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Twenty six European brown bears Ursus arctos Linnaeus, 1758 were captured, radio-collared, and tracked in and around Plitvice Lakes and Risnjak National Parks from 01 November 1981 to 25 July 1991. The largest male and female ranges were 224 km2 and 147 km2, respectively. Mean annual ranges were 128 km2 and 58 km^ for males and females, respectively. Male ranges were 2 to 5 times larger than female ranges. The median straight-line distance between consecutive day locations was 1.5 km for a!! bears and the maximum distance was 8.5 km. Distances traveled daily by males and females were similar, but movements of females were restricted to smaller areas, Winter ranges were significantly smaller than ranges during other seasons. Marked bears spent 52% of their time outside the Plitvice Lakes and Risnjak National Parks.
The sites of 87 brown bear Ursus arctos Linnaeus, 1758 attacks on livestock (horses, cattle, sheep, goats) were investigated in the Cantabrian Mountains, Spain. Ninety percent of livestock predation occurred between May and October, while attacks were most frequent during the months of May and July. There was no difference in overall attack rates between the first and second part of the year, nor among livestock type or age-class. Bears were not selective predators of livestock type or age-class, but had a slight tendency to attack more cattle and adult animals. Bear tracks and scats were found at slightly more than half of the sites visited, while bed construction and food-caching behaviour was rarely detected. Attacks were initiated most times on the neck and the head region. Muscle tissue and soft organs were most preferred body parts consumed by brown bears.
Bieszczady Mountains of Poland. Acta theriol. 37: 339 - 344. The diet of brown bear Ursus arctos Linnaeus, 1758 in the autumn of 1990 and the spring of 1991 was studied by the analysis of faeces contents (46 samples, 23 from autumn and 23 from spring). Based on frequency of occurrence of food items in individual faeces, the autumn diet of bears was more diverse than the spring diet. We calculated for each food item frequency percent (F%), dry weight percent (W%) and Importance Value (IV%). Beech Fagus silvatica nuts were the most important food during both spring and autumn (36.9% IV in autumn and 78.5% IV in spring). Carrion, used as a bait by hunters, and foodstuffs from game-feeding stations (maize, oats, beets) also appeared to be a significant part of the bears' diet.
Ancient DNA from bones of the extinct Ursus spelaeus Rosamueller et Hainroth, 1794 found in the Bavarian Alps has been amplified by PCR. Two out of five samples yielded a distinct band of 135 bp originating from the mtDNA control region. A combination of nested and touchdown PCR supported the amplification. Analysis of the nucleotide sequences revealed four transitions compared to the French cave bear sequence, the only cave bear data known so far (Genbank database X80259, AF121779). The consensus distant matrix tree clustered the two cave bears next to the brown bear Ursus arctos Linnaeus, 1758.
During the last centuries many West European populations of wolf Canis lupus Linnaeus, 1758 and brown bear Ursus arctos Linnaeus, 1758 have been extirpated from most of their former ranges. Isolated populations of wolves (about 300 - 400 animals) and brown bears (about 80 - 100 animals) actually survive in the Italian Apennines, while very few (5 - 10) brown bears remain in the Italian eastern Alps. We have investigated the consequences of isolation, demographic decline, and random drift on genetic variability of the Italian populations of wolf and brown bear using restriction site analysis and nucleotide sequencing of portions of the mitochondrial genome. The studied sequences were homogeneous within-populations of both species, but there was a fixed difference in mtDNA between brown bears form the Alps and from the Apennines. Random drift since the time of isolation is a plausible explanation for both results. These findings suggest that wolves and bears have small effective population sizes and, thus, they will continue to loose genetic variability by random drift in the near future. Conservation efforts should be directed towards an increase of the annual growth rates of these populations. The individualization of discrete phylogeographic units in the brown bear suggests to manage them separately in order to preserve the existing gene diversity among populations.
This paper is focused on cloning and bioinformatical as well as immunological characterisation of the new vaccine antigen candidate against fasciolosis - Fasciola hepatica phosphoglycerate kinase (FhPGK). The antigen was cloned from the adult fluke by the use of RACE-PCR method. It was found that FhPGK is not a secretory and not a stage specific protein. It is present in all kinds of parasite tissue, particularly in fluke intestine and tegumental as well as subtegumental layers. FhPGK is involved in production of the first ATP molecule in the glycolytic pathway and can be used in vaccination trials in which the strategy is to block fluke's energy metabolism. This is the first, to date, phosphoglycerate kinase cloned from F. hepatica.
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