The purified aurochs (Bison bonasus, European bison) heart pyruvate dehydrogenase complex (PDC) has a set of subunits typical of mammalian PDC. PDC from aurochs heart contains firmly bound tiamine pyrophosphate in the amount providing over 50% of the maximal activity of the complex. The apparent value for activation energy of PDC is 60 kJ/mol. The Michael is constant values for aurochs heart PDC are 22.4 ± 1.0,3.3 ± 0.1 and 24.4 ± 3.6 uM for pyruvate, CoA and NAD, accordingly. Acetyl-CoA is a competitive inhibitor with respect to CoA (Ki = 14.2 ± 0.4 uM), whereas NADH gives the same inhibition with respcct to NAD (Ki = 46.9 +10.0 uM). The Km for CoA and NAD of the aurochs heart PDC are lower than that of domestic animals PDC.
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