Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: beta-1,3-1,6-D-glucan

Sortuj według:

Ogranicz wyniki do:

Effect of beta-1,3-1,6-D-glucan on the phagocytic activity and oxidative metabolism of pripheral blood granulocytes and monocytes in rats

100%

Wojcik R., Janowska E., Malaczewska J., Siwicki A. K.

Bulletin of the Veterinary Institute in Puławy

2009

tom 53

nr 2

241-246

The experiment was performed on 20 adult Wistar rats aged 12 weeks, divided into two equal groups (control and experimental), each comprised of five males and five females. From the first day of the experiment, the experimental group rats were fed Murigran feed supplemented with ß-l,3/l,6-D-glucan at a dosage of 12-19 mg/rat/d, subject to body weight, while the control- group was administered the same feed without any additives. At the beginning of the experiment and then after 14 days, arterial blood samples were collected from the rats and diluted with heparin to measure and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. Statistically higher levels of the activity were observed in the group of rats administered glucan than in controls, expressed in terms of the percentage of phagocytic cells as well as average fluorescence intensity. ß-l,3/l,6-D-glucan also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with E. coli, and on the oxidative metabolism of granulocytes after stimulation with PMA.

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide

67%

Wojcik R.

Polish Journal of Veterinary Sciences

2010

tom 13

nr 1

181-188

The objective of this study was to determine the effect of ß-l,3/l,6-D-glucan (Biolex-Beta HP) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. The experimental material comprised 40 adult Wistar rats aged 14 weeks, divided into two equal groups, a control group and an experimental group, each of 20 adult rats, including 10 males and 10 females. In the course of 3 successive days, 20 rats from the experimental group were administered cyclophosphamide intramuscularly at a dose of 50 mg/kg body weight/day. On the 8th day of the experiment, 10 control group (K) rats and 10 experimental group (C) rats were sacrificed. Arterial blood samples were collected and diluted with heparin to determine and compare the phagocytic activity (Phatogest) and oxidative metabolism (Bursttest) of peripheral blood granulocytes and monocytes by flow cytometry. Starting on the 8lh day of the experiment, the feed of the remaining 10 rats from the experimental group (C+G) and 10 rats from the control group (G) was supplemented with Biolex-Beta HP at a dose of 50 mg/kg body weight/day for 14 consecutive days. On day 22, arterial blood samples were collected from all (C+G) and (C) group rats, diluted with heparin to determine and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. The results showed that Biolex-Beta HP modulated the phagocytic activity and oxidative metabolism of blood neutrophils and monocytes suppressed by cyclophosphamide in rats. The immunorestoring activity of Biolex-Beta HP was observed in this study.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Polish Journal of Veterinary Sciences

2 Wojcik R.

1 Janowska E.

1 Malaczewska J.

1 Siwicki A. K.

1 2010

1 2009

Wyniki wyszukiwania

Effect of beta-1,3-1,6-D-glucan on the phagocytic activity and oxidative metabolism of pripheral blood granulocytes and monocytes in rats

Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide