015-2017 in the area of eastern Poland, in Lublin Voivodship. The experiments were performed in three replications using the randomized split-block method. Examined factors were: The examined factors included: I. factor – three early cultivars of edible potato (Owacja, Bellarosa, Vineta), II. factor – treatment methods involving application of growth biostimulators and herbicide: GreenOK-Universal Pro and Asahi SL as well as their combination with the Avatar 293 ZC herbicide. The highest total yield of tubers was obtained from object 4., where prior to sprouting, mechanical treatment was used, and after the final shaping of ridges and just before sprouting the Avatar 293 ZC herbicide was applied at a dose of 1.5 dm3 ha–1, and then after sprouting the GreenOK Universal-PRO was applied three times at doses: 0.10 dm3 ha–1 – full moon-end of seeding + 0.15 dm3 ha–1 – covering inter-rows + 0.15 dm3 ha–1 – formation of flower buds (an average of 41.8 t ha–1) and from the object 5., on which mechanical treatment was used to the seeding, and after the ultimate formation of furrows before seeding of potato plants, Avatar herbicide 293 was applied in a dose of 1.5 dm3 ha–1 (total yield amounted to, on average, 40.6 t ha–1). Potato cultivars cultivated in the experiment and meteorological conditions during the conducted tests also had a significant impact on the size of the total yield. Owacja noted the highest yields (an average of 41.4 t ha–1). The largest total yield was collected in 2016 (an average of 42.8 t ha–1). A significant influence of the experiment factors on the structure of large tuber yields was also found.
The effect of Kelpak® biostimulator, Wuxal® Ascofol and Pentakeep®-V leaf fertilizers were tested on Prunus mahaleb L. ‘Bogdány’ stockplants. Shoot production and characteristics, rooting and quality of their cuttings were evaluated. Biostimulator and fertilizers were sprayed four times on leaves before cuttings were taken. The number and fresh masses of the shoots, cutting weights, leaf chlorophyll indexes (SPAD) and indole3-acetic-acid (IAA) levels in shoot tip were measured on stockplants. Rooting rates, IAA--levels in the rooting zone of cuttings, fresh and dry masses of rooted cuttings, both weights of the root and shoot were measured. Based on these results Kelpak® pretreatment could be considered as the most effective in improving the productivity of Prunus mahaleb L. stockplants, leaf chlorophyll indexes and IAA-level in shoot tip of stockplant and in cutting base. The stockplant pretreatment by Kelpak® increased both the single shoot mass and consequently the fresh mass of prepared cuttings. While these pretreatments alone did not affect the rooting rate, the pretreatment of shoots by Kelpak® and Pentakeep®-V increased the dry mass production of cuttings during rooting. Kelpak® pretreatment affected the root dry mass positively, while Pentakeep®-V increased the shoot dry mass of rooted cuttings.
Growth regulators stimulate life processes in plants, improving their stress resistance and health, which translates into higher and better quality yield. Growth regulators can improve biochemical parameters of tubers and enhance the potato’s resistance to adverse environmental conditions or pathogens. The purpose of this research was to examine the effect of biostimulators on yield and selected chemical properties of potato tubers. Four table potato cultivars were grown in a field experiment: very early Volumia and medium early Irga, Satina and Sylvana. Starting from stage 39 on the BBCH scale (crop cover complete), potato plants were treated thrice, in 10- to 14-day intervals, with the growth regulators Asahi SL, Bio-Algeen S90 and Kelpak. The reference treatment was composed of potatoes untreated with the bioregulators. The growth regulators, especially Bio-Algeen S90 (6.3 – 16.3%) and Kelpak SL (14.2-24.7%) raised the tuber yield, but the effect was statistically verifiable only in the second year, with less precipitation and lower temperature of the vegetation period. The quality of potato tubers was more strongly dependent on the cultivar-specific traits than on the applied biostimulators. In the second year, too, potato tubers contained on average 34% more N-total than in the first year. During storage, the content of N-total in tubers increased by 35-50%. After a five-month storage period, potato tubers contained more NO3 - abut less N-NH4+.
Celem badań było określenie wpływu stosowanych biostymulatorów na plon trzech odmian ziemniaka jadalnego. Doświadczenie polowe przeprowadzono w układzie split-plot w trzech powtórzeniach. Badanymi czynnikami były: I – trzy odmiany ziemniaka jadalnego: Honorata, Jelly, Tajfun, II – pięć rodzajów stosowania biostymulatorów: obiekt kontrolny (bez stosowania biostymulatorów), biostymulator Kelpak SL®, Titanit®, GreenOk®, BrunatneBio Złoto®. Zastosowane w doświadczeniu biostymulatory wpływały na zwiększenie plonu (średnio o 1,6 t·ha–1) w porównaniu do poletka kontrolnego. Przy niekorzystnym układzie warunków pogodowych, niesprzyjającym plonowaniu ziemniaków, po zastosowaniu biostymulatorów uzyskano wyższy plon o 1,2 t·ha–1 niż w warunkach korzystnych. Biostymulator BrunatneBio Złoto wpływał istotnie na zwiększenie i strukturę plonu bulw ziemniaka w porównaniu do obiektu kontrolnego. Uprawiane odmiany różniły się pod względem wielkości plonu. Najwyżej plonowała Jelly (średnio 51,05 t·ha–1) i charakteryzowała się większym udziałem w plonie ogólnym bulw dużych niż odmiany Tajfun i Honorata. Największe plony i procentowy udział bulw dużych uzyskano w warunkach pogodowych 2017 roku, a w pozostałych latach plony były średnio o 1,1-1,2 t·ha–1 mniejsze.
In pot experiments on cucumber cv. Śremski F1, the effect of short-term chilling on plants earlier treated with triacontanol (TRIA) and Asahi SL was investigated. These plants were grown in a phytotron at an air temperature of 27/22ºC (day/night), using fluorescent light with far flux density of 220 μmol x m-2 x s-1, with a photoperiod 16/8. At the 4th true leaf stage, the respective experimental series were sprayed with: 1) H2O – control, 2) TRIA 0.01, 3) TRIA 0.1, 4) TRIA 1.0 mg x dm-3, 5) Asahi SL 0.2, 6) Asahi SL 0.3%. After 24 hours one half of the plants from each experimental series was treated for a period of 3 days at a temperature of 12/6ºC, with all the other growth conditions unchanged. The obtained results have shown that short-term chilling stress caused a significant increase in electrolyte leakage, free proline content and in the activity of guaiacol peroxidase in leaves, but a decrease in chlorophyll a+b content, stomatal conductance, transpiration, photosynthesis, leaf area and in the activity of catalase in leaves. The application of TRIA or ASAHI SL on leaves in the pre-stress period reduced the values of the traits which had been increased as a result of chilling and increased those which had reduced. Generally, TRIA was most effective at a concentration of 0.1 mg x dm-3, and Asahi SL at a concentration of 0.3%.
Four tomato cultivars Macarena F₁, Faustine F₁, Cathy F₁ and Fanny F₁ were used in the study. Transplants were grown in a greenhouse. Seeds were sown at the end of March. The plants were grafted on Maxifort F₁ rootstock on April 5, 6 and 21 in the years 2006, 2007, 2008 without the use of biostimulators. In the case of the other combinations each year the following treatments with biostimulators were performed: watering with Goteo 0.1% solution (twice – 4 and 2 weeks before planting and three times after planting at three-week intervals), spraying with BM 86 0.1% solution (four times every three weeks starting at the blooming of the first cluster). In the control combination plants were untreated with biostimulators and were not grafted. Physical properties were determined and sensory analysis of tomato fruit was performed. The results showed that there was no significant influence of the applied preparations on the a*/b* value of fruits which depended mostly on the cultivars. The highest fruit firmness was obtained with BM preparation and in Faustine F₁ and Cathy F₁ cultivars. There was no significant influence of the preparations on sensory quality of fruits. Variability in sensory quality of fruits rather depended on the analyzed cultivars than on the investigated combination.
In intensive fruit production, in order to obtain high yields, it is necessary to use high fertilization rates as well as plant protection products. An alternative to that kind of production is presented by natural stimulators of plant growth and development called biofertilizers, biopreparations, biostimulators or phytostimulators. They are prepa­rations of natural (plant or animal) origin, harmless to humans and the environment. Biostimulators contain biologically active substances, i.e. plant hormones, enzymes, macro- and microelements, and other compounds that stimulate the growth and development of plants. New approaches to agriculture tend to use environmentally friendly and safe products with a broad spectrum of activity. Nowadays many preparations offered for crop production are designed not only to fertilize the plants and stimulate their growth, but also to protect them from diseases or pests. Phosphite-containing products act as fertilizers or fungicides, and sometimes as biostimulants. "Resistim" is an activating stimulant and fertilizer composed of a potassium phosphite and natural betaines. Vol. 18(1)2010: 111-124 The aim of the study was to evaluate the influence of "Resistim" on the growth and development of three strawberry cultivars (Fragaria x ananassa Duch.). Two of them are short-day cultivars: 'Honeoye' and 'Elsanta', while 'Selva' is a day-neutral cultivar. The experiment was established in October 2008 under controlled conditions in a glasshouse and was carried out for three months. The plants were planted into a mixture of sandy mineral soil and peat in rhizoboxes enabling visualization of root growth and development. The plants were fertilized with standard NPK fertilization (N - 1.02 g, P - 1.9 g, K - 0.78 g per rhizobox), "Resistim" at the dose of 0.2% and 0.4% and NPK (N - 1.02 g, P - 1.9 g, K - 0.78 g) with "Resistim" at the dose of 0.2% and 0.4%, andwatered by an automatic, computer-controlled watering system. Control plants were not fertilized. The data obtained showed significant differences in the responses of the cultivars to "Resistim" treatment 'Honeoye' was the most responsive, although the other two cultivars also responded positively to the treatment with "Resistim". Further field research is required to determine whether the supplemental application of "Resistim" can be beneficial for strawberry fruit production.
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