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Investigation of biofilm formation ability of coagulase-negative staphylococci isolated from ready-to-eat meat

100%

Woroszylo M., Pendrak K., Ciecholewska D., Padzik N., Szewczuk M., Karakulska J.

Acta Scientiarum Polonorum. Zootechnica

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2018

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tom 17

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nr 4

2

Antibiotic susceptibility and biofilm-forming capacity of enterococci isolated from food of animal origin

88%

Duckova V., Canigova M., Krocko M., Lavova M.

Medycyna Weterynaryjna

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2014

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tom 70

|

nr 01

Enterococci are found in the gastrointestinal tract of humans and animals, in soil, and in water, but they also have a long history of use in the production of traditional fermented food. Some strains of enterococci are considered as emerging pathogens of humans. The intrinsic and acquired resistance of enterococci to antibiotics has special significance. Food of animal origin may be a source of resistant enterococci. The main cause of food contamination with enterococci is improper cleaning and disinfection of equipment. The aim of this study was to assess the antibiotic susceptibility of enterococci isolated from different types of food of animal origin and the ability of these microorganisms to form biofilm. Out of a total of 63 isolates, relatively few enterococci were resistant to gentamicin (1.59%). The highest level of resistance was noted for vancomycin (7.94%) and ampicillin (9.52%). However as many as 19.05% and 44.44% of enterococci were resistant to erythromycin and tetracycline, respectively. The ability to form biofilm was detected in 14.29% of the strains of enterococci tested.

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Synergistic antibacterial effect of phenolic acids against Escherichia coli

88%

Synowiec A., Zyla K., Gniewosz M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2019

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tom 598

4

Biofilm and Quorum Sensing in Archaea

88%

Pawlikowska-Warych M., Tokarz-Deptula B., Czuprynska P., Deptula W.

Acta Biologica

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2019

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tom 26

5

Biofilm forming multi drug resistant Staphylococcus spp. among patients with conjunctivitis

75%

Murugan K., Usha M., Malathi P., Saleh Al-Sohaibani A., Chandrasekaran M.

Polish Journal of Microbiology

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2010

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tom 59

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nr 4

Biofilm forming multidrug resistant Staphylococcus spp. are major reservoirs for transmission of ophthalmic infections. They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. Our results indicate that they are genetically close to other community acquired MRSA.

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Electron microscopic examination of dental unit waterlines biofilm

75%

Szymanska J.

Annals of Agricultural and Environmental Medicine

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2005

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tom 12

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nr 2

295-298

Using the transmission electron microscope, the ultrastructural examination was conducted to detect the presence of bacterial biofilm on the inner surfaces of the tubing in dental unit waterlines (DUWL). Samples for examination were taken from the tubes providing water to high-speed and slow-speed handpieces, and to an air-water syringe before application of a disinfection procedure. The microscopic analysis made it possible to find the biofilm in all the tubes in the dental unit which were not pre-disinfected. In these samples, no significant differences were found between high-speed, slow-speed and air-water lines.

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Cationic porphyrin-mediated photodynamic inactivation of Candida biofilms and the effect of miconazole

75%

Davies A., Gebremedhin S., Yee M., Padilla R. J., Duzgunes N., Konopka K., Dorocka-Bobkowska B.

Journal of Physiology and Pharmacology

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2016

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tom 67

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nr 5

8

Antibiofilm activity of commercial water solutions containing copper, silver and gold nanoparticles

75%

Marchwinska K., Gwiazdowska D., Jus K.

Towaroznawcze Problemy Jakości. Polskie Towarzystwo Towaroznawcze

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2018

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nr 3

9

Glass surface as potential in vitro substratum for Candida famata biofilm

75%

Malm A., Chudzik B., Piersiak T., Gawron A.

Annals of Agricultural and Environmental Medicine

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2010

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tom 17

|

nr 1

115-118

10

A novel approach to study the effect of ciprofloxacin on biofilms of Corynebacterium spp. using confocal laser scanning microscopy

75%

Olender A., Bogut A., Magrys A., Krol-Turminska K.

Polish Journal of Microbiology

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2018

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tom 67

|

nr 4

Non-diphtherial corynebacteria are Gram-positive rods that cause opportunistic infections, what is supported by their ability to produce biofilm on artificial surfaces. In this study, the characteristic of the biofilm produced on vascular and urological catheters was determined using a confocal microscopy for the most frequently involved in infections diphtheroid species. They were represented by the reference strains of Corynebacterium striatum ATCC 6940 and C. amycolatum ATCC 700207. The effect of ciprofloxacin on the biofilm produced by the antibiotic-susceptible C.striatum strain was evaluated using three concentrations of the antimicrobial agent (2×, 4×, and 6× the MIC – the Minimum Inhibitory Concentration). The basis for the interpretation of results was the statistical analysis of maximum points readings from the surface comprising a total of 245 areas of the biofilm image under the confocal microscope. It was observed that ciprofloxacin at a concentration equal to 4×MIC paradoxically caused an enlargement of areas with live bacteria within the biofilm. Biofilm destruction required the application of ciprofloxacin at a concentration higher than 6×MIC. This suggests that the use of relatively low doses of antimicrobial agents may increase the number of live bacteria within the biofilm, and further facilitate their detachment from the biofilm’s structure thus leading to the spread of bacteria into the bloodstream or to the neighboring tissues. The method of biofilm analysis presented here provides the original and novel approach to the investigation of the diphtheroid biofilms and their interaction with antimicrobial agents.

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Cellulase inhibits Burkholderia cepacia biofilms on diverse prosthetic materials

75%

Rajasekharan S. K., Ramesh S.

Polish Journal of Microbiology

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2013

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tom 62

|

nr 3

Burkholderia cepacia is an opportunistic pathogen causing infections in patients with cystic fibrosis. Patients with implanted devices are prone to B. cepacia infections due to its ability to grow as biofilms. Knowing the importance of polysaccharides in a biofilm, enzymes that degrade them were targeted as a possible candidate for antibiofilm agents. In this study, the antibiofilm potential of cellulase against B. cepacia biofilms formed on various prosthetic materials was tested. Cellulase exhibited significant antibiofilm activity against B. cepacia without having much action on its growth, thus ruling out the chance of selection pressure and subsequent development resistance.

12

Biosurfactant from Lactobacillus sp. as an antibiofilm agent

75%

Kaur S., Kaur R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

|

nr 3

13

Characterization of Staphylococcus epidermidis isolates cultured from patients with infected hip prostheses

75%

Bogut A., Niedzwiadek J., Strzelec-Nowak D., Plewik D., Mazurkiewicz T., Marczynski W., Olender A.

Health Problems of Civilization

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2018

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tom 12

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nr 2

Background. Coagulase negative staphylococci are at the forefront of etiologic agents of periprosthetic joint infections (PJIs). The purpose of the study was to characterise causative isolates (n=19) of Staphylococcus epidermidis (SE) – with emphasis on their phenotypic and genotypic heterogeneity. Material and methods. The isolates were cultured from multiple samples obtained perioperatively during revision surgery from 14 patients with clinically and/or microbiologically proven PJI. Phenotypic heterogeneity included variations of colony morphologies, drug resistance patterns and/or the capability of the biofilm formation and was verified by the DNA fingerprinting assay. Results. Phenotypic discrepancies were observed between isolates cultured from 5 patients (35.7%). The genotyping assay identified 3 pairs of isolates as unrelated; single pairs were genetically related and indistinguishable. The biofilm production was detected in 17 isolates, among which 5 (29.4%) were proficient biofilm formers harbouring the icaADBC genes. Additionally, one ica-positive isolate produced a moderate, protease-sensitive biofilm. The remaining isolates were moderate biofilm producers among which four developed protease-sensitive biofilms. Conclusions. The majority of PJIs are monoclonal; nevertheless, phenotypic diversity of SE is a frequent phenomenon which can complicate the diagnostic proceeding. Adherence ability is an important pathogenic trait of SE although the chemical composition of the resultant biofilm, its intensity and regulation of development can vary.

14

Hydrophobicity and biofilm formation of lipophilic skin corynebacteria

75%

Kwaszewska A. K., Brewczynska A., Szewczyk E. M.

Polish Journal of Microbiology

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2006

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tom 55

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nr 3

Lipophilic corynebacteria isolated as natural flora of human skin were examined. Among 119 assayed strains 94% presented a hydrophobic cell surface and 75.6 % were able to form biofilms. These attributes, as well as aggregation in liquid media, were statistically connected with each other and promote the developing of biofilms on solid surfaces. This was characteristic of all the lipophilic Corynebacterium species found on human skin that were examined in this study. C. jeikeium and CDC group G2 strains dominated in this population, and they could be responsible for investigated features in the whole lipophilic skin bacterial population. These two groups are the most common coryneform bacteria isolated from nosocomial infections and these attributes most likely promote them to cause opportunistic infections.

15

Neustonic bacteria of Lake Jeziorak Maly [Poland]

75%

Donderski W., Walczak M., Mudryk Z.

Polish Journal of Environmental Studies

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1998

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tom 07

|

nr 3

This paper presents results of research concerning bacteria inhabiting the surface microlayer and subsurface waters of the eutrophic lake Jeziorak Maly. Total number, biomass and share of heterotrophic bacteria was higher in the surface microlayer than in subsurface water. The rate of secondary production of bacteria was higher in the subsurface water. There is a considerably higher percentage of neustonic bacteria showing the ability to degrade different compounds than planktonie ones.

16

In vitro activity of caspofungin against planktonic and sessile Candida sp. cells

75%

Serefko A., Chudzik B., Malm A.

Polish Journal of Microbiology

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2006

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tom 55

|

nr 2

Candida sp. may be regarded as one of the leading etiologic agents of hospital-acquired infections, including those related with the indwelling medical devices, which become colonized by the yeasts, accompanied by biofilm formation. In this paper we assayed in vitro susceptibility to caspofungin of planktonic and sessile cells of nasopharyngeal isolates of Candida sp. Two types of biomaterials were used - silicone elastomer-coated latex urinary Foley catheter and PCV Thorax catheter. The minimal inhibitory concentrations (MIC) of caspofungin for planktonic Candida sp. cells ranged from 0.008 to 0.031 mg/l, while the minimal fungicidal concentrations (MFC) from 0.008 to 0.062 mg/l, with MFC/MIC ratios ≤2. The minimal concentration of caspofungin preventing adhesion process of Candida sp. on both biomaterials ranged from 0.004 to 0.031 mg/l, while preventing biofilm formation from 0.004 to 0.062 mg/l. In contrast, much higher minimal concentrations of caspofungin were needed to eradicate the mature biofilm (0.25 to >8 mg/l). In all cases, drug concentrations depended on the strain and the biomaterial used. Our preliminary data suggest that caspofungin, showing good anti-adherent activity in vitro against Candida sp., appears to be a potential agent rather for prophylaxis of the yeast infections associated with biomaterials but not for their treatment.

17

EDTA as a potential agent preventing formation of Staphylococcus epidermidis biofilm on polichloride vinyl biomaterials

75%

Juda M., Paprota K., Jaloza D., Malm A., Rybojad P., Gozdziuk K.

Annals of Agricultural and Environmental Medicine

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2008

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tom 15

|

nr 2

18

Bacterial decontamination of DUWL biofilm using oxygenal 6

75%

Szymanska J.

Annals of Agricultural and Environmental Medicine

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2006

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tom 13

|

nr 1

The aim of this study was bacteriological assessment of the dental unit waterlines (DUWL) biofilm - concentration and composition of the aerobe and facultative anaerobe bacterial microflora, and evaluation of the influence of a disinfecting product, Oxygenal 6, on the biofilm composition. Tubing fragments were taken from 25 units twice, before and after disinfection, and bacterial suspension of the biofilm was obtained from the samples. The bacterial flora was determined with the plate culture method. Bacteria were identified with biochemical microtests: API 20E, API 20NE (bioMerieux, France) and GP2 MicroPlateTM (BIOLOG, USA). Before disinfection, the following bacteria were identified: Gram-negative bacteria - Ralstonia pickettii, Pseudomonas vesicularis, Sphingomonas paucimobilis, Xanthomonas maltophilia; Gram-positive cocci - Micrococcus luteus, Micrococcus lylae, Staphylococcus cohnii, Staphylococcus lentus, Staphylococcus spp., Streptococcus spp.; Actinomycetes - Streptomyces albus. The prevailing bacteria were: Ralstonia pickettii (78.62%), found in all the units, and Sphingomonas paucimobilis (20.45%). After DUWL disinfection, Sphingomonas paucimobilis (88.79%) dominated in the biofilm, Staphylococcus spp. - 5.61% and Pseudomonas spp. - 3.74% were next most frequently occurring bacteria, and in more than a half of the biofilm samples 100% reduction of the bacterial microflora occurred. This study confirms effectiveness of Oxygenal 6 in bacterial decontamination of the DUWL biofilm.

19

Interactions between potentially pathogenic fungi and natural human microbiota

63%

Goralska K.

Annals of Parasitology

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2014

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tom 60

|

nr 3

The human body is composed of 1014 cells, of which only 10% of them belong to the human host itself: the remaining 90% are microorganisms. Commensal microorganisms are necessary for the proper functioning of the human body and covers an area that could potentially become sites of adhesion of pathogenic microorganisms, it thus represents a form of competition for potential pathogens. The coexistence of fungi and bacteria in cases of systemic infections is a significant diagnostic and therapeutic problem, and the human immune system reacts differently, depending on the pathogen. Numerous publications exist concerning the relationship between microorganisms belonging to different ecological groups, the majority of which concern the interaction between macro-organisms and potential pathogens, or the synergistic relationship between parasitic species. However, there is still too little information concerning the role of natural microbiota in maintaining homeostasis and the relationships between particular species inhabiting the human organism.

20

Importance of eps genes from Bacillus subtilis in biofilm formation and swarming

63%

Nagorska K., Ostrowski A., Hinc K., Holland I. B., Obuchowski M.

Journal of Applied Genetics

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2010

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tom 51

|

nr 3

Unicellular organisms naturally form multicellular communities, differentiate into specialized cells, and synchronize their behaviour under certain conditions. Swarming, defined as a movement of a large mass of bacteria on solid surfaces, is recognized as a preliminary step in the formation of biofilms. The main aim of this work was to study the role of a group of genes involved in exopolvsaccharide biosynthesis during pellicle formation and swarming in Bacillus subtilis strain 168. To assess the role of particular proteins encoded by the group of epsI-epsO genes that form the eps operon, we constructed a series of insertional mutants. The results obtained showed that mutations in epsJ-epsN, but not in the last gene of the eps operon (epsO), have a severe effect on pellicle formation under all tested conditions. Moreover, the inactivation of 5 out of the 6 genes analysed caused total inhibition of swarming in strain 168 (that does not produce surfactin) on LB medium. Following restoration of the sfp gene (required for production of surfactin, which is essential for swarming of the wild-type bacteria), the sfp⁺ strains defective in eps genes (except epsO) generated significantly different patterns during swarming on synthetic B medium, as compared to the parental strain 168 sfp⁺.

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