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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Ultrastructural changes in leaf cells infected with Arabis mosaic nepovirus. I. In bean plants [Phaseolus vulgaris L.]

100%
Zielinska L., Pospieszny H.
Journal of Plant Protection Research
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2001
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tom 41
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nr 2
Two experimental methods were used in the study. The aim of the first one was focused on a detection of Arabis mosaic nepovirus (ArMV) particles and tubules with viruses in extracts obtained from crushed leaves of bean. Second one consisted on investigation of ultrastructural changes occuring in the bean leaf tissues with symptoms caused by ArMV. Characteristic membranous inclusions in the cytoplasm were observed and described. ArMV occured either as irregularly scattered particles in the cytoplasm, crystal-like aggregates or semiconcentric and concentric layers. Not numerous but sometimes very long tubules with viruses were mainly observed near the cell wall. Protrusion of the cell wall into the protoplast very often containing viruses in the plasmodesmata were observed many times. Plasmalemmasomes were frequently situated near the cell wall.
2
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Partial characterization of Sunn-hemp mosaic tobamovirus [SHMV] isolated from bean plants [Phaseolus vulgaris L.]

100%
Pospieszny H., Palczewska M., Borodynko N.
Journal of Plant Protection Research
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2001
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tom 41
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nr 2
This work presents some properties of Sunn-hemp mosaic tobamovirus (SHMV) orginally isolated from bean plants. Virus infected host range and induced symptoms that were typical for SHMV. Following plant species distinquished SHMV from tobacco mosaic tobamovirus (TMV): Phaseolus vulgaris, Pisum sativum, Lupinus albus and Lycopersicon esculentum. In immunoblotting the serum against SHMV did not react with TMV and Tomato mosaic tobamovirus (ToMV). The electrophoretical patterns of whole virions and capsid proteins were characteristic for SHMV and different from that of TMV and ToMV.
3

Different effect of Ca concentration on Cu2 plus - induced changes in bean plants

100%
Maksymiec W., Baszynski T.
Biological Bulletin of Poznań. Supplement
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1997
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tom 34
4

Relationship between peroxidase activity and the amount of fully N-methylated compounds in bean plants infected by Pseudomonas savastanoi pv. phaseolicola

100%
Sardi E., Stefanovits-Banyai E.
Acta Physiologiae Plantarum
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2006
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tom 28
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nr 2
Changes in the level of endogenous formaldehyde (HCHO), some N-methylated compounds (choline and trigonelline) and peroxidase activity were examined in the leaves of bean genotypes (Phaseolus vulgaris L.) with different disease-sensitivity during ontogenesis in the stressfree condition and after natural infection by Pseudomonas savastanoi pv. phaseolicola (until the appearance of lesions). HCHO, as its dimedone adduct, and fully N-methylated compounds were determined by overpressured layer chromatography (OPLC) in different developmental stages and in the infected leaves/leaf discs. Peroxidase activity was measured by a spectrophotometric method. HCHO level decreased with agetng of the primary leaf and accordingly in the leaves at different developmental stages, then increased again in both cases due to the demethylation and methylation processes. Concentration of choline and trigonelline as potential HCHO generators des creased considerably while peroxidase activity increased with agetng of the plants. Comparing the symptomless and the Pseudomonas infected leaf discs (with watersoaked lesions) we found a decrease in the level of HCHO, choline and trigonelline and there was det ectable increase in the peroxidase activity in the infected leaftissues. Our findings are in accordance with previously published results that peroxidases play an important role in oxidative demethylation processes. Our hypothesis is that the high level of HCHO in the old leaves can originate from methylated components as the result of peroxidase activity and this high level may lead to the old leaf betng resistant to pathogen. This conclusion is supported by the fact that the leaves of susceptible bean genotypes became resistant to Pseudomonas while growing older.
5
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Effect of soil salinity on activity of antioxidant enzymes and content of ascorbic acid and phenols in bean [Phaseolus vulgaris L.] plants

80%
Telesinski A., Nowak J., Smolik B., Dubowska A., Skrzypiec N.
Journal of Elementology
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2008
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tom 13
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nr 3
Soil salinity is the one of the most important abiotic factors influencing the growth, development and yields of crops. However, it is difficult to determine exact concentrations of salt which cause soil salinity. Salinity threshold levels depend on a crop species, variety, developmental stage and environmental factors. This paper presents the results of an experiment on the effect of different soil concentrations of NaCl soil on several oxidation stress parameters, such as catalase and peoxidase activity, content of ascorbic acid, phenols and flavonoids in bean plants. A laboratory pot experiment was carried out on samples of light silty loam containing 1.2% of humus. Pots were filled with 1 kg soil samples each, to which NaCl solution was added in doses 10, 30 and 50 mM kg-1. Each pot was seeded with 7 seeds of cv. Aura bean. The plants grown in soil without NaCl were the control. On days 14, 21 and 28 green parts of plants were collected for determinations of catalase and peroxidase activity by colorometry as well as the content and flavonoids, phenols, ascorbic acid and chloride concentration by Mohr’s method. The results show that chloride concentration in bean plants increased at higher of NaCl concentration in soil. The activity of the antyoxidative enzymes such as catalase and peroxidase in bean plants, on sampling days, was higher as the chloride concentration in plants increased. Non-enzymatic antioxidants: flavonoids, phenols and ascorbic acid content during the experiment showed different changes with relation to the chloride content, but in all the trials ascorbic acid content was significantly positively correlated whereas the content of phenols was significantly negatively correlated with the chloride content in plant tissues.
6

The role of Ca ions in changes induced by excess Cu2plus in bean plants. Growth parameters

80%
Maksymiec W., Baszynski T.
Acta Physiologiae Plantarum
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1998
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tom 20
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nr 4
The effect of Ca on Cu toxicity in runner bean plants (Phaseolus coccineus L. cv. Piěkny Jaś) grown hydroponically in nutrient solution was studied. The toxic effect of excess Cu on plants depends on their age and Ca content in the medium. Copper applied in excess to the plants at the early phase of leaf development strongly limits the uptake of Ca ions from the nutrient solution, particularly their translocation to leaves. Increased Ca content limits the inhibitory effect of Cu on leaf growth and decreases the content of chloroplast pigments to the level approximate to that of control. At this growth stage the effect of excess Cu is at least partially connected with limited Ca transport to leaves. At the intermediate leaf phase Cu-treated plants react slightly to changed Ca content. At the end of the primary leaf development increased Ca concentration in the medium intensifies senescence processes induced by excess Cu. The changes are partially connected with intensified water deficit. Increased Ca content in the nutrient solution limits Cu accumulation in the individual organs of Cu-treated plants. However, Cu accumulation in leaves is not decreased at a high level of Ca. Copper generally decreases Ca content in the youngest plants, whereas in the oldest ones only in the case of a low level of Ca in the nutrient solution.
7

The effects of excessive exposure to copper in bean plants

80%
Yurekli F., Banu Porgali Z.
Acta Biologica Cracoviensia. Series Botanica
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2006
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tom 48
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nr 2
The present study aimed to identify changes in important physiological events related to Cu, malondialdehyde (MDA), nitric oxide (NO), chlorophyll a and chlorophyll b content in the antioxidative defense system in bean seedlings (Phaseolus vulgaris L. cv. Akman) after Cu treatment. The activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were determined. Cu excess was induced in Phaseolus vulgaris (cv. Akman) plants by soaking the roots in 100 µM CuSO4 solution for 10 days. Cu content increased in roots, and nitric oxide levels increased remarkably in leaf tissue. Changes in enzyme activity and MDA were observed in root tissue. The highest accumulation of NO was observed in leaf tissue. The study included an assessment of the correlation between heavy metal accumulation in roots, leading to different manifestations of stress, and changes in chlorophyll level. Indications of oxidative stress response were detected by monitoring changes in the activity and content of some components of the antioxidative mechanism. Cu treatment increased the activity of superoxide dismutase, peroxidase and catalase in leaf tissue.
8

Alteration in growth and some relevant metabolic processes of broad bean plants during extreme temperatures exposure

70%
Hamada A. M.
Acta Physiologiae Plantarum
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2001
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tom 23
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nr 2
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