Wyniki wyszukiwania

1

Ultrasonographic examination of gallbladder contractility in the sheep

100%

Romanski K. W., Siembieda J.

Polish Journal of Veterinary Sciences

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1998

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tom 01

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nr 1

To develop a new animal model for gallbladder ultrasonography 16 adult sheep of both sexes were used. Ultrasonographic examinations were performed in fed or fasted sheep every 30 min for 3-5 h. Every gallbladder was visualized, then its maximal length, height and width were measured. Afterwards, the gallbladder volume was calculated with three methods. The maximal and minimal values of gallbladder volume in both fed and fasted animals differed significantly (p < 0.05) indicating the existence of gallbladder contractions detectable in both states examined. The results of gallbladder volumes calculated with various methods were also significantly different. The values obtained from the method describing the gallbladder as a cone were about 50% lower than that obtained from two ellipsoid methods. The validation study showed that the true value remained somewhere between the values obtained indirectly. This warrants further investigation, however.

2

Gastroenterological research in domestic animals - past, present and future perspectives

84%

Zabielski R., Gill J.

Journal of Physiology and Pharmacology. Supplement

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2003

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tom 54

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nr 3

263-281

The gastrointestinal research in domestic animals in Poland is briefly discussed in the section. The history starts over seventy years ago with the creation of the Department of Animal Physiology at the Veterinary Faculty of Warsaw University. Professor B. Gutowski, the first head of the Department, and his pupils established the School of Gastrointestinal Physiology; renowned for the achievements in physiology of digestion, gastrointestinal motility, pancreas and liver functions, and comparative physiology of domestic ruminants and wild animals. After the WWII the gastrointestinal research has also been initiated in the newly established faculties of veterinary and animal science of the agricultural universities in Lublin (motility, composition of pepsinogen, biliary and pancreas secretion, vitamin and microelement absorption), Szczecin (lipid absorption, lymph formation), Wroc³aw (gastrointestinal and gall bladder motility, bile secretion) as well as in the Institute of Animal Physiology and Nutrition of the Polish Academy of Science (digestion and absorption, development of the gastrointestinal tract in neonates). The research activity was focused on solving the problems faced by animal production in Poland, but it also resulted in a considerable number of physiological findings of an international dimension, and initiated new research areas.

3

The short history of gastroenterology

84%

Srodka A.

Journal of Physiology and Pharmacology. Supplement

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2003

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tom 54

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nr 3

9-21

In this paper research on the stomach and bowel physiology is presented in a historical perspective. The author tries to show how digestive processes were interpreted by the ancients and how they tried to adjust them to the dominating humoral theory of disease. It is pointed out that the breakthrough which created a new way of understanding of the function of the digestive system was made by Andreas Vesalius and his modern model of anatomy. The meaning of acceptance of chemical processes in digestion by iatrochemics representatives in XVII century is shown. Physiological research in XIX century, which decided about a rapid development of physiology, especially the physiology of the gastrointestinal tract, is discussed. Experiments were performed by all main representatives of this discipline: Claude Bernard, Jan Ewangelista Purkynì, Rudolph Heidenhain and especially Ivan Pavlov, who, thanks to the discoveries in the secretion physiology, explained basic functions of the central nervous system. The XX century was dominated by the research showing the important role of the endocrine system and biological agents in the regulation of secretion and motility of the digestive system. The following discoveries are discussed: Ernest Sterling (secretin), John Edkins (gastrin) and André Latarjet and Lester Dragstedt (acetylcholine). It is underlined that Polish scientists play an important role in the development of the gastroenterological science - among others; Walery Jaworski, who made a historical suggestion about the role of the spiral bacteria in etiopathogenesis of the peptic ulcer, Leon Popielski, who stated the stimulating influence of histamine on the stomach acid secretion, Julian Walawski, who discovered enterogastrons - hormones decreasing secretion. As a supplement, there is the list of achievements in the field of the physiology and pathology of the gastrointestinal tract awarded with Nobel Prize and the list of most important Polish papers in this field.

4

Evidence for probiotic potential of a capsular-producing Streptococcus thermophilus CHCC 3534 strain

84%

Khalil R.

Polish Journal of Microbiology

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2009

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tom 58

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nr 1

49-55

The purpose of this research was to evaluate the probiotic potential of an capsulated Streptococcus thermophilus CHCC 3534 strain. The strain tolerates 0.4% oxgall (bile) and was sufficiently resistant to pH as low as 2.5 for 3 hours of exposure. The strain demonstrated high adherence to human intestinal mucus, and showed unique resistance to different antibiotics. Crude extracts of S. thermophilus CHCC 3534 contained a diffusible antimicrobial compound "bacteriocin" with a broad spectrum that inhibited the growth of closely related lactic acid bacteria and a number of food spoilage bacteria including Salmonella typhimurium and Staphylococcus aureus. The bacteriocin was heat stable, resistant to pH, inactivated by proteolytic enzymes, and resistant to á-amylase and lipase. A SDS-PAGE analysis of the partially purified bacteriocin revealed one component with a molecular weight ranging from 14.4 to 18.4 kDa. The strain may have industrial significance and represents an interesting candidate for use in biopreservation, probiotic food formulations and in the control of spoilage caused by food borne pathogens.

5

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Secretin, cholecystokinin and the biliary canalicular secretion in the rat

84%

Romanski K. W., Bochenek W. J., Unold J., Szczepaniak M.

Journal of Physiology and Pharmacology

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1995

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tom 46

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nr 2

6

Survival of kefir bacteria in an acid environment and in the presence of bile

84%

Usajewicz I.

Polish Journal of Natural Sciences

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2002

|

tom 11

7

High performance liquid chromatography analysis of reduced glutathione in biological materials - report

84%

Udeh K. O., Achremowicz B.

Polish Journal of Food and Nutrition Sciences

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2001

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tom 10

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nr 1

A reverse-phase HPLC spectrofluorometric method was developed for routine, specific and fast analysis of glutathione (GSH) in biological materials based on modified O-opthaldialdehyde derealization procedures. The developed method allowed the determination of glutathione in yeast biomass, bile and plasma samples from goats. The limit of detection was estimated to be 8 µМ for yeast biomass at a signal to&noisc ratio of 1 and was further extended to signal ratio of 2, which allowed the detection of lower amounts of GSH in the bile and plasma up to 10 ng/cm3 The methods ol GSH extraction from biological materials, derivatization procedures and separation by HPLC method were reported.

8

Wydalanie siarki pojedynczej i podwojnej z zolcia krolikow zatruwanych dwusiarczkiem wegla

67%

Gregorczyk J., Kosmider K., Wesolowska T., Sawrymowicz M., Piotrowski P.

Bromatologia i Chemia Toksykologiczna

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1999

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tom 32

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nr 3

297-301

Badania nad metabolizmem dwusiarczku węgla (CS2) w organizmie zwierząt wykazały, że stopień retencji zarówno wolnego, jak i związanego CS2 jest w wątrobie znaczny i to bez względu na drogę podawania (1, 2). Jak dotychczas nie znane są dalsze losy metaboliczne kumulowanego związku. Duża aktywność wątroby pozwala przypuszczać, że pewna ilość metabolitów może być wydalana z żółcią do przewodu pokarmowego. W badaniach z CS2 znakowanym izotopem siarki 35S, Strittmatter i współpr. (2) oznaczyli w kale od 5 do 15% zatrzymywanego przez organizm izotopu. CS2 podawany inhalacyjnie szczurom nie wykazuje jednak cech typowych dla związków hepatotoksycznych (3). Celem pracy była ocena przydatności modelu do badania związków siarki wydalanej z żółcią królików poddanych ekspozycji wysokich stężeń oparów dwusiarczku węgla, a także wstępna analiza kinetyki wydalania tych związków i ocena ich toksycznego działania na komórki wątrobowe.

9

Tolerance of Lactobacillus and Bifidobacterium strains to low pH, bile salts and digestive enzymes

67%

Olejnik A., Lewandowska M., Obarska M., Grajek W.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2005

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tom 08

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nr 1

Probiotic strains of L. casei, L. acidophilus, L. helveticus, B. animalis, B. lactis and two strains of B. bifidum were examined for their survival at pH 3.0 and 3% bile salts. Additionally, the viability of L. casei cells was tested in presence of pepsin, trypsin, chymotrypsin, alpha-amylase and lipase, and after heating at 90ºC for 30 min. It was found that all pre-treatments caused significant decrease in bacteria survival. The most sensitive strain for stress factors was B. bifidum 1. Using Caco-2 monolayer culture as the in vitro adhesion model, the significant reduction of adherence after thermal treatment, digestion with pepsin, trypsin and chymotrypsin, and bile salts were observed. The inhibition of bacteria adhesion after thermal denaturation and proteolysis proved an hypothesis that adhesion factors are of proteinaceous origin.

10

Surface and ES antigens of adult Dicrocoelium dendriticum inducing bile antibody responses in naturally infected cattle

67%

Wedrychowicz H., Ducommun D., Klockiewicz M., Pfister K.

Acta Parasitologica

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1996

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tom 41

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nr 3

Bile antibody responses of naturally infected cattle to surface and ES protein antigens of adult D. dendriticum were analysed. Freshly isolated and carefully purified flukes were surface labelled with NHS- biotin or biotin hydrazide (BH) and extracted using Tris-buffered saline. Biotinylated surface proteins or glycoproteins were then isolated by streptavidin affinity chromatography. ES products were obtained during 24 h incubation of undamaged worms at 37 °C in Tyrode’s salts solution enriched with antibiotics. Bile samples were collected at slaughter from cattle harbouring 120-280 lancet flukes. The fluke antigens inducing bile antibody responses were analysed by SDS-PAGE, Western blot and ELISA techniques. Bile from non-infected cattle precipitated none of the surface proteins and one polypeptide out of ES products. One to 6 polypeptides were found in immunocomplexes formed by individual bile samples of infected cattle and surface proteins while immunocomplexes formed by bile and surface extracts of flukes labelled with BH contained 2-5 fluke glycoproteins. An average of 3 polypeptides were recognised in ES products. In ELISA tests 38-67% out of 150 samples reacted with ES antigens while 70% possessed antibodies against surface proteins and 92% against surface glycoproteins. It is suggested that the surface and ES antigens may be of particular importance in the host-parasite relationship.

11

Bile flow, vesicular trafficking, and interactions of cytoskeleton with the canalicular domain of hepatocyte plasma membrane

67%

Pikula S., Bandorowicz-Pikula J.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 2

Liver is an epithelial organ which removes many substances from the blood, metabolizes them, and secretes back into circulation or directly into the bile. Liver parenchymal cells (hepatocytes) are involved in the overall detoxification of the organism through the bile. These highly polarized cells are unique among others due to the domain structure of their plasma membrane, organization of their cytoskeleton connected to the canalicular region of plasmalemma, and the specific distribution of various transport systems involved in detoxification phase III. In this mini-review the possible influence of canalicular motility modulated by cytoskeleton on the bile flow is discussed. In addition, the role of annexins, calcium- and phospholipid-binding proteins exhibiting high expression level in liver, in vesicular trafficking leading to the transport of some of biliary components is also postulated.

12

Local and systemic antibody response against surface, somatic and ES antigens of Dicrocoelium dendriticum in sheep naturally infected with the trematode

59%

Wedrychowicz H., Ducommun D., Bambara O. D., Pfister K.

Acta Parasitologica

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1997

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tom 42

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nr 4

Serum, bile and faecal protein extracts from slaughtered sheep harbouring lancet flukes, were used to investigate the range of Dicrocoelium dendriticum proteins provoking systemic and local antibody responses in naturally infected animals. ELISA and Western blot tests showed that the lancet fluke infection induced in sheep, serum and local antibody responses against Tris-buffered saline (TBS) soluble somatic proteins, surface proteins and glycoproteins as well as excretions and secretions (ES) of adult D. dendriticum. Sera of infected sheep contained antibodies against 10-14 somatic polypeptides (molecular mass range: 223-12.5 kDa), against 4 surface proteins and up to 9 surface glycoproteins. Antibodies against lancet fluke antigens were also found in 70% of bile samples and 41% of faecal samples. Approximately 40% out of serum samples analysed, contained antibodies against 10 somatic polypeptides and some 24% samples reacted with 12-14 antigens. Serum, bile and faecal samples from non-infected sheep did not recognise any of the lancet fluke proteins.

13

Stezenie olowiu,kadmu,miedzi i cynku w mozgowiu,plynie mozgowo-rdzeniowym,watrobie,zolci i krwi u bydla okolic Lublina

43%

Walkuska G., Saddour A.

Bromatologia i Chemia Toksykologiczna

|

1992

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tom 25

|

nr 4

349-354

Using AAS, Pb, Cd, Zn, and Cu concentrations were measured in the brain, cerebrospinal fluid, liver, bile, and blood in 10 weeks old calves, 10 month old young beef cattle, and 10-12 years old cows. The lowest concentration of Cd was observed in claves' brain, the higher - in the cerebrospinal fluid, blood and bile, and the highest - in the liver. Cadmium content in tissues and body fluids of cows and young beef cattle, was found to be several times higher when compared with that determined in calves. Contrary to Pb and Cd, the highest concentrations of Cu were noted in the cerebrospinal fluid, liver and bile of calves when compared with those determined in young beef cattle and cows. Similarly, the highest levels of Zn were found in the liver, brain, bile and cerebrospinal fluid of calves in comparison with those determined in young beef cattle and cows.

Ograniczanie wyników

Ultrasonographic examination of gallbladder contractility in the sheep

Gastroenterological research in domestic animals - past, present and future perspectives

The short history of gastroenterology

Evidence for probiotic potential of a capsular-producing Streptococcus thermophilus CHCC 3534 strain

Secretin, cholecystokinin and the biliary canalicular secretion in the rat

Survival of kefir bacteria in an acid environment and in the presence of bile

High performance liquid chromatography analysis of reduced glutathione in biological materials - report

Wydalanie siarki pojedynczej i podwojnej z zolcia krolikow zatruwanych dwusiarczkiem wegla

Tolerance of Lactobacillus and Bifidobacterium strains to low pH, bile salts and digestive enzymes

Surface and ES antigens of adult Dicrocoelium dendriticum inducing bile antibody responses in naturally infected cattle

Bile flow, vesicular trafficking, and interactions of cytoskeleton with the canalicular domain of hepatocyte plasma membrane

Local and systemic antibody response against surface, somatic and ES antigens of Dicrocoelium dendriticum in sheep naturally infected with the trematode

Stezenie olowiu,kadmu,miedzi i cynku w mozgowiu,plynie mozgowo-rdzeniowym,watrobie,zolci i krwi u bydla okolic Lublina