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1

Impact of seed-transmitted viruses on quality of cereal seeds

100%
Jezewska M., Trzmiel K.
Journal of Plant Protection Research
|
2009
|
tom 49
|
nr 4
460-464
Among seed-transmitted cereal viruses the most important is Barley stripe mosaic virus (BSMV), infecting barley. Investigations on the occurrence of BSMV in Poland have been carried out in the Institute of Plant Protection in Poznań since 2000. The studies aimed at the evaluation of the BSMV distribution in plant material, the rate of its seed transmission in different barley cultivars and the assessment of the effect of barley infection on the yield. The potential risk of BSMV occurrence for barley crops was discussed. The rate of infection by two seed-transmitted viruses, Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV), in Poland was investigated in view of the risk assessment.
2
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The impacts of BSMV on vegetative growth and water status in hulless barley (Hordeum vulgare var. nudum) in VIGS study

100%
Liang J., Chen X., Zhao H., Yu S., Long H., Deng G., Pan Z., Yu M.
Acta Societatis Botanicorum Poloniae
|
2015
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tom 84
|
nr 1
Barley stripe mosaic virus (BSMV) is an established and extensively used virus-induced gene silencing (VIGS) vector for gene function analysis in monocots. However, the phenotypes generated by targeted gene silencing may be affected or masked by symptoms of BSMV infection. To better understand the potential effects of BSMV-VIGS in hulless barley (Hordeum vulgare var. nudum), the accumulation pattern of BSMV and its impacts on vegetative growth and water status were investigated. The results indicated that the vegetative growth of infected plants was significantly and continuously impacted by BSMV from 10 to 40 days post inoculation (dpi). When the accumulation of BSMV was extremely high (7 to 11 dpi), infected plants displayed twisted leaf tips with an increased water lose rate (WLR) and decreased water content (WC). Virus accumulation declined and stabilized after 25 dpi, at this stage, the WLR and WC were unaffected in the infected plants. The efficiency of VIGS was tested by the silencing of Phytoene desaturase (PDS). RT-qPCR indicated that BSMV-VIGS can be sustained with good efficiency for up to 40 dpi under an altered condition with lower temperature (22 ±1°C) and higher relative humidity (70 ±10%). It was concluded that 25 to 40 dpi was the appropriate time zone for drought-related gene analysis by BSMV-VIGS under such condition.
3

Brome mosaic virus [BMV] and barley stripe mosaic virus [BSMV] - based vectors: application in gene silencing in plants

100%
Barciszewska-Pacak M., Pacak A., Florek B., Alejska M., Lacomme C., Szweykowska-Kulinska Z., Figlerowicz M., Jarmolowski A.
Biological Letters
|
2005
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tom 42
|
nr 2 Spec.Vol.
4

A new BSMV-based vector with modified beta molecule allows simultaneous and stable silencing of two genes

80%
Kawalek A., Dmochowska-Boguta M., Nadolska-Orczyk A., Orczyk W.
Cellular and Molecular Biology Letters
|
2012
|
tom 17
|
nr 1
Virus-induced gene silencing is an important tool for functional gene analysis and the vector based on Barley stripe mosaic virus (BSMV) is widely used for the purpose in monocots. Of the tripartite BSMV genome, currently the BSMV:γMCS molecule is used to clone a fragment of a target gene. As an alternative, the BSMV:β molecule was engineered with a unique BamHI site between the open reading frame of βc (ORF βc) and poly(A). The mixture of RNA particles α, βBamHI and γMCS was fully infectious. Barley phytoene desaturase and wheat phospholipase Dα fragments were cloned to βBamHI and γMCS. Delivery of the target gene fragment in γMCS induced stronger silencing, while delivery in βBamHI yielded more stable transcript reduction. A quantitative analysis (qRT-PCR) of the transcripts showed that the silencing induced with a fragment carried in both particles was stronger and more stable than that from a fragment placed in one particle. The modification of β enables simultaneous silencing of two genes. Quantifying the β and γ particles in virus-inoculated plants revealed a 2.5-fold higher level of γ than β, while the stability of the insert was higher in β compared with γ. The possible influence of the relative quantity of β and γ particles in virus-inoculated plants on insert stability and gene silencing efficiency is discussed.
5

Z badan nad wirusami zboz prowadzonych w Instytucie Ochrony Roslin w latach 1991-2000

80%
Jezewska M.
Progress in Plant Protection
|
2001
|
tom 41
|
nr 1
273-277
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Identification of Barley stripe mosaic virus in Poland

80%
Jezewska M.
Journal of Plant Protection Research
|
2001
|
tom 41
|
nr 2
Investigations on the occurrence of Barley stripe mosaic virus (BSMV, Hordeivirus) in Poland were performer by testing seeds of 22 barley cultivars. BSMV was detected in seeds of winter barley cv. Tiffany and of spring barley cvs. Scarlett and Stratus. The virus presence was revealed by ELISA test and then confirmed by electron microscopy. Preliminary data on the rate of seed transmission of BSMV in cvs. Scarlett and Stratus are presented.
7

Wirus pasiastej mozaiki jęczmienia (Barley stripe mosaic virus, BSMV) – utajony patogen jęczmienia

71%
Jezewska M., Cajza M.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 1
Barley stripe mosaic virus (BSMV) was found in seeds of 24 varieties originated from barley cultivar collections per 40 tested. The virus more frequently occurred in seeds of winter cultivars where it was detected in seeds of 17 varietes per 19 tested while in seeds of spring cultivars it was found only in 7 cultivars per 21 tested. Similar results were obtained in the case of commercial varieties. The infection of seeds of some varieties exceeded 10%. In all cases occurrence of the mild and no symptoms was confirmed. Preliminary studies showed that BSMV caused the decrease of yield by 9–15% and the decrease of mass of 1 000 seeds ranging from 0.5 to 3%. The risk of isolate mutation of a mild BSMV to the isolate causing severe symptoms was observed in spring barley cultivar Annabell.
8

Podlegaja obowiazkowi zwalczania...

31%
Wolny S.
Agrochemia
|
1999
|
nr 07
37-41
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