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  1. 4 Acta Biologica Cracoviensia. Series Zoologia

  2. 3 Acta Physiologiae Plantarum

  3. 2 Acta Biochimica Polonica

  4. 2 Folia Histochemica et Cytobiologica

  5. 2 Journal of Applied Genetics

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  1. 3 Knas M.

  2. 3 Kolataj A.

  3. 3 Krol T.

  4. 3 Zwierz K.

  5. 2 Augustyniak J.

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  1. 1 2017

  2. 2 2013

  3. 1 2012

  4. 1 2011

  5. 2 2010

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1

Beta-glucuronidase and beta-glucosidase activity in stool specimens of children with inflammatory bowel disease

100%
Mroczynska M., Galecka M., Szachta P., Kamoda D., Libudzisz Z., Roszak D.
Polish Journal of Microbiology
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2013
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tom 62
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nr 3
The aim of the study was to analyze the differences in the activity of β-glucuronidase and β-glucosidase in stool specimens of children with Inflammatory Bowel Diseases (IBD) and healthy subjects. The disease activity was determined according to the PCDAI scale (Crohn disease) and Truelove-Witts scale (Ulcerative colitis). Enzyme activity was determined by spectrophotometry. There was a correlation between the level of β - glucosidase activity in stool and patient’s age in the group of healthy controls, but not in the IBD group. β-glucosidase activity in IBD and healthy subjects stool specimens did not differ significantly. The activity of β-glucuronidase in children with IBD was two times lower than in the healthy group and was correlated with age in children with IBD, but not in the group of healthy ones.
2

Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

100%
Pluskota W. E., Michalczyk D. J., Gorecki R. J.
Acta Physiologiae Plantarum
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2005
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tom 27
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nr 2
The gene fusion system was used to study UV light-control of PS PAL1 and PS PAL2 genes encoding phenylalanine ammonia-lyase of pea. The induction of pea PAL promoters was analysed in transgenic tobacco plants. Binary plasmids (derivatives of pBI101.2 vector) containing 5’ regulatory fragments of PS pAl1 and PS PAL2 linked to reporter genes (GUS, LUC) were constructed. The analyses were performed with the use of single constructs (containing one variant of PS PAL promoter and one reporter gene) and dual constructs (containing both PS PAL1 and PS PAL2 promoters connected with different reporter genes). The use of dual constructs enabled the evaluation of both PS PAL promoters activity in the same plant. The analyses of in vitro grown plants have shown that both PAL promoters are strongly induced in leaves subjected to UV radiation. In some cases, the UV-stimulated expression exceeded the exposed areas. This phenomenon was observed more often in the leaves of plants containing the PS PAL1:: GUS than PS PAL2::GUS construct. Removal ofboxes 2, 4, 5 from PS PAL1 promoter and deletion of its 5’end region (-339 to -1394) decreases the level of gene expression but does not eliminate its responsiveness to UV.
3

Optimisation of particle bombardment parameters for shoot primordia explants of Cucumis sativus L.

100%
Czarnak M., Burza W., Filipecki M., Rakoczy-Trojanowska M.
Journal of Applied Genetics
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1996
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tom 37A
4

Increased granulocyte heparanase activity in neutrophils from patients with lupus nephritis and idiopathic membranous nephropathy

84%
Szymczak M., Kuzniar J., Kopec W., Zabinska M., Marchewka Z., Koscielska-Kasprzak K., Klinger M.
Archivum Immunologiae et Therapiae Experimentalis
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2017
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tom 65
|
nr 1
5

The profile of lysosomal exoglycosidases in replicative and stress-induced senescence in early passage human fibroblasts

84%
Knas M., Zalewska A., Kretowski R., Niczyporuk M., Waszkiewicz N., Cechowska-Pasko M., Waszkiel D., Zwierz K.
Folia Histochemica et Cytobiologica
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2012
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tom 50
|
nr 2
6

Beta-glucuronidase and beta-glucosidase activity of Lactobacillus and Enterococcus isolated from human feces

84%
Mroczynska M., Libudzisz Z.
Polish Journal of Microbiology
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2010
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tom 59
|
nr 4
The domination of microorganisms characterized by excessive activity of the so-called fecal enzymes may be one of the reasons of the large intestine cancers. These enzymes are mainly those that belong to the hydrolase and reductase classes and their excessive activity may lead to disorders in the functioning of the digestive tract. The aim of tise research was to determine the activity of β-glucuronidase and β-glucosidase of Lactobacillus and Enterococcus strains isolated from the feces of healthy children, aged 1 and 8, and adults, aged 30 and.80. The analysis included 10 strains isolated from the feces of individuals in each of the age groups. β-glucuronidase activity in the case of the isolates from children, depending on the strain, equaled from about 0.15 mM/h/mg of protein to 0.26 mM/h/mg of protein and was lower, respectively, by 52.35% and 57.81%, than the β-glucosidase activity. Simultaneously, the activity of the Lactobacillus enzymes from children was 2.4 times higher, and in case of the isolates obtained from adults they were 4.6 and 2.7 times higher than the activity of the Enterococcus enzymes. The highest β-glucuronidase activity was observed in Lactobacillus isolates coming from an 80-year-old subject. The differences between the activity of Enterococcus β-glucuronidase isolated from the feces of 1 and 8 year old children were statistically insignificant. On the other hand, in the case of the subjects aged 30 and 8 the isolates were characterized by activity lower by, respectively. 48% and 37% than the isolates coming from children. The highest β-glucosidase activity was discovered in the case of Lactobacillus and Enterococcus coming from children, which was higher by 32% than the activity of the isolates from adult persons. Therefore, it was determined that the activity of β-glucuronidase of Lactobacillus strains isolated from feces from people aged 80 was the highest, and the isolates of the examined microorganisms coming from children were characterized by the highest β-glucosidase activity.
7
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Activity of alpha-fucosidase and beta-glucuronidase in serum and urine of patients administered parenteral nutrition

84%
Raczkowska K., Szajda S. D., Raczkowski K., Zasadowska W., Chojnowska S., Kepka A., Zalewska-Szajda B., Waszkiewicz N., Knas M., Snarska J., Zwierz K., Ladny J. R.
Roczniki Państwowego Zakładu Higieny
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2013
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tom 64
|
nr 3
Background. In hospital patients suffering from adverse clinical and biochemical symptoms of malnutrition, it is often necessary to employ parenteral nutrition to avoid the body’s tissue becoming broken down by being metabolised. Thus, the patient’s welfare and survival can be supported throughout any periods of medical crisis. Two of the enzymes responsible for metabolising glycoconjugates are a-fucosidase (FUC) and p-glucuronidase (GLU), present in lysosomes. They release fucose or glucuronic acid from the non-reducing end of oligosaccharide chains. Objective. To determine the effect of parenteral nutrition administered to ill patients, on glycoconjugate metabolism, by measuring serum and urinary activities of FUC and GLU. Material and methods. Blood samples and the daily urine collection were taken from 23 patients’ who had been undergoing parenteral nutrition for either 5 or 10 days, as well as from a baseline sample. Enzyme activities in serum and urine were determined by the method of Zwierz et al. Results. Serum FUC activities were significantly lower after 10 days compared to 5, (p< 0.0172), whereas GLU activities were significantly lower after both 5 and 10 days, (p< 0.0007 and p< 0.0208 respectively), compared to levels before starting parenteral nutrition. GLU activities were however higher after 10 days than those after 5 days, (p< 0.0023). In urine, FUC activities were significantly decreased after 10 days compared to 5 days after starting parenteral nutrition, (p< 0.0245). Urine GLU activities were unaffected by parenteral nutrition nor was any effect seen on FUC or GLU activities when calculated per 1mg creatinine. Conclusions. Serum FUC and GLU activities can be used for assessing the effect of parenteral nutrition on glycoconjugate metabolism. The significant decreases of serum GLU activity observed after 5 and 10 days, may serve to indicate that the components of parental nutrition are appropriate and that the body has become suitably adapted to this form of nutrition.
8

Application of tRNA genes as promoters for protein coding genes in plants

84%
Kaczmarek W. B., Augustyniak J.
Journal of Applied Genetics
|
1996
|
tom 37A
9

35S-CaMV driven GUS activity during the culture of microspores from a transgenic Brassica napus L. plant

84%
Klein M., Custers J., Van Lookeren Campagne M.
Acta Physiologiae Plantarum
|
1997
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tom 19
|
nr 2
10

The influence of a high intake of exogenous antioxidants on the activity of beta-glucuronidase [B-GL] in the mouse liver kidney and blood plasma

84%
Sliwa-Jozwik A., Jozwik A., Fronczyk W., Kolataj A.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
11

Variation of beta-glucuronidase activity in cauliflower plants with gus gene introduced by Agrobacterium rhizogenes mediated transformation

84%
Baranski R., Puddephat I.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 1
12

Effect of nuclear matrix attachment regions on transgene expression in tobacco plants

84%
Nowak W., Gawlowska M., Jarmolowski A., Augustyniak J.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 3
Matrix attachment regions (MARs) are thought to participate in the organization and segregation of independent chromosomal loop domains. Although there are sev­eral reports on the action of natural MARs in the context of heterologous genes in transgenic plants, in our study we tested a synthetic MAR (sMAR) with the special property of unpairing when under superhelical strain, for its effect on reporter gene expression in tobacco plants. The synthetic MAR was a multimer of a short sequence from the MAR 3 end of the immunoglobulin heavy chain (IgH) enhancer. This sMAR sequence was used to flank the β-glucuronidase (GUS) reporter gene within the T-DNA of the binary vector pBI121. Vectors with or without the sMARs were then used to transform tobacco plants by Agrobacterium tumefaciens. Transgenic plants containing the sMAR sequences flanking the GUS gene exhibited higher levels of transgene expression compared with transgenic plants which lacked the sMARs. This effect was observed independently of the position of the sMAR at the 5 side of the re­porter gene. However, variation of the detected transgene expression was significant in all transformed plant populations, irrespective of the construct used.
13

The effects of dehydroepiandrosterone on the activity of selected lysosomal enzymes in mice from different age groups

67%
Krol T., Lysek-Gladysinska M., Lach H., Kochman K., Trybus W., Staszczyk K., Mycinska M., Kopacz-Bednarska A., Trybus E., Wieczorek A., Gren A.
Acta Biologica Cracoviensia. Series Zoologia
|
2008
|
tom 50
37-42
14

A dose-response effects of tannic acid and protein on growth performance, caecal fermentation, colon morphology, and beta-glucuronidase activity of rats

67%
Barszcz M., Taciak M., Skomial J.
Journal of Animal and Feed Sciences
|
2011
|
tom 20
|
nr 4
15

Engineered resistance against proteinases

67%
Milner M., Chroboczek J., Zagorski-Ostoja W.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 3
Exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. We examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. Two proteinase inhibitors were studied: a Kazal-type silk proteinase inhibitor (SPI2) from Galleria mellonella, and the Cucurbita maxima trypsin inhibitor I (CMTI I). Both inhibitors target serine proteinases, are small proteins with a compact structure stabilized by a network of disulfide bridges, and are expressed as free polypeptides in their natural surroundings. Four constructs were prepared: the gene for either of the inhibitors was ligated to the 5' end of the DNA encoding one or the other of two selected target proteins, the coat protein (CP) of Potato potyvirus Y or the Escherichia coli β-glucuronidase (GUS). CMTI I fused to the target proteins strongly hampered their functions. Moreover, the inhibitory activity of CMTI I was retained only when it was fused to the CP. In contrast, when fused to SPI2, specific features and functions of both target proteins were retained and the inhibitory activity of SPI2 was fully preserved. Measuring proteolysis in the presence or absence of either inhibitor, we demonstrated that proteinase inhibitors can protect target proteins used either free or as a fusion domain. Interestingly, their inhibitory efficiency was superior to that of a commercial inhibitor of serine proteinases, AEBSF.
16

Biochemical and ultrastructural changes in mouse hepatocytes after encumbrance with Iscador

67%
Krol T., Wieczorek A., Lysek-Gladysinska M., Witek B., Lach H., Pierzchala-Koziec K., Gren A.
Acta Biologica Cracoviensia. Series Zoologia
|
2003
|
tom 45
17

Activity of the lysosomal system in mouse liver after hydrocortisone administration

67%
Krol T., Kolataj A.
Acta Biologica Cracoviensia. Series Zoologia
|
2000
|
tom 42
18

Effect of glutathione on the activity of lysosomal enzymes in the liver and kidney on mice

67%
Witek B., Kolataj A.
Acta Biologica Cracoviensia. Series Zoologia
|
2000
|
tom 42
19

The activity of selected glycosidases in salivary gland tumors

59%
Bierc M., Minarowski L., Wozniak L., Chojnowska S., Knas M., Szajda S., Zwierz K.
Folia Histochemica et Cytobiologica
|
2010
|
tom 48
|
nr 3
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