Wyniki wyszukiwania

1

Application of flow cytometer for molecular identification of Bacillus anthracis

100%

Michalski A. J., Bartoszcze M. A., Zabrocka L. B., Szymajda U.

Żywienie Człowieka i Metabolizm

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2007

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tom 34

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nr 3-4

Flow cytometry is a method of identification biological agents that has various applications. It has been applied for identifying many types of antigens in various materials, including environmental samples. Recently it has been noticed that this method could be used for molecular detection of biological agents. The purpose of this work was to apply flow cytometry with nested-PCR for the molecular identification of B. anthracis. Paramagnetic streptavidin-coated beads were used to capture the resulting fluorophore-labeled sequences. The results show that flow cytometry can be successfully used to detect specific fluorescein- dUTP and a biotin marked sequences.

2

Biological agents database in the armed forces

86%

Niemcewicz M., Kocik J., Bielecka A., Wiercinski M.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 5

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Pegomya nigritarsis Ztt. [Diptera, Anthomyiidae] as an interesting option for biological control of Rumex confertus Willd

86%

Piesik D.

Journal of Plant Protection Research

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2002

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tom 42

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nr 1

There is currently a very favorable climate for biological control of pest weeds. Moreover, biological control plays a significant role in the Integrated Plant Protection Management. Traditional methods of weed control, such as cultivation and the use of herbicides are not practical or desirable for some stands of weeds, and biological control has become an attractive strategy for control of pest weeds. Control of Rumex confertus Willd. by natural enemies that reduce its density is the good example of utilizing biological control on the European and worldwide scale. The aim of the conducted studies was to evaluate the dynamic of population of Pegomya nigritarsis Ztt. (Diptera, Anthomyiidae) on Rumex confertus Willd. and to determine the impact of pest’s injuries on the growth of a plant.

4

Unconventional alternatives for control of tomato root rot caused by Rhizoctonia solani under greenhouse conditions

72%

Hamza A., Mohamed A., Derbalah A.

Journal of Plant Protection Research

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2016

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tom 56

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nr 3

This study was done to assess the antifungal effect of some biocontrol agents effective microorganisms (EMs1), Pseudomonas fluorescences, and Bacillus pumilus, titanium dioxide (TiO2) nanoparticles, black cumin and wheat germ oils as well as the recommended fungicide (flutolanil) against root rot of tomato. Moreover, gas chromatography-mass spectrometry (GC-MS) examination was completed to identify the bioactive compounds in plant oils (dark cumin and wheat germ). Also the impact of these medicines on some biochemical and growth parameters of tomato was examined. Flutolanil was the best treatment followed by dark cumin, TiO2, EMs1, Pseudomonas fluorescens, Bacillus pumilus and wheat germ oil, individually in both test seasons. The outcomes demonstrated a marked increase in each biochemical character (chlorophyll substance, peroxidase and polyphenoloxidase) and plant development (height and fresh and dry weight) under all the tried treatments in comparison to the controls.

5

Basic substances under EC 1107/2009 phytochemical regulation: experience with non-biocide and food products as biorationals

72%

Marchand P. A.

Journal of Plant Protection Research

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2016

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tom 56

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nr 3

Basic Substances are a newly effective category of Plant Protection Product under EC Regulation No 1107/2009. The first approved application of Equisetum arvense L. opened Part C of Implementing Regulation (EU) No 540/2011, which lists the basic substance approved. Although E. arvense was described as a fungicide extract, subsequent applications like chitosan were related to non-biocide molecules. Consequently, plant protection product data were collected from research on alternative or traditional crop protection methods. They are notably issued or derived from foodstuffs (plants, plant by-products, plant derived products, substances and derived substances from animal origin). Applications are currently submitted by our Institute, under evaluation at different stages of the approval process or already approved. Remarkably, this Basic Substance category under pesticide EU Regulation was surprisingly designed for these non-biocidal plant protection products. In fact, components described as the “active substance” of most of the actual applications are food products like sugars and lecithin. Basic Substance applications for these foodstuffs are therefore a straightforward way of easily gaining approval for them. Here we describe the approval context and detail the agricultural uses of theses food products as Biological Control Agents (BCAs) or biorationals for crop protection. From all deposited or approved Basic Substance Application (BSA), a proof has been provided that non-biocide and food products via physical barrier or lure effects may be effective plant protection products with an acceptable low profile of concern for public and agricultural safety.

6

Gastroidea viridula Deg. potential to control mossy sorrel [Rumex confertus Willd.]

72%

Piesik D., Wenda-Piesik A.

Journal of Plant Protection Research

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2005

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tom 45

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nr 2

63-71

Terrain and laboratory research were conducted to determine the potential of Gastroidea viridula Deg. (Coleoptera: Chrysomelidae) to control mossy sorrel (Rumex confertus Willd.). In a field study, the dynamicof plant biomass and number of larvae occurring on that plant were investigated. The Pearson’s linear correlation coefficient of biomass increase in time equalled, to r = 0.96. The regression equation showed, that the plant daily growth reached 29 g; and 210 g per week, consequently. In the laboratory, weight of consumed food by larvae, and larval body weight were measured at 20°C. First generation of G. viridula was taken into consideration. Total weight of consumed leaves by all three instars of a single larva, during 50 days of the development amounted to 1.243 g. Also seasonal abundance of larvae was observed. On May 25th the highest observed number of G. viridula larvae per plant ranged from 435 to 469 individuals. This species may be of usefulness in biological control of mossy sorrel.

7

Health risks due to exposure to biological agents during removal of organic waste. A survey of gaps in knowledge

72%

Van Yperen H. R., Rutten A. L. M.

Annals of Agricultural and Environmental Medicine

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1997

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tom 04

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nr 1

8

The occurrence of Hypera rumicis L. [Col., Curculionidae] on Rumex confertus Willd. as an interesting option for biological weed population control

72%

Piesik D.

Journal of Plant Protection Research

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2001

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tom 41

|

nr 3

Biological methods of weed control are alternative to chemical treatments. Biological techniques are used where no possibility for chemical spraying occurs. Rumex confertus Willd. is an example of a plant-eater which is potentially more important than chemical compounds. The objective of the study was to evaluate population dynamics and the development of Hypera rumicis L. (Coleoptera, Cucurlionidae) on Rumex confertus Willd. as well as to determine effects of the injuries on growth of a plant.

9

Impact of herbicide on mossy sorrel, Rumex confertus Wild., and phytophagous Gastroidea viridula Deg. and Gastroidea polygoni L. [Coleoptera: Chrysomelidae]

72%

Piesik D., Lamparski R.

Electronic Journal of Polish Agricultural Universities. Series Biology

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2004

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tom 07

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nr 2

Field and laboratory research was conducted to determine the effect of five herbicides on Rumex confertus Willd. which is the host of Gastroidea viridula Deg. and Gastroidea polygoni L. (Coleoptera: Chrysomelidae). Herbicide treatment effected plant growth, but new leaf rosettes were produced by the end of the summer. Survival, seasonal abundance and development of investigated insects were significantly reduced. Beetles and larvae avoided feeding on herbicide treated plants.

10

Microbial and endotoxin immissions in the neighborhood of a composting plant

72%

Danneberg G., Gruneklee E., Seitz M., Hartung J., Driesel A. J.

Annals of Agricultural and Environmental Medicine

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1997

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tom 04

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nr 1

11

Exploitation of plant products and bioagents for ecofriendly management of chilli fruit rot disease

58%

Anand T., Bhaskaran R.

Journal of Plant Protection Research

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2009

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tom 49

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nr 2

195-203

Forty four plant species and eight antagonistic organisms were tested against Colletotrichum capsici and Alternaria alternata, the causal agents of fruit rot disease of chilli. In vitro studies indicated that leaf extracts (10%) of Abrus precatorius (Gundumuthu) and Aegle marmelos (vilvum), demonstrated the highest inhibition of spore germination and mycelial growth of these two pathogens. Among the fungal and bacterial antagonists tested, Trichoderma viride isolate 3 and Pseudomonas fluorescens were very effective in inhibiting mycelial growth of the pathogens in vitro. In the pot culture experiment, two sprays with leaf extract of A. precatorius (10%), first spray 20 days after fruit set and the second spray 2 days after inoculation with the pathogens, resulted in the lowest disease incidence (23.95%) and intensity (27.60 PDI - Per cent Disease Index) as compared to 71.50% incidence and PDI of 78.20 in the control. Among the antagonistic microorganisms two sprays of talc-based formulation of P. fluorescens (2%) were very effective in reducing the disease intensity (35.70 PDI). However, the leaf extracts and antagonistic organisms only ranked next to the fungicide (carbendazim 0.1%) (18.05 PDI). Field evaluation of the effective plant extracts, antagonistic microorganisms and fungicide revealed that spraying with A. precatorius leaf extract (10%) twice, the first spray at the time of fruit set and the second spray 20 days after fruit set caused the maximum disease reduction (25.53 PDI) followed by a single spray of the same leaf extract (10%) on 20th day after fruit set (28.50 PDI).

12

Characteristics as well as past and present possibilities of using microorganisms listed on the CDC list A of biological agents in bioterrorist attacks

58%

Weiner M., Tarasiuk K.

Health Problems of Civilization

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2019

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tom 13

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nr 1

The study aims at characterising four bacterial infectious agents listed on the CDC A list, i.e. Anthrax (Bacillus anthracis), plague (Yersinia pestis), botulism (Clostridium botulinum) and tularemia (Francisella tularensis) as potential tools used in a bioterrorist attack causing diseases. The paper also includes information on their occurrence in Poland and the EU. Despite the real threat of terrorism in the 21st century and large-scale activities aimed at limiting the occurrence of this phenomenon, it should be borne in mind that pathogens listed on the CDC list A, although spotted primarily in animals, can be a real threat to people’s health and life. Among the discussed microorganisms, only Francisella tularensis and Clostridium botulinum cause sporadic diseases in Poland; however, it should be remembered that both Bacillus anthracis, occurring in Europe, and Yersinia pestis, occurring in Asia and Africa, can pose real threats to human health and life not only in natural infections, but when used as a biological weapon.

13

Biological agents as occupational hazards - selected issues

58%

Dutkiewicz J., Cisak E., Sroka J., Wojcik-Fatla A., Zajac V.

Annals of Agricultural and Environmental Medicine

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2011

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tom 18

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nr 2

14

Exposure, symptoms and airway inflammation among sewage workers

58%

Heldal K. K., Madso L., Huser P. O., Eduard W.

Annals of Agricultural and Environmental Medicine

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2010

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tom 17

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nr 2

263-268

15

Isolation and evaluation of biocontrol agents in controlling anthracnose disease of mango in Thailand

58%

Rungjindamai N.

Journal of Plant Protection Research

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2016

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tom 56

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nr 3

The agricultural based economy is a core business in Thailand and food export is one of the main sources of income for the Thai population. However, pesticides are overused and misused. As a result there is an urgent need to reduce the use of synthetic chemicals. Biological control offers an alternative to the use of pesticides. Mango (Mangifera indica L.) is widely planted in Thailand and is one of the major cash crops for international export. However, mango suffers from various diseases especially anthracnose, a fungal disease caused by Colletotrichum gloeosporioides. One hundred and twelve isolates of epiphytic microbes were isolated from healthy leaves and fruits of mangoes; this included 93 and 19 isolates of epiphytic bacteria and yeasts, respectively. They were screened for bioactivity against a pathogenic strain of C. gloeosporioides isolated from diseased mangoes using a dual culture technique. Out of 112 isolates, eight isolates exhibited at least 60% inhibition. These isolates were further screened for their inhibition on mango using fruit inoculation. Two isolates reduced the lesion sizes caused by C. gloeosporioides compared to control treatment. These two isolates, based on phenotypical and biochemical tests, were identified as Bacillus sp. MB61 and Bacillus sp. LB72.

16

Correlation between biological agents and levels of heavy metals in municipal sewage sludge

58%

Bozek U., Klapec T.

Annals of Agricultural and Environmental Medicine

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2008

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tom 15

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nr 2

17

The occurrence of Gastroidea viridula Deg. and Gastroidea polygoni L. [Col. Chrysomelidae] on Rumex confertus Willd. as biological representatives of weed population control

58%

Piesik D.

Journal of Plant Protection Research

|

2000

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tom 40

|

nr 3-4

Weeds are harmful organisms connected with human activities; therefore there is a need for their control. Human development and mobility have caused on purpose or purely incidental introduction of plants, exotic very often, to new sites and ecosystems. A dominating method of weed control is applying chemicals. However, chemical compounds are often of low selectivity, they also contaminate the environment and become ineffective quickly because of acquired resistance of treated organisms. The control of Rumex confertus Willd. and other weeds of the genus Rumex spp. is often not possible because of economical reasons. Large areas abundant in sorrel populations would require a large sum of money invested in expensive chemical control. Biological methods of weed control look far more promising solution to this problem. Rumex confertus Willd. is a plant corresponding to criteria to qualify it as an object for biological regulation. The objective of the study was to evaluate dynamic of population and development of the insects of the genera Gastroidea spp. (Gastroidea viridula Deg. and Gastroidea polygoni L.) occurring on Rumex confertus Willd.

Ograniczanie wyników

Application of flow cytometer for molecular identification of Bacillus anthracis

Biological agents database in the armed forces

Pegomya nigritarsis Ztt. [Diptera, Anthomyiidae] as an interesting option for biological control of Rumex confertus Willd

Unconventional alternatives for control of tomato root rot caused by Rhizoctonia solani under greenhouse conditions

Basic substances under EC 1107/2009 phytochemical regulation: experience with non-biocide and food products as biorationals

Gastroidea viridula Deg. potential to control mossy sorrel [Rumex confertus Willd.]

Health risks due to exposure to biological agents during removal of organic waste. A survey of gaps in knowledge

The occurrence of Hypera rumicis L. [Col., Curculionidae] on Rumex confertus Willd. as an interesting option for biological weed population control

Impact of herbicide on mossy sorrel, Rumex confertus Wild., and phytophagous Gastroidea viridula Deg. and Gastroidea polygoni L. [Coleoptera: Chrysomelidae]

Microbial and endotoxin immissions in the neighborhood of a composting plant

Exploitation of plant products and bioagents for ecofriendly management of chilli fruit rot disease

Characteristics as well as past and present possibilities of using microorganisms listed on the CDC list A of biological agents in bioterrorist attacks

Biological agents as occupational hazards - selected issues

Exposure, symptoms and airway inflammation among sewage workers

Isolation and evaluation of biocontrol agents in controlling anthracnose disease of mango in Thailand

Correlation between biological agents and levels of heavy metals in municipal sewage sludge

The occurrence of Gastroidea viridula Deg. and Gastroidea polygoni L. [Col. Chrysomelidae] on Rumex confertus Willd. as biological representatives of weed population control