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  1. 3 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

  2. 2 Acta Biochimica Polonica

  3. 2 Journal of Horticultural Research

  4. 2 Polish Journal of Microbiology

  5. 1 Acta Scientiarum Polonorum. Hortorum Cultus

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  1. 1 Abbasi U.

  2. 1 Ahmad R.

  3. 1 Alvarez de Morales P.

  4. 1 Anielska-Mazur A.

  5. 1 Bista M.

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  1. 1 2021

  2. 2 2019

  3. 2 2018

  4. 1 2017

  5. 1 2014

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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Iron-superoxide dismutase from pepper: biochemical characterization, cell localization and tissue distribution

100%
Rodriguez-Ruiz M., Alvarez de Morales P., del Rio L., Corpas F., Palma J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
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tom 94
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nr 2
2
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Nanoantibodies: small molecules, big possibilities

100%
Pedreanez A., Mosquera-Sulbaran J., Munoz N., Tene D., Robalino J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2021
|
tom 102
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nr 3
3

Effects of dietary supplementation with polyunsaturated fatty acids and antioxidants on biochemical characteristics of boar semen

100%
Strzezek J., Fraser L., Kuklinska M., Dziekonska A., Lecewicz M.
Reproductive Biology
|
2004
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tom 04
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nr 3
4
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Postharvest calcium salt treatment of fresh jujube fruit and its effects on biochemical characteristics and quality after cold storage

84%
Moradinezhad F., Ghesmati M., Khayyat M.
Journal of Horticultural Research
|
2019
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tom 27
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nr 2
5
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Functional and biochemical characterization of Arabidopsis calcium sensor SCS a potential regulator of SnRK2 protein kinases

84%
Bucholc M., Goch G., Ciesielski A., Anielska-Mazur A., Dobrowolska G.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
|
nr 2
6

A novel member of the thermolysin family, cloning and biochemical characterization of metalloprotease from Staphylococcus pseudintermedius

84%
Wladyka B., Bista M., Sabat A. J., Bonar E., Grzeszczuk S., Hryniewicz W., Dubin A.
Acta Biochimica Polonica
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2008
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tom 55
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nr 3
525-536
Thermolysins constitute a family of secreted bacterial metalloproteases expressed, among others, by several pathogens. Strains of Staphylococcus pseudintermedius isolated from diseased dogs and judged as protease-positive, by skim milk agar plate culture, were investigated for protease content. No proteolytic activity was detected when the bacteria were grown in regular liquid media. Unexpectedly, supplementation of the medium with calcium ions resulted in expression of a metalloprotease and profound changes in the profile of extracellular proteins. On the basis of homology to other staphylococcal metalloproteases, the nucleotide sequence of the gene encoding this protease (Pst) and its flanking regions was determined. The full-length pstcodes for a protein with an open reading frame of 505 amino acids. The internal region contains the HEXXH catalytic domain that is conserved in members of the thermolysin family. Regardless of the presence of calcium in the medium, the expression of the protease gene was of the same intensity. This suggests that regulation of the metalloprotease production by calcium ions is at a post-transcriptional level. Isolates of S. pseudintermediusexhibit a proteolytic phenotype due to the metalloprotease expression, however only in presence of calcium ions, which most probably stabilize the structure of the protease.
7

Phylogenetic and biochemical characterization of a new halo-thermotolerant, biofilm-forming Bacillus from saline lake of Iran

84%
Safary A., Moniri R., Mirhashemi S. M., Nikzad H., Khiavi M. A.
Polish Journal of Microbiology
|
2013
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tom 62
|
nr 4
In this study, five halotolerant Bacillus isolates from Aran-Bidgol Saline Lake in Iran were identified from saline environments. Screening of the bacteria led to the identification of a unique halo-thermotolerant Bacillus. On the basis of genetic and phenotypic data, this isolate was closely related to Bacillus licheniformis. But isolated Bacillus can be distinguished from B. licheniformis by salt tolerance, 16SrDNA sequence and some different physicochemical properties. Thus, suggested that the isolate was not the known Bacillus. Optical density analysis indicated strong biofilm formation for this strain. Also this isolate exhibited average tolerance to 1-25 mM concentrations of zinc and was sensitive to all concentrations of nickel. In biosurfactant production assay, this Bacillus exhibited the high activity for semi-quantitative oil displacement test (3.14 ±0.02 cm²) and evaluated positive for drop-collapse test and hemolytic activity. Moreover, amylase, protease and DNase enzymes produced in presence of 10-20% salt of medium. Therefore, identified Bacillus could supply potential microbial materials for bioremediation purposes and biotechnological applications.
8
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Biochemical characterisation of the tissue degrading enzyme, collagenase, in the spined soldier bug, Podisus maculiventris (Hemiptera: Pentatomidae)

84%
Ghamari M., Hosseininaveh V., Darvishzadeh A., Talebi K.
Journal of Plant Protection Research
|
2014
|
tom 54
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nr 2
Podisus maculiventris (Say) is a generalist predator attacking many insect species from different orders. The bug injects saliva into its prey's body. The ingested hemolymph and liquefied internal tissues pass through the bug's alimentary tract. Collagenase working on peptide bonds of collagen and basement membrane proteins, leads to the disintegration of the prey's internal organs. As yet, there is an almost complete lack of knowledge on the collagenase activity in P. maculiventris. The collagenase activity of the salivary glands and midgut was optimum at pH 8.0 which was congruent with the optimal pH of the total proteolytic activity of the salivary glands. More collagenolytic activity was determined in the posterior lobe of the salivary glands and anterior midgut. Significant inhibition of collagenolytic activity by ethylenediaminetetraacetic acid (EDTA) revealed the enzyme is a metalloproteinase. The collagenase activity notably decreased when the bug went hungry. The salivary gland collagenase is a vital enzyme in extra-oral digestion and facilitates the action of other digestive enzymes. The midgut collagenase may be involved in the digestion of the ingested muscle fibers. The collagenase probably acts as an intoxicating agent in the saliva (venom) of P. maculiventris. Paralysing toxins are present in the salivary gland secretion.
9
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Silica nanoparticles enhances physio-biochemical characters and postharvest quality of Rosa hybrida L. cut flowers

84%
El-Serafy R. S.
Journal of Horticultural Research
|
2019
|
tom 27
|
nr 1
10
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Biochemical, mineral and anatomical characteristics of the olive tree cv. Chetoui growing in several Tunisian areas

67%
Mansour-Gueddes S. B., Saidana D., Cheraief I., Dkhilali M., Braham M.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2018
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tom 17
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nr 2
To tolerate harsh climatic conditions, olive tree Chetoui has developed some anatomic, physiologic and biochemical mechanisms. The aim of this study was to determine the indicators of stress in leaves, stems and roots growing under various climatic conditions. To protect against stress conditions this cultivar increased cuticle thickness, protective structures and building parenchyma tissues of leaves, woods and roots from the North to the South. The volatile compounds, extracted from northern and southern Chetoui leaves and roots, were analyzed by GC-FID and GC-MS. Great changes in volatiles were illustrated in the studied organs, by enrichment in phenolics and fatty acids for leaves and in hydrocarbons for roots of southern Chetoui. Also, a reduction in terpenes, alcohols and carbonylic compounds was noted in both southern samples. Moreover, minerals of all organs of Chetoui, varied in content and allocation, but their levels are the highest in leaves. The changes in volatiles might be affected by changes in the mineral elements uptake or accumulation under environment stress. A significant correlation was noted between phenolic compounds and sodium, nitrogen, and calcium contents. However, terpenoids was highly correlated with phosphorus content for all organs and studied areas. The detection of new volatiles, anatomical and mineral changes seem to be efficient indicators of adaptation of Chetoui to environment stress conditions.
11

Analysis of cultivable aerobic bacteria isolated from bottom sediments in the Wijdefjorden region, Spitsbergen

67%
Konieczna I., Wojtasik B., Kwinkowski M., Burska D., Nowinski K., Zarnowiec P., Kaca W.
Polish Polar Research
|
2011
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tom 32
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nr 2
12

Biochemical characterisation of chlorophyllase from leaves of selected Prunus species - A comparative study

67%
Sytykiewicz H., Sprawka I., Czerniewicz P., Sempruch C., Leszczynski B., Sikora M.
Acta Biochimica Polonica
|
2013
|
tom 60
|
nr 3
Despite senescence-induced chlorophyll depletion in plants has been widely studied, the enzymatic background of this physiologically regulated process still remains highly unclear. The purpose of this study was to determine selected biochemical properties of partially purified fractions of chlorophyllase (Chlase, chlorophyll chlorophyllido-hydrolase, EC 3.1.1.14) from leaves of three Prunus species: bird cherry (Prunus padus L.), European plum (Prunus domestica L.), and sour cherry (Prunus cerasus L.). Secondarily, this report was aimed at comparing seasonal dynamics of Chlase activity and chlorophyll a (Chl a) content within investigated plant systems. Molecular weight of native Chlase F1 has been estimated at 90 kDa (bird cherry) and approximately 100 kDa (European plum and sour cherry), whereas molecular mass of Chlase F2 varied from 35 kDa (European plum) to 60 kDa (sour cherry). Furthermore, enzyme fractions possessed similar optimal pH values ranging from 7.6 to 8.0. It was found that among a broad panel of tested metal ions, Hg+2, Fe+2, and Cu+2 cations showed the most pronounced inhibitory effect on the activity of Chlase. In contrast, the presence of Mg+2 ions influenced a subtle stimulation of the enzymatic activity. Importantly, although Chlase activity was negatively correlated with the amount of Chl a in leaves of examined Prunus species, detailed comparative analyses revealed an incidental decrement of enzymatic activity in early or moderately senescing leaves. It provides evidence that foliar Chlase is not the only enzyme involved in autumnal chlorophyll breakdown and further in-depth studies elucidating this catabolic process are required.
13

Biochemical characterization of podzolic soil under willow culture after three years from its amendment with sewage sludge

67%
Joniec J., Furczak J.
Polish Journal of Soil Science
|
2007
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tom 40
|
nr 2
207-215
The study was conducted on a podzolic soil (originating from a field experiment), fertilized with various doses of municipal-industrial sewage sludge, i.e. 30 (1%), 75 (2.5%), 150 (5%), 300 (10%) and 600 Mg ha⁻¹ (20%), and then planted with willow (Salix viminalisL.). In the third year from setting up the experiment, in two layers of the soil (0-20 and 20-40 cm) determinations were made on the effect of the applied sludge on the respiratory activity, cellulose mineralization rate, intensification of ammonification and nitrification, and on dehydrogenases and protease activity. The results obtained showed a positive effect of the sludge on almost all of the analysed biochemical parameters, both in the surface and in the deeper layers of the soil. The effect of the sludge in the Ap horizon was more pronounced and generally increased with increasing dosage. Only the process of ammonification was subject to inhibition that was stronger in the surface horizon of the soil.
14

Biochemical characteristics of myofibrillar proteins of the fish parasite Isoparorchis hypselobagri (Trematoda) as survival traits in an oxygen-rich environment

67%
Abbasi U., Ahmad R., Hasnain A.-U.
Biological Letters
|
2012
|
tom 49
|
nr 1
15

Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions

67%
Dlugolecka A., Cieslinski H., Bruzdziak P., Gottfried K., Turkiewicz M., Kur J.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 3
211-218
In this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties. However, the differences concerning thermal activity were observed. The optimal temperature for recombinant esterase in the presence of urea (1 M) was about 15°C lower in comparison with enzyme isolated from the native source. Furthermore, the EstA was found to be more thermolabile in denaturant conditions. The differences were presumably caused by slightly changed protein structure in the presence of urea. The preservation of activity of EstA dissolved in buffer containing 8M urea suggests that the protein structure is retained and it does not undergo dramatic changes due to high urea concentration. This thesis was confirmed with FT-IR data.
16
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Extension of shelf-life of tomato using irradiated chitosan and its physical and biochemical characteristics

67%
Parvin N., Kader M. A., Huque R., Molla M. E., Khan M. A.
International Letters of Natural Sciences
|
2018
|
tom 67
The effect of irradiated chitosan coating on post-harvest preservation of tomato was observed in this study. Irradiated chitosan (40 kGy) solution of various concentrations (500, 750, 1000, 1500 and 2000 ppm) were applied on post-harvest preservation of tomato. Both chitosan treated and untreated (control) tomato were stored at room temperature in open and zip bag conditions. The effect of coating of various chitosan solutions on tomato was observed during storage period. The percentage of weight loss and spoilage rate of the preserved and control tomato samples were investigated. Several parameters (such as total bacteria count, total mold count, moisture, ash, acidity, vitamin C, sugar, protein and fat) were analysed for irradiated chitosan coated tomato in open condition after 3-weeks storage period. In addition, the same parameters were also analysed for control tomato. Considering all parameters, the results revealed that 1500 ppm chitosan solution performed better in extending the shelf-life of tomato as compared to the control and other treated samples. Thus, this observation recommend that irradiated chitosan coating have the potential to be used as natural preservative to maintain quality and extending shelf-life of tomato.
17

Biochemiczna charakterystyka izolatow bakterii raka pomidora [Clavibacter michiganensis subs.michiganensis]

59%
Kamasa J., Pospieszny H.
Progress in Plant Protection
|
2002
|
tom 42
|
nr 2
773-776
18

Evaluation of European Cranberrybush (Viburnum opulus L.) genotypes for agro-morphological, biochemical and bioactive characteristics in Turkey

51%
Ersoy N., Ercisli S., Gundogdu M.
Folia Horticulturae
|
2017
|
tom 29
|
nr 2
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