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A Yarrowia lipolytica JII1c yeast strain, isolated from the Polish ‘Rokpol’ mould cheese, was used as an adjunct culture in the production of a Dutch-type cheese. Its effect on the microbiological and biochemical characteristics of the cheese was evaluated in this research study. Milk used to produce the cheese was inoculated with 105 cfu/mL yeast cells. During the ripening process, the yeast population grew systematically to reach a maximum level of 7.9 log cfu/g in the sixth week of maturation, whereas the number of lactic acid bacteria increased until the fourth week of ripening. Thereafter, the number of microorganisms in the both groups decreased. After 8 weeks of ripening, the pH value of cheese inoculated with yeasts was significantly higher than that of the control cheese sample (produced without those microorganisms) and reached the levels of 6.37 and 5.47, respectively. In the experimental cheeses, it was also found that the utilization rate of lactic and citric acids was higher. Additionally, the concentration levels of water-soluble nitrogen (WSN) and free amino groups (FAG) in the experimental cheeses were about twice as high as in the control cheese sample. A more intensive proteolysis in the experimental cheese was accompanied by a higher accumulation of biogenic amines, especially of tyramine, putrescine, and 2-phenylethylamine; in the experimental cheese, after 8 weeks, their contents amounted to: 167.01, 77.90, and 69.54 mg/100 g, respectively. In contrast, the concentration of histamine was similar in both cheeses (9.47 and 9.81 mg/100 g in the control and experimental cheese samples, respectively). Also, the experimental cheese revealed more pronounced lipolysis resulting in a higher accumulation of free fatty acids, especially of butyric, myristic, palmitic, stearic, and oleic acids. It can be concluded that the Y. lipolytica JII1c grew well in the cheese causing the ripening process of the cheese to significantly accelerate.
Chloramine-T is a widely used disinfectant for the treatment of gill diseases of fish in freshwater, and more recently attention has turned to its use in seawater. However, despite the wide use of chloramine-T, few studies have examined its toxicity to fish. Therefore, the aim of the current study was to examine the effects of disinfection by Chloramine-T on the muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum) using oxidative stress biomarkers (levels of 2-thiobarbituric acid reactive substances and derivatives of oxidatively modified proteins) and biochemical enzymes’ activity (alanine- and aspartate aminotransferases (ALT and AST), lactate dehydrogenase (LDH)) to observe the its toxic effects. The endpoints obtained from this study will be useful to monitor the effects of disinfectant bathing with Chloramine-T for this species of fish. In the disinfectant group, rainbow trout (n = 11) were exposed to Chloramine-T in final concentration of 9 mg per L. Control group of trout (n = 11) was handled with water from basin in the same way as Chloramine-T exposed group. Fish were bathed with Chloramine-T for 20 min and repeated three times every 3 days. Two days after the last bathing fish were sampled to study. Our results showed that Chloramine-T bathing caused the decrease of the lipid peroxidation as well as ALT and AST activity and significant decrease of LDH activity (by 339%, p = 0.017) compared to controls. Chloramine-T markedly affects on lactate and pyruvate metabolism and resulted to decrease of LDH activity. Correlative analysis revealed that the lipid peroxidation level is correlated with ALT and AST activity in the muscle tissue of unhandled control group. In the muscle tissue of trout disinfected by Chloramine-T, LDH activity is correlated positively with ALT and AST activity. Thus, the skeletal muscles of fish play an important role in the processing of lactate through the gluconeogenic and glycogenic pathways including a greater potential for biosynthesis. Our studies indicated that Chloramine-T in dose of 9 mg per L could at least partly attenuate oxidative stress and can be used for prophylactic disinfecting treatment of rainbow trout. Oxidative stress and biochemical alterations could be effectively used as potential biomarkers of Chloramine-T toxicity to the fish in the warning signal for pharmaceutical exposure to aquatic organisms. However, more detailed studies on using of these specific biomarkers to monitor the disinfectant treatment in aquaculture are needed.
Increasing concentrations of chromium caused reduction in growth, chlorophyll contents and mineral mutrients of bhendhi. At increasing concentrations of chromium, all the attributes were found to be reduced. Chromium is one of the toxic elements discharging from tannery industry. The long disposal of tannery effluent to the agricultural land causes serious damages in the soil texture; reduce the growth of microorganisms and agricultural crops. Large areas of Vellore district of Tamilnadu was seriously affected by the tannery effluent irrigation to the agricultural land. The present study was conducted to identify the phytotoxic effect and physiological changes of Abelmoschus esculentus (L.) Moench under the treatment of different concentrations of chromium solutions (2.5, 5, 10, 25, 50, 75 and 100 mg/l). The result shows that there was a gradual reduction in the morphological parameters (root length, shoot length, fresh weight and dry weight). Similarly the photosynthetic pigments (Chlorophyll and carotenoid) and biochemical parameters such as protein, amino acid, and sugars were also gradually decreased with increasing concentrations of chromium.
Seed priming is one of the potential physiological approaches to enhance the seed germination under the salinity stress. The present study examined the role of two seed priming molecules: salicylic acid (SA) and hydrogen peroxide (H2O2), in enhancing the salt tolerance of lentil seeds at germination stage. Salinity stress caused significant decrease in germination percentage and primary root elongation. This decrease was associated with significant increase in lipid peroxidation and total lipid (TL) contents in embryonic axis. The catalase (CAT), guaiacol peroxydase (GPOX) and superoxide dismutase (SOD) activities remained unchanged or decreased significantly under the influence of salt stress, in both embryonic axis and cotyledons. Starch mobilization was not affected by the salt stress. The two priming treatments effectively alleviated the negative effects of salinity stress. SA and H2O2 applications after dose optimization resulted in a significant enhancement of germination percentage and primary root elongation. No significant changes in starch, soluble sugars contents and SOD activity were detected following SA and H2O2 treatments. Seed priming treatments triggered the activities of GPOX and CAT and caused the reduction in lipid peroxidation, especially in embryonic axis. TL content and especially the fatty acid C18:3 increased after SA applications. Better performance under salt stress of primed lentil seeds was associated with lower lipid peroxidation, and activation of enzymatic antioxidative defense system. Obtained results confirm the potential for using SA and H2O2 to improve germination and plant growth under salt stress conditions.
Sugar mill is one of the agro based industry and great significant in rural economy of developing countries. It creates environmental pollution by produced waste during sugar production. In the present work deals with the analysis of various change of biochemical in African marigold (Tagetes erecta L.) at 15 DAS under the different concentrations (control, 10, 25, 50, 75 and 100%) of sugar mill effluent. Results of this study determined that the lower concentration (10%) of sugar mill effluent increased photosynthetic pigment and biochemical contents of African marigold and it decreased at higher concentrations of the sugar mill effluent. The lower concentrations of sugar mill effluent used for irrigation.
The relationship between growth and some enzymes of carbohydrate metabolism in developing cotton fibre were studied. Two respiratory pathways of glucose oxidation i.e. oxidative pentose phosphate pathway (OPPP) and glycolysis operates in the elongating cotton fibres and the extent of their operation varies with the demand for respiratory products. In this respect, hexokinase, G-6-PDH, 6PGDH, and MDH show increased activities during the period of rapid cell elongation and decreased activities when rate slows down. The conversion of PEP to malate and/or via a transhydrogenase system consisting of enzymes PEPC, MDH and NADP-MDH(d) may play a significant role in carbohydrate compartmentation of developing cotton fibre. As the rate of fibre growth slows down, a decline in enzyme activities, points to a shift in metabolic priorities.
We show that treating human trisomie fibroblasts with anthracyclines - aclarubicin, daunorubicin and idarubicin - leads to certain changes in these cells; namely the activation of caspase 3, morphological changes and an increase in the level of intracellular calcium. These results suggest that anthracycline drugs are also able to induce apoptosis in pathological, trisomic cells.
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