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Trichoderma is one of the most exploited biocontrol agents for the management of plant diseases. In biocontrol ecology, the critical factors are detection, and the monitoring and recovery of specific biocontrol agents either naturally present or deliberately released into the environment. Protoplast fusion is an appropriate tool for the improvement of biocontrol Trichoderma strains. Protoplast isolation from Trichoderma harzianum was achieved using 24 h culture age, 6.6 mg/ml Novazym L 1412 at 30°C which resulted the maximum protoplast yield of 5 × 108/ml. The self-fused protoplasts were regenerated and 12 fusants were selected based on their growth rate on 2% colloidal chitin medium. Next, a comparison was done for chitinase and antagonistic activity. Transcriptomic analysis based on quantitative real-time RT-PCR, demonstrated that T8-05 fusant expressed 1.5 fold of chit42 transcript as compared with the parental line. This fusant with 7.02±0.15U chitinase activity showed a higher growth inhibition rate (100%) than the parent strain, against Rhizoctonia solani. To obtain a genetically marked fusant that can be used as a biomonitor, the fusant was cotransformed with the gfp and amdS genes. The morphology and viability of selected cotransformant (FT8-7MK-05-2) was similar to the parent. Green fluorescing conidia were observed within the first 2 days of incubation in the soil, and this was followed by the formation of chlamydopores after 60 days. The colonisation of the gfp-tagged fusant was also monitored visually on R. solani sclerotia by scanning electron microscopy. Production of gfp-tagged fusant of Trichoderma spp. provides a potentially useful tool for monitoring hyphal growth patterns and the population of biocontrol agent isolates introduced into environmental systems.
The disturbances in the morphology and anatomical structure of female generative organs were investigated in two species of roadside larch trees Larix decidua Mill., L. decidua subsp. polonica, and L. kaempferi (Lamb.) Carr., within the administrative boundaries of the city of Lublin. The irregularities of female cones appear more often at the juvenile stage of their development. Completely dried cones or cones with partially dried scales were observed on the L. decidua trees growing in a more polluted environment (in comparison with the place of growing of control trees). Among the more developmentally advanced cones, partially dried and completely deformed or bent female generative organs with a disturbed anatomical structure were visible. Peroxidase activity was significant higher in L. decidua subsp. polonica located near the street with heavy traffic. The study can be used as a basis for deeper and more detailed investigations of reproduction of larches in different environments with air pollution in the area of Lublin and its vicinity. Maybe roadside larches and its generative organs can be used as possible biomonitors of air pollution in urban conditions.
Fraxinus excelsior L. (Common Ash) is a deciduous tree that grows in wild and urban Turkey. Fraxinus excelsior has been tested as a possible biomonitor of heavy metals in the city of Kayseri, Turkey. Forty-eight sites (urban road side, road side, urban park, industrial, suburban and rural sites) in and around the city of Kayseri were investigated. In this study, the concentrations of Pb, Cd, Cu, Zn, Ni and Cr in unwashed and washed leaves and soils were determined. There were significant relationships between samples of the heavy metal concentrations from surface soil and washed leaves. The concentrations of heavy metals in the leaves of F.excelsior increased along with the urbanization of the sites. Fraxinus excelsior was found to be a useful biomonitor of the determined heavy metals.
Concentrations of accumulated heavy metals were compared between the terrestrial bryophyte Pohlia nutans and needles and bark of Larix decidua collected on a dump situated in the vicinity of coke- and sulphuric acid-producing factories and a municipal thermal-electric power station. The dump consisted of heavily polluted mine and smelter wastes located near Walbrzych (southwestern Poland). The plant samples were compared with material of the same species from the control site. In the examined area needles and bark can be considered as suitable biomonitors for atmospheric pollution for Co, Cu, Ni and Pb. Bioaccumulation abilities of Pb in P. nutans and L. decidua needles and Ni in P. nutans and L. decidua bark were similar. Compared to each other needles were better accumulators of Ni and the moss P. nutans was a better accumulator of Co and Cu. P. nutans was a better accumulator of Co, Cu and Pb compared to bark.
This overview presents in detail the state of knowledge of the abilities of various components of the Baltic Sea environment to accumulate trace elements and radionuclides. Particular components of the Baltic ecosystem (abiotic and biotic) are considered as potential monitors of pollutants. The use of seaweeds, e.g. Fucus vesiculosus or Zostera marina is recommended, also molluscs, e.g. Mytilus edulis, for biomonitoring surveys of metal pollutants and radionuclides in the Baltic Sea. However, several requirements need to be met if results are to be reliable. Since metal levels and radionuclide activities in the growing tips of F. vesiculosus reflect exclusively the levels of their dissolved species in the ambient seawater, this alga is very useful for monitoring dissolved species of metal pollutants and radioisotopes in the Baltic ecosystem. In contrast, M. edulis, a filter feeder is an appropriate tool for monitoring trace elements occurring in both chemical forms, i.e. dissolved and suspended species. Therefore, full information on the bioavailability and toxicity of heavy metals (depending on their chemical speciation) as pollutants of the Baltic Sea can be obtained if at least two biomonitoring organisms are applied simultaneously, e.g. F. vesiculosus and M. edulis. Moreover, the data matrix can be interpreted more accurately if not only trace element but also macroelement concentrations (Ca, Mg, Na, K) in these two representatives of Baltic phytoand zoobenthos are taken into consideration; this point requires special attention. Two coastal species of fish, i.e. Zoarces viviparus and Perca fluviatilis, are good biomonitors of metallic contaminants, so their use as sentinels is recommended. The budgets of chemical elements and the ecological status of the Baltic Sea are presented. Several ‘blacksp ots’, e.g. large estuaries and seaport towns, heavily polluted by trace elements, are identified in the Baltic Sea and other enclosed seas such the Mediterranean and the BlackS eas. Of these seas, the Baltic is the most heavily loaded with trace elements and organic pollutants. The overview identifies gaps in our environmental knowledge of the Baltic Sea, and sets out possible priorities, key areas or strategies for future research.
Terrestrial moss (Pleurozium schreberi) was used as a biomonitor in multielement studies aimed to estimate the present level of contamination of the Niepołomice Forest environment (S Poland), influenced by industrial emissions since the 1950s. The concentrations of 33 elements (life essential - Na, Mg, Al, Cl, Ca, V, Mn, Fe, Ni, Cu, Zn, Br, Mo, Rb, Cr, As, Se; non-essential elements strongly toxic - Cd, Pb; others - Sc, Sr, Ag, Cs, Ba, La, Tb, Hf, Ta, W, Au, Th, U, Sm) were determined. The results of this study showed that at the end of the 20th century the Niepołomice Forest was an area with average load of industrial emissions in comparison with mean European values. Various sources of elements (long-range and local emissions, crustal/soil dust, vegetation), which influenced their contents in moss, were discussed. It was found that local emissions and crustal/soil dust were the main sources of elements in P. schreberi in the studied area.
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