Wyniki wyszukiwania

1

Screening of microorganisms for improvement of beta-galactosidase production

100%

Fiedurek J., Ilczuk Z.

Acta Microbiologica Polonica

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1990

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tom 39

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nr 1-2

2

Fragment C tetanus toxin: A putative activity-dependent neuroanatomical tracer

86%

Miana-Mena F. J., Munoz M. J., Ciriza J., Soria J., Brulet P., Zaragoza P., Osta R.

Acta Neurobiologiae Experimentalis

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2003

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tom 63

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nr 3

3

A lysosomal high molecular weight multienzyme complex

86%

Ostrowska H., Jedynasta-Kozlowska W., Kalinowska J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors

86%

Tabbaa S., Goulah C., Tran R. K., Lis A., Korody R., Stachowski B., Horowitz J. M., Torres G., Stachowiak E. K., Bloom D. C., Stachowiak M. K.

Folia Morphologica

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2000

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tom 59

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nr 4

Manipulation of gene expression in developing or in mature central nervous systems (CNS) holds a promise for the resolution of many compelling neurobiological questions, including the feasibility of gene therapy to treat diseases of the brain. In this context, a number of viral vectors have been used in recent years to introduce and express genes into the CNS. This article discusses a gene transfer system based on the Herpes Simplex Virus-1 (HSV-1). We describe here the use of non-replicating, non-toxic HSV-1 vector, 8117/43, in a series of studies carried in our joint program. This vector proves further the utility of HSV-1 as a delivery vehicle to a number of distinct sites within the CNS.

5

An attempt to explain the reason of missing lactose fermentation capability in Yersinia pseudotuberculosis

86%

Skwark M.

Advances in Agricultural Sciences

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2002

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tom 08

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nr 1

6

Testicular activity of acid glycosidases in cocks of different breeds

86%

Droba B.

Roczniki Naukowe Zootechniki

|

2000

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tom 27

|

nr 4

7

The profile of lysosomal exoglycosidases in replicative and stress-induced senescence in early passage human fibroblasts

72%

Knas M., Zalewska A., Kretowski R., Niczyporuk M., Waszkiewicz N., Cechowska-Pasko M., Waszkiel D., Zwierz K.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 2

8

Utilization of UF-permeate for production of beta-galactosidase by lactic acid bacteria

72%

Murad H. A., Refaea R. I., Aly E. M.

Polish Journal of Microbiology

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2011

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tom 60

|

nr 2

Four lactobacilli strains (Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacilus casei and Lactobacillus reuteri) were grown in MRS broth and three lactococci strains (Streptococcus thermophilus, Lactococcus lactis subsp. Lactis and Lactococcus lactis subsp. lactis biovar. diacetilactis) were grown in M17 broth. L. reuteri and S. thermophilus were chosen on the basis of the best mean β-galactosidase activity of 10.44 and 10.01 U/ml respectively, for further studies on permeate-based medium. The maximum production of β-galactosidase by L. reuteri was achieved at lactose concentration of 6%, initial pH 5.0–7.5, ammonium phosphate as nitrogen source at a concentration of 0.66 g N/L and incubation temperature at 30°C/24 hrs to give 6.31 U/ml. While in case of S. thermophilus, maximum β-galactosidase production was achieved at 10% lactose concentration of permeate medium, supplemented with phosphate buffer ratio of 0.5:0.5 (KH2PO4:K2HPO4, g/L), at initial pH 6.0–6.5, ammonium phosphate (0.66g N/L ) as nitrogen source and incubation temperature 35°C for 24 hrs to give 7.85 U/ml.

9

Expression of microinjected reporter gene lacZ during first cleavages of rabbit embryos

72%

Rosochacki S. J., Strzelecka M., Kozikova L., Matejczyk M., Oblap R., Poloszynowicz J., Zwierzchowski L.

Folia Biologica

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2002

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tom 50

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nr 1-2

10

The application of immobilized biocatalyzers to intensity the ethanol fermentation of lactose

72%

Lewandowska M., Bednarski W., Adamczak M., Tomasik J., Narwojsz D.

Polish Journal of Food and Nutrition Sciences

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2002

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tom 11/52

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nr 4

Ethanol fermentation of lactose mash by biocatalyzers immobilized in calcium alginate was studied in order to improve the process productivity and economy. The fermentation effectiveness of S. cerevisiae co-immobilized with a ß-galactosidase and the directly lactose-fermenting immobilized yeast: K. fragilis and C. pseudotropicalis were compared. The application of the immobilized K. fragilis produced desirable results and even after its 10th (20 days) fermentation the immobilized yeast provided the stable high fermentation level (on average about 6%/vol of ethanol) while maintaining its activity. Such lactose-mash fermentation was greater than in conventional method (by free cells). In addition, the application of S. cerevisiae co-immobilized with ß-galactosidase produced somewhat greater fermentation levels than the conventional method.

11

Glycosidases and acid-invertase activities are not correlated with Phaseolus hypocotyl growth

72%

Bagatharia S. B., Chanda S. V.

Acta Physiologiae Plantarum

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1998

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tom 20

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nr 4

Changes in α-galactosidase, β-galactosidase, β-glucosidase and acid invertase activities were examined in Phaseolus vulgaris hypocotyls treated with gibberellic acid (GA), naphthyl acetic acid (NAA) and distilled water (DW) (control) in light condition. The activities were estimated both in cytoplasmic and ionically wall-bound fraction. The upper segment showed considerable elongation growth while there was hardly any growth in lower segment. GA and NAA showed distinct promotion and inhibition respectively in hypocotyl growth in upper segment. The glycosidase activities were detected in both the fractions but the activity was more pronounced in cytoplasmic than in wall fraction. Acid invertase activity was present only in cytoplasmic fraction. In lower segment, in both cytoplasmic and wall fraction, the glycosidase activity, in general, showed a decreasing trend and no effect of treatment could be envisaged. In upper segment, though the trend was similar to the lower one, in α- and β-galactosidase NAA treated segment had more activity. Invertase activity also did not show a clear trend to implicate its function in hypocotyl elongation growth. The results are discussed in relation to establishing a correlation between an activity (glycosidase and invertase) and a physiological process (hypocotyl elongation). It is concluded that these wall-loosening enzymes have no role in elongation growth of Phaseolus vulgaris hypocotyls.

12

The attemps to intensity synthesis of galactooligosaccharides in the process of enzymatic lactose hydrolysis - short report

72%

Kowalewska-Piontas J., Bednarski W.

Polish Journal of Food and Nutrition Sciences

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2001

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tom 10

|

nr 3

Transgalactosylation properties of the commercial preparations of beta-galactosidase: Lactase and Maxilact and of the preparation synthesized by Penicillium canescens, were compared.The effect of 2% glucose addition to 20% milk permeate solution on the process of formation of galac- tooligosaccharides during the enzymatic bioconversion of lactose by the examined beta-galactosidase preparations, was also studied. It was found that enzymatic synthesis of galactooligosaccharides in the process of lactose bioconversion was greatly dependent on beta-galactosidase used and that the presence of glucose in lactose solution already at the beginning of hydrolysis was favourable for the formation of oligosaccharides.

13

Effect of colostrinin, an immunomodulatory proline-rich polypeptide from ovine colostrum, on sialidase and beta-galactosidase activities in murine thymocytes

72%

Sokal I., Janusz M., Miecznikowska H., Kupryszewski G., Lisowski J.

Archivum Immunologiae et Therapiae Experimentalis

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1998

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tom 46

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nr 3

14

Presentation of beta-galactosidase activity secreted by plant cells

72%

Stano J., Micieta K., Grancai D., Blanarikova V.

Herba Polonica

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2007

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tom 53

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nr 1

Extracellular plant β-galactosidase from opium poppy and celandine was detected on agar plates by the presence of dyed zones from 1-naphthyl-β-D-galactopyranoside used as a synthetic substrate. Evaluating the azo-dye zones assessed the degree β-galactosidase activity. No coloration of the agar medium was observed on non-inoculated parts, in medium inoculated with heat – inactivated cells (10 min. at 100oC) or in medium without substrate with all calli tested. On the agar plates with substrate and sterile opium poppy, celandine or gherkin seedlings (2–6 days old) changes in coloration were observed showing the release of β-galactosidase from the roots during germination.

15

Lysosomal high molecular weight multienzyme complex

72%

Ostrowska H., Krukowska K., Kalinowska J., Orlowska M., Lengiewicz I.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 1

Three acidic glycosidases: β-galactosidase (β-GAL, EC 3.2.1.23), α-neuraminidase (NEUR, sialidase, EC 3.2.1.18), N-acetylaminogalacto-6-sulfate sulfatase (GALNS, EC 3.1.6.4) and serine carboxypepidase cathepsin A (EC 3.4.16.1) form a functional high molecular weight complex in the lysosomes. The major constituent of this complex is cathepsin A, the so-called “lysosomal protective protein” (PPCA). By forming a multienzyme complex, it protects the glycosidases from rapid intralysosomal proteolysis, and it is also required for the intracellular sorting and proteolytic processing of their precursors. In man, a deficiency of cathepsin A leads to a combined deficiency of β-GAL and NEUR activities, called “galactosialidosis”. Multiple mutations identified in the cathepsin A gene are the molecular basis of this lysosomal storage disease. This review describes the structural organization of the lysosomal high molecular weight multienzyme complex and the importance of the protective protein/cathepsin A in physiology and pathology.

16

Assessment of viral and non-viral gene transfer into adult rat brains using HSV-1, calcium phosphate and PEI-based methods

58%

Corso T. D., Torres G., Goulah C., Roy I., Gambino A. S., Nayda J., Buckley T., Stachowiak E. K., Bergey E. J., Pudavar H., Dutta P., Bloom D. C., Bowers W. J., Stachowiak M. K.

Folia Morphologica

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2005

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tom 64

|

nr 3

130-144

CNS gene transfer could provide new approaches to the modelling of neurodegenerative diseases and devising potential therapies. One such disorder is Parkinson’s disease (PD), in which dysfunction of several different metabolic processes has been implicated. Here we review the literature on gene transfer systems based on herpes simplex virus type 1 (HSV-1) and non-viral polyethyleneimine (PEI) and calcium phosphate nanoparticle methods. We also assess the usefulness of various CNS gene delivery methods and present some of our own data to exemplify such usefulness. Our data result from vectors stereotaxically introduced to the substantia nigra (SN) of adult rats and evaluated 1 week and/or 1 month post injection using histochemical methods to assess recombinant β-galactosidase enzyme activity. Gene transfer using PEI or calcium phosphate-mediated transfections was observed for both methods and PEI was comparable to that of HSV-1 amplicon. Our data show that the amplicon delivery was markedly increased when packaged with a helper virus and was similar to the expression profile achieved with a full-size replication-defective HSV-1 recombinant (8117/43). We also examine whether PEI or HSV-1 amplicon-mediated gene transfer could facilitate assessment of the biological effects induced by a dominant negative FGF receptor-1 mutant to model the reduced FGF signalling thought to occur in Parkinson’s disease.

17

Low-glucose medium induces ORP150 expression and exerts inhibitory effect on apoptosis and senescence of human breast MCF7 cells

58%

Kretowski R., Stypulkowska A., Cechowska-Pasko M.

Acta Biochimica Polonica

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2013

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tom 60

|

nr 2

Glucose deprivation is a factor evoking endoplasmic reticulum (ER) stress and induction of expression of an oxygen-regulated protein of 150 kDa (ORP150). We studied the effect of inducible overexpression of ORP150 on senescence and apoptosis of human breast carcinoma cells (MCF7) and human skin fibroblasts. We found an inhibitory effect of ORP150 on apoptosis and senescence of MCF7 cells, but not fibroblasts in ER stress conditions. An increased expression of senescence-associated β-galactosidase and acid β-galactosidase activity (biomarkers of cellular senescence) was observed. We suggest that ORP150 induction in cancer cells can promote tumour progression and may be a major cause of their resistance to chemotherapeutics.

18

Beta-galactosidase [beta-gal] from the yeast Rhodotorula ingeniosa and its utilization in ice milk production

58%

El-Sayed E., El-Hofi M.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2010

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tom 13

|

nr 1

19

Expression of the transcription factor regulatory factor X1 in the mouse brain

58%

Feng C., Li J., Zuo Z.

Folia Histochemica et Cytobiologica

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2011

|

tom 49

|

nr 2

20

Alpha fucosidase and beta galactosidase in serum of a Lyme disease patients as a possible marker of accelerated senescence — a preliminary study

58%

Wasiluk A., Waszkiewicz N., Szajda S. D., Wojewodzka-Zelazniakowicz M., Kepka A., Minarowska A., Zwierz Z. W., Pancewicz S., Zalewska-Szajda B., Ladny J. R., Zwierz K.

Folia Histochemica et Cytobiologica

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2012

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tom 50

|

nr 2

Ograniczanie wyników

Screening of microorganisms for improvement of beta-galactosidase production

Fragment C tetanus toxin: A putative activity-dependent neuroanatomical tracer

A lysosomal high molecular weight multienzyme complex

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors

An attempt to explain the reason of missing lactose fermentation capability in Yersinia pseudotuberculosis

Testicular activity of acid glycosidases in cocks of different breeds

The profile of lysosomal exoglycosidases in replicative and stress-induced senescence in early passage human fibroblasts

Utilization of UF-permeate for production of beta-galactosidase by lactic acid bacteria

Expression of microinjected reporter gene lacZ during first cleavages of rabbit embryos

The application of immobilized biocatalyzers to intensity the ethanol fermentation of lactose

Glycosidases and acid-invertase activities are not correlated with Phaseolus hypocotyl growth